A kind of soil heavy metal remediation agent and its preparation and application
A technology for heavy metals and remediation agents, applied in applications, soil conditioning materials, other chemical processes, etc., can solve the problems of low stability of the remediation effect of single functional bacteria, narrow tolerance range of heavy metal cadmium, and inability to promote microbial proliferation, etc. Significant yield increase, beneficial to passivation and improvement of soil acidification
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Embodiment 1
[0035] Embodiment 1, bacterial strain and soil heavy metal remediation agent are to Cd 2+ The dynamic adsorption effect of
[0036] Secondary fermentation of Issaccharomyces orientalis, Bacillus licheniformis and Paecilomyces lilacinus were inoculated into Cd 2+ Concentration (g / L) in the fermentation medium of 0.2, 0.4, 0.6, 0.8, 1.0, at 28 ℃, 180rpm shaking culture 24h, sampling 6000rpm centrifugation 10min, take the supernatant and use atomic spectrophotometry to measure the fermentation broth and control The content of heavy metal cadmium in the medium (without inoculating strains) was used to calculate the removal rate.
[0037] Issaccharomyces orientalis, Bacillus licheniformis, Paecilomyces lilacinus contain Cd 2+ In the fermentation broth with a concentration of 0.2-1g / L, after 24 hours of fermentation, the removal rate of cadmium is more than or equal to 50%. In the 0.4g / L cadmium solution, the removal rate of I. orientalis to cadmium can reach up to 74.25%, the re...
Embodiment 2
[0043] Embodiment 2, the restoration effect of soil heavy metal remediation agent to different degrees of cadmium polluted soil
[0044] Adopt 0.3mg / kg (treatment 1), 3mg / kg (treatment 2), 15mg / kg (treatment 3) self-made cadmium contaminated soil to carry out soil culture test, each treatment experiment is set up two groups, one group is control group (not using Soil heavy metal remediation agent), another group is the experimental group (application of soil heavy metal remediation agent), and each group of experiments is repeated three times. The prepared soil heavy metal remediation agent was added to the cadmium-contaminated soil, mixed well, the soils of different treatments were quantitatively weighed and placed in pots for cultivation, watered regularly, and the soil humidity was kept to 60% of the field water capacity. Samples were taken at 0d, 15d, and 40d. After the soil samples were air-dried, the available cadmium content in the soil was detected.
[0045] Table 2 ...
Embodiment 3
[0050] Embodiment 3, pot test application effect of soil heavy metal remediation agent
[0051] 1 test treatment
[0052] The test plant was lettuce, and the seedlings with the same growth were selected for the pot experiment. The pot soil was self-made cadmium-contaminated soil. The experiment consisted of 8 treatments, and the difference in the application amount of the bacterial solution between the treatments was made up with sterilized medium, and repeated 5 times. times, 5-6 strains per pot, and the experimental treatment design is shown in Table 4.
[0053] Table 4 Treatment design of lettuce pot experiment
[0054]
[0055] Note: Paecilomyces lilacinus, Issaccharomyces orientalis, and Bacillus licheniformis are referred to as Lavender, Lichen, and Orient respectively.
[0056] 2 Results and Analysis
[0057] Each treatment was placed in a greenhouse with the functions of shelter from rain, ventilation, and temperature regulation. Daily spray watering was carried ...
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