Preparation method of immobilized microbial formaldehyde degradation preparation

Abstract

The invention discloses a preparation method of an immobilized microbial formaldehyde degradation preparation, belonging to the technical field of formaldehyde degradation. The preparation method comprises the following steps: mixing kitchen waste with active sludge, then mixing with a prepared culture medium, and culturing; after the culture, sequentially adding a formaldehyde solution, a sodium sulfide solution, a sodium sulfite solution and a sodium hydrogen sulfite solution, and performing aeration and acclimation culture; after the culture, picking out the vigorously growing bacteria for purification and enrichment culture to obtain bacterial liquid capable of degrading formaldehyde; and sterilizing wormcast and solidifying the bacterial liquid to obtain the immobilized microbial formaldehyde degradation preparation. In the invention, the bacteria after culture and acclimation are immobilized in a carrier, the obtained preparation can thoroughly decompose formaldehyde, the formaldehyde removal rate is high, and secondary environmental pollution is avoided during the purification and degradation.

Description

technical field

[0001] The invention discloses a preparation method of an immobilized microorganism degrading formaldehyde preparation, which belongs to the technical field of formaldehyde degradation. Background technique

[0002] In recent years, indoor formaldehyde pollution has attracted more and more attention. Formaldehyde is a colorless, irritating, toxic gas that can induce various diseases and pose serious hazards to human health. Therefore, it is imminent to find an economical, environmentally friendly and simple method to deal with indoor formaldehyde pollution. At present, the methods for degrading indoor formaldehyde include enhanced ventilation, physical adsorption, plant decomposition, and chemical methods, all of which have their own advantages and limitations. The biological method is a relatively convenient and environmentally friendly formaldehyde removal method, but this method is greatly affected by the activity of microorganisms and the living environ...

Examples

preparation example Construction

[0019] The preparation method of the slant medium: Weigh 5-7g peptone, 1-3g yeast extract powder, 0.1-0.3g ferric phosphate, 20-30g agar, 5-7g sodium chloride, 1000mL sterile water, stir Heat until dissolved, adjust the pH to 7.2 with a concentration of 0.5mol / L sodium hydroxide solution, divide the culture solution into test tubes, seal them with cotton plugs, and sterilize the test tubes at 120-125°C for 15-20 minutes. Before the sterilized culture medium is solidified, the test tube is tilted into a 45° slope with the horizontal plane, and the culture medium solution is naturally solidified to room temperature to obtain the slope culture medium;

[0020] The preparation method of the slant medium: Weigh 10-15g of peptone, 10-15g of sodium chloride, 5-7g of yeast extract powder and 100-200mL of sterile water, adjust the pH to 7.0 after stirring and mixing, and add 1L of In the bottle, dilute to the mark with sterile water and sterilize at 120-125°C for 5-10 minutes.

example 1

[0022]Weigh 500g of kitchen waste and put it into a plastic bucket, add 3L of tap water to the plastic bucket, seal the plastic bucket after stirring and mixing evenly, place it in a dark environment for 5 days, filter after storage, and remove the filtrate to obtain Filter residue: Weigh 200g of activated sludge from the aeration tank of the polyoxymethylene plant and add it to the fermentation kettle, add 500mL of deionized water and 30g of brown sugar to the fermentation kettle, stir and mix and leave it to ferment for 3 days at a temperature of 25°C. After fermentation, The fermented product was mixed with the above filter residue and stirred evenly to obtain the mixture; respectively weigh 40g of peptone, 35g of beef extract, 20g of yeast extract, 25g of glucose, 5g of Tween 80, 1g of ammonium citrate, 3g of sodium acetate, and 0.1g of Add magnesium sulfate, 0.01g manganese sulfate, and 1g dipotassium hydrogen phosphate into a 1L volumetric flask, add distilled water to th...

example 2

[0027] Weigh 600g of kitchen waste and put it into a plastic bucket, and add 4L of tap water to the plastic bucket, stir and mix evenly, seal the plastic bucket, place it in a dark environment for 6 days, filter after the storage, and remove the filtrate to obtain filter residue; take 250g of polyoxymethylene plant aeration tank activated sludge and add it to the fermentation kettle, add 600mL deionized water and 40g brown sugar to the fermentation kettle, stir and mix and leave it to ferment for 4 days at a temperature of 26°C. After fermentation, The fermented product was mixed with the above-mentioned filter residue and stirred evenly to obtain the mixture; 45g of peptone, 40g of beef extract, 23g of yeast extract, 30g of glucose, 7g of Tween 80, 2g of ammonium citrate, 3.5g of sodium acetate, 0.2 Add 1 g of magnesium sulfate, 0.02 g of manganese sulfate, and 1.5 g of dipotassium hydrogen phosphate into a 1L volumetric flask, add distilled water to the volume, adjust the pH ...

technical field

[0001] The invention discloses a preparation method of an immobilized microorganism degrading formaldehyde preparation, which belongs to the technical field of formaldehyde degradation. Background technique

[0002] In recent years, indoor formaldehyde pollution has attracted more and more attention. Formaldehyde is a colorless, irritating, toxic gas that can induce various diseases and pose serious hazards to human health. Therefore, it is imminent to find an economical, environmentally friendly and simple method to deal with indoor formaldehyde pollution. At present, the methods for degrading indoor formaldehyde include enhanced ventilation, physical adsorption, plant decomposition, and chemical methods, all of which have their own advantages and limitations. The biological method is a relatively convenient and environmentally friendly formaldehyde removal method, but this method is greatly affected by the activity of microorganisms and the living environ...

Examples

preparation example Construction

[0019] The preparation method of the slant medium: Weigh 5-7g peptone, 1-3g yeast extract powder, 0.1-0.3g ferric phosphate, 20-30g agar, 5-7g sodium chloride, 1000mL sterile water, stir Heat until dissolved, adjust the pH to 7.2 with a concentration of 0.5mol / L sodium hydroxide solution, divide the culture solution into test tubes, seal them with cotton plugs, and sterilize the test tubes at 120-125°C for 15-20 minutes. Before the sterilized culture medium is solidified, the test tube is tilted into a 45° slope with the horizontal plane, and the culture medium solution is naturally solidified to room temperature to obtain the slope culture medium;

[0020] The preparation method of the slant medium: Weigh 10-15g of peptone, 10-15g of sodium chloride, 5-7g of yeast extract powder and 100-200mL of sterile water, adjust the pH to 7.0 after stirring and mixing, and add 1L of In the bottle, dilute to the mark with sterile water and sterilize at 120-125°C for 5-10 minutes.

example 1

[0022]Weigh 500g of kitchen waste and put it into a plastic bucket, add 3L of tap water to the plastic bucket, seal the plastic bucket after stirring and mixing evenly, place it in a dark environment for 5 days, filter after storage, and remove the filtrate to obtain Filter residue: Weigh 200g of activated sludge from the aeration tank of the polyoxymethylene plant and add it to the fermentation kettle, add 500mL of deionized water and 30g of brown sugar to the fermentation kettle, stir and mix and leave it to ferment for 3 days at a temperature of 25°C. After fermentation, The fermented product was mixed with the above filter residue and stirred evenly to obtain the mixture; respectively weigh 40g of peptone, 35g of beef extract, 20g of yeast extract, 25g of glucose, 5g of Tween 80, 1g of ammonium citrate, 3g of sodium acetate, and 0.1g of Add magnesium sulfate, 0.01g manganese sulfate, and 1g dipotassium hydrogen phosphate into a 1L volumetric flask, add distilled water to th...

example 2

[0027] Weigh 600g of kitchen waste and put it into a plastic bucket, and add 4L of tap water to the plastic bucket, stir and mix evenly, seal the plastic bucket, place it in a dark environment for 6 days, filter after the storage, and remove the filtrate to obtain filter residue; take 250g of polyoxymethylene plant aeration tank activated sludge and add it to the fermentation kettle, add 600mL deionized water and 40g brown sugar to the fermentation kettle, stir and mix and leave it to ferment for 4 days at a temperature of 26°C. After fermentation, The fermented product was mixed with the above-mentioned filter residue and stirred evenly to obtain the mixture; 45g of peptone, 40g of beef extract, 23g of yeast extract, 30g of glucose, 7g of Tween 80, 2g of ammonium citrate, 3.5g of sodium acetate, 0.2 Add 1 g of magnesium sulfate, 0.02 g of manganese sulfate, and 1.5 g of dipotassium hydrogen phosphate into a 1L volumetric flask, add distilled water to the volume, adjust the pH ...