Yeast products with antioxidation and selenium-supplementing functions and preparation method thereof

A technology of anti-oxidation and selenium supplementation, applied in microorganism-based methods, biochemical equipment and methods, and microbial dissolution, etc., can solve the problems of low absorption rate, only attention to selenium absorption and utilization rate, and large molecular weight of selenoproteins. To achieve the effect of promoting absorption and helping digestion and absorption

Active Publication Date: 2018-01-16

WUHAN POLYTECHNIC UNIVERSITY

8 Cites 5 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0003] However, the current use of selenium-enriched yeast in the industry has not been optimized. The specific problems are as follows: 1. The selenium absorption rate of active yeast used in the market is not high in piglets. The main reason is that yeast cell wall cracking requires strong enzymes The role of yeast autolysis in the intestinal tract requires a certain process, and the release of selenoproteins in yeast in the digestive tract is often not thorough enough

2. The selenoprotein obtained by yeast autolysis has a large molecular weight, such as selenoprotein P (Selenoprotein P) contains 10 molecules ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Abstract

The invention discloses a yeast product with antioxidation and selenium-supplementing functions and a preparation method thereof. The preparation method comprises the following steps: (1) adding sodium selenite to saccharomyces serevisiae in a logarithmic growth phase and/or the growth stationary phase, obtaining bacterial liquid after the fermentation, and centrifuging and drying obtained thalli;(2) carrying out inactivation on the obtained thalli to ensure that endoenzyme of the saccharomyces serevisiae keeps active while the saccharomyces serevisiae is dead; (3) adding neutral protease, papain and NaCl into inactivated thalli, carrying out enzymolysis autolysis under an anaerobic condition; (4) collecting the autolysis solution obtained in step (3) for centrifugation, and carrying outfreeze-drying on the obtained precipitate, thereby obtaining a yeast product. The yeast product produced by the preparation method provided by the invention has high selenium content, simultaneously possesses the antioxidation property, and helps animals to digest and absorb organic selenium, and is a yeast product which has the antioxidation and selenium supplementing functions.

Application Domain

Microorganism lysisAnimal feeding stuff +2

Technology Topic

ChemistryEndoenzyme +11

Image

Examples

Experimental program(10)
Comparison scheme(6)
Effect test(1)

Example Embodiment

[0025] The preparation method of yeast product in the embodiment of the present invention 1-9 is:

[0026] Add 20 μg/ml sodium selenite to the basal medium in six times at 4h, 7h, 10h in the logarithmic phase of growth of brewer’s yeast and 12h, 14h, and 16h in the stable growth phase, at a temperature of 30°C, shake the flask for 24h, and collect Bacteria liquid, centrifuged and dried to obtain bacteria, the selenium content of the obtained bacteria is 4000mg/kg; control humidity 80%, inactivate at 55°C for 2 hours, to ensure the death of yeast and keep the activity of intracellular enzymes; enter the enzymolysis tank, enzymolysis Before the tank is used, pre-exclude oxygen, fill with a certain volume of nitrogen, take the inactivated bacteria as the benchmark, add 0.5w% neutral protease, 0.3w% papaya enzyme, 0.3w% NaCl, adjust to pH = 6.0, temperature 55 Autolyze at ℃ for 20 hours; drop to 4℃ and immediately collect from the solution for centrifugation at a centrifugation speed of 3000r/min for 20 minutes, dry the precipitate in a vacuum at low temperature to obtain the yeast product vacuum bagged for use.

Example Embodiment

[0027] Example 1:

[0028] The yeast product was added to the base material at a dosage of 0.2 mg/kg, and the feed was delivered on 20 days.

[0029] The ingredients of the above-mentioned base material are: 45% of corn, 15% of soybean meal, 10% of rapeseed meal, 10% of rice bran meal, 15% of wheat bran, 4% of premixed feed, and 1% of soybean oil.

[0030] The formula of the above premixed feed is: vitamin premix 25‰, trace element premix 125‰, lysine 50‰, choline 30‰, pig compound enzyme 35‰, calcium 4.5‰, phosphorus 1.5‰, salt 3 ‰, flavoring agent 36‰, stone powder 200‰, calcium hydrogen phosphate 240‰, carrier 250‰.

[0031] The active ingredients of the above vitamin premix are: vitamin A6750IU/kg, vitamin D1500IU/kg, vitamin E30mg/kg, vitamin K1.5mg/kg, vitamin B 1 1.5mg/kg, vitamin B 2 3.8mg/kg, vitamin B 6 2.3mg/kg, vitamin B 12 17μg/kg, niacin 15mg/kg, calcium pantothenate 7.5mg/kg, folic acid 0.8mg/kg, biotin 0.12mg/kg.

[0032] The active ingredients of the above trace element premix are copper 15mg/kg, iron 90mg/kg; manganese 30mg/kg, zinc 90mg/kg, cobalt 0.5mg/kg, iodine 0.2mg/kg, selenium 0.2mg/kg, carrier 862.41‰ .

Example Embodiment

[0033] Example 2:

[0034] The difference from Example 1 is that the animals were fed within 30 days.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

Classification and recommendation of technical efficacy words

Promote absorption
Aids in digestion and absorption

Yeast products with antioxidation and selenium-supplementing functions and preparation method thereof

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Spontaneous-heating thermal insulation eye mask

Mini-sized agricultural mist culture device

Seaweed polysaccharide beverage

Treating method and product of organic osmanthus green tea

Microwave oven with touch screen

Classification and recommendation of technical efficacy words

Three-dimensional substrate comprising a tissue layer

Compositions for improving gastrointestinal nutrient and drug absorption

Semiconductor device and manufacturing method thereof

Method and apparatus for improving fluidic flow and sample capture

Absorbent article having fused layers

Health care medicinal liquor and preparation method thereof

Making method of yoghurt sandwich biscuits

Chinese yam nutritional essence and preparation method thereof

Green flavored feed for laying hens

Chicken feed additive capable of prolonging egg-laying peak period