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Method for detecting vitamin C in food with fluorescent/NMR dual-functional probe

A dual technology of vitamins and NMR, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of expensive and limited use

Active Publication Date: 2021-07-06
DALIAN POLYTECHNIC UNIVERSITY
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Problems solved by technology

[0002] The analysis and detection of nutrients in food is the basic technology in food science research. Nutrients in food include six major nutrients: carbohydrates, fats, proteins, vitamins, inorganic salts and water. Vitamin C, also known as ascorbic acid ( AA), rich in fruits and vegetables, vitamin C can be used as an enzyme cofactor, a reducing agent, a component of neurotransmitter-related enzymes and a necessary nutritional factor, which is very important for maintaining normal human physiological functions. At the same time, vitamin C It is also widely used in many fields, such as as an antioxidant in food, animal feed, beverage, cosmetics and pharmaceutical formulations. The lack of vitamin C in the human body can lead to various diseases, including the common cold, scurvy, mental illness, infertility However, vitamin C is an exogenous chemical substance that cannot be synthesized in the human body and can only be obtained from food to maintain the required content of the human body. Therefore, the detection of vitamin C is important for the diagnosis of diseases and daily life. The evaluation of the nutritional value of food in life is of great significance. So far, many methods for the detection of vitamin C have been developed at home and abroad, including titration, spectrophotometry, high performance liquid chromatography, chemiluminescence, and DPPH. and phenylhydrazine colorimetric method, etc., however, their use in rapid detection is limited due to the need for expensive equipment and complicated pretreatments. Therefore, it is highly desirable to develop a simple, sensitive, high-selectivity and A low-cost dual-function detection method. Nanomaterials have attracted widespread attention due to their unique properties. Different nanomaterials have been widely studied and applied to the analysis and detection of different substances. Among them, carbon nanomaterials have good biocompatibility. , tunable excitation and emission spectra, and high stability make it a very promising sensor

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  • Method for detecting vitamin C in food with fluorescent/NMR dual-functional probe
  • Method for detecting vitamin C in food with fluorescent/NMR dual-functional probe
  • Method for detecting vitamin C in food with fluorescent/NMR dual-functional probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of carbon nanofluorescent probes: 0.5 g citric acid and 0.5 g glycine were dissolved in 10 mL water, and then the solution was transferred to a poly(tetrafluoroethylene)-lined autoclave at 180 °C in After heating in an oven for 24 hours and naturally cooling to room temperature, a dark brown suspension containing fluorescent carbon nanomaterials was obtained, which was centrifuged at 5000 rpm for 5 minutes to remove large particles, and purified using a D101 macroporous adsorption resin column with water as the eluent The crude product, the fluorescent carbon nanomaterial, was concentrated by a vacuum rotary evaporator, and then freeze-dried to obtain a purified fluorescent carbon nanomaterial as a white powder, which was examined by a transmission electron microscope (TEM) as figure 1 , it can be observed that the dispersion of fluorescent carbon nanoparticles is good, and the particle size distribution is between 1-5 nm, such as figure 2 , with...

Embodiment 2

[0034] Embodiment two, MnO 2 Preparation of aqueous solution of nanosheets: fully dissolve 0.5 g potassium permanganate in 500 mL deionized water, add 1.5 g hexadecyl trimethyl potassium bromide to it, stir continuously, and add morphine B at pH=6 continuously dropwise. Mix 50 mL of sulfonic acid and sodium hydroxide, continue to stir for 12 h, centrifuge the obtained brown-black mixture (10000 rpm for 5 min) to collect the precipitate, rinse the precipitate with deionized water and redissolve, repeat 3-5 times to obtain MnO 2 Aqueous solutions of nanosheets, such as Figure 5 , MnO can be observed by TEM 2 The nanosheets present a sheet-like distribution, the size of the nanosheets is about 1.05 μm, and the Zeta potential is -15.36mV, such as Image 6 ; The concentration of fixed carbon nanomaterials is added to it with different concentrations of MnO 2 nanosheet solution, such as Figure 7 , it can be seen that with MnO 2 As the concentration of nanosheets increases, th...

Embodiment 3

[0035] Embodiment three, the establishment of the standard curve between vitamin C concentration and fluorescence intensity: take the fluorescent carbon nanomaterial in embodiment one, prepare the carbon nanomaterial aqueous solution that concentration is 2 mg / mL, take the MnO in embodiment two 2 Nanosheets were diluted in aqueous solution to a concentration of 240 μM MnO 2 Nanosheet aqueous solution, prepare BR buffer solution with pH=7, take another 13 EP tubes and add 0.5 mL of 2 mg / mL carbon nanomaterial aqueous solution, 0.5 mL of MnO with a concentration of 240 μM 2 Nanosheet aqueous solution, 0.8mL of pH=7 BR buffer solution, and then add 0.2 mL of 0, 5, 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100 μM vitamin C aqueous solution, after mixing well, let it stand at room temperature for 2 minutes, and use a fluorescence photometer with a detection wavelength range of 280-700 nm to detect the fluorescence intensity F in the standard solution 1 , the detection condition is t...

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Abstract

The invention discloses a method for detecting vitamin C in food with a fluorescent / nuclear magnetic dual-function probe. The specific steps are: A. using citric acid and glycine as raw materials, reacting in a hydrothermal synthesis reactor; B. cooling after the reaction is completed Collect the liquid phase at room temperature, and purify it through macroporous adsorption resin; C, concentrate by rotary evaporation, and freeze-dry the concentrated solution to obtain a white powdery substance, which is a carbon nanomaterial, which is stored in a dry environment for future use; D, with high Potassium manganate, hexadecyl trimethyl potassium bromide, morphine ethanesulfonic acid, and sodium hydroxide were used as raw materials to prepare MnO at room temperature 2 nanosheets, the mixture was centrifuged after stirring, and the precipitate was washed with deionized water to obtain MnO 2 Aqueous solution, after measuring the concentration by ultraviolet light, dilute to an appropriate concentration for later use; 2 Nanosheet aqueous solution, buffer composition. The invention has low cost, simple and safe probe preparation process, and can realize the detection of the vitamin C content through fluorescence and nuclear magnetic signals.

Description

technical field [0001] The invention relates to a method for detecting vitamin C in food with a fluorescent / nuclear magnetic dual function probe. Background technique [0002] The analysis and detection of nutrients in food is the basic technology in food science research. Nutrients in food include six major nutrients: carbohydrates, fats, proteins, vitamins, inorganic salts and water. Vitamin C, also known as ascorbic acid ( AA), rich in fruits and vegetables, vitamin C can be used as an enzyme cofactor, a reducing agent, a component of neurotransmitter-related enzymes and a necessary nutritional factor, which is very important for maintaining normal human physiological functions. At the same time, vitamin C It is also widely used in many fields, such as as an antioxidant in food, animal feed, beverage, cosmetics and pharmaceutical formulations. The lack of vitamin C in the human body can lead to various diseases, including the common cold, scurvy, mental illness, infertili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/65B82Y40/00B82Y30/00
CPCB82Y30/00B82Y40/00C09K11/65G01N21/6408
Inventor 王海涛那晓康刘赫非江籽橙谢明征李昱韩非谭明乾程沙沙
Owner DALIAN POLYTECHNIC UNIVERSITY
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