Caproic acid bacterium proliferation culture medium and caproic acid fermentation and caproic acid bacterium screening method
A technology of proliferation medium and fermentation method, which is applied in the fields of microbial fermentation and functional bacteria screening and identification, can solve the problems of quantitative detection error, negative influence of color development effect, easy to contaminate miscellaneous bacteria, etc., so as to reduce the toxic effect and improve bacteria. The effect of density
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[0078] Example 1:
[0079] The proliferation, fermentation and detection methods of the caproic acid screening system provided by the embodiments of the present invention specifically include:
[0080] Step one, prepare LRCM medium (containing ascorbic acid). The ingredients of LRCM liquid medium are: peptone 10.0g / L, beef powder 10.0g / L, yeast powder 3.0g / L, glucose 5.0g / L, soluble starch 1.0g / L, sodium chloride 5.0g / L, sodium acetate 3.0g / L, L-cysteine hydrochloride 0.5g / L, ascorbic acid 0.3g / L, agar 0.5g / L, distilled water 1000mL, pH value 6.8±0.1. Dispense the liquid medium into 100 mL anaerobic bottles.
[0081] Step two, autoclave (121℃, 20min), when cooled to 50~55℃, add pre-sterilized liquid seal oil to isolate air (see figure 2 a). Liquid seal oil components: liquid paraffin and petrolatum (ratio 3:1).
[0082] Step three, inoculate 1 mL of YL-19 culture solution of the tested strain (see image 3 a). Insert a sterilized needle between the cap and the sealing film to p...
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[0088] Example 2:
[0089] The proliferation, fermentation and detection methods of the caproic acid screening system provided by the embodiments of the present invention include:
[0090] Step one, prepare 100mL LRCM medium. The ingredients of LRCM liquid medium are: peptone 10.0g / L, beef powder 10.0g / L, yeast powder 3.0g / L, glucose 1.0g / L, soluble starch 1.0g / L, sodium chloride 5.0g / L, sodium acetate 5.0g / L, L-cysteine hydrochloride 0.5g / L, ascorbic acid 1.0g / L, agar 1.0g / L, distilled water 1000mL, pH 6.8±0.1. Dispense the liquid medium into 100 mL anaerobic bottles.
[0091] Step two, autoclave (121℃, 20min), when cooled to 60~70℃, add vitamin B10.5g / L and hemin 0.01g / L. Add pre-sterilized liquid seal oil to isolate air. The components of liquid sealing oil are: liquid paraffin and petrolatum (5:1).
[0092] Step three, inoculate 2.0 mL of the culture solution of the tested strain XL-48a. Insert a sterilized needle between the cap and the sealing film to prevent explosion ca...
Example Embodiment
[0098] Example 3:
[0099] The proliferation, fermentation and detection methods of the caproic acid screening system provided by the embodiments of the present invention include:
[0100] Step one, prepare 11.5L of LRCM liquid medium. LRCM medium components are: peptone 10.0g / L, beef powder 10.0g / L, yeast powder 3.0g / L, glucose 5.0g / L, soluble starch 1.0g / L, sodium chloride 5.0g / L, sodium acetate 6.0 g / L, L-cysteine hydrochloride 0.5g / L, ascorbic acid 0.5g / L, carrageenan 1.0g / L, distilled water 1000mL, pH value 6.8±0.1. Divide the liquid medium into 115 anaerobic bottles with a volume of 100 mL.
[0101] Step two, autoclave (121°C, 20min), when cooled to 50-70°C, add pre-sterilized liquid seal oil to isolate the air. Liquid seal oil components are: liquid paraffin and petrolatum (6:1).
[0102] Step three, use the inoculation loop to pick XL-34a caproic acid bacteria (see image 3 b, 3c) and other single colonies, a total of 115 fermentation systems were prepared (see figure 2 ...
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