Separation and application of microorganisms for treating wastewater
A technology of microbial strains and wastewater, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems that restrict the production and application of folic acid, secondary pollution, environmental pollution, etc., and achieve sustainable development and good removal The effect of action
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Embodiment 1
[0044] Example 1: Screening of strains
[0045] Acidic PDA solid medium: Wash the potato skin, weigh 200g of the potato and cut it into small pieces, add water to boil (boil for 20-30 minutes, it can be punctured by a glass rod); filter with 8 layers of gauze, heat, add 20g agar; continue to heat, stir and mix, after the agar is dissolved, add glucose, stir evenly, add water to 1L after cooling slightly, sterilize at 121°C for 20min, and store after cooling for later use.
[0046] Acidic LB liquid culture medium: tryptone 10g / L, sodium chloride 10g / L, yeast extract 5g / L, add distilled water and add hydrochloric acid to adjust the pH to 1.5.
[0047] (1) The recovered folic acid wastewater is subjected to pretreatment of cooling and pH adjustment, the temperature of the wastewater is lowered to room temperature, and the pH is adjusted to 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5 in turn.
[0048] (2) Put 150 mL of folic acid wastewater with pH adjusted to 6.5 into a 500 mL conica...
Embodiment 2
[0055] Example 2: Identification of strains
[0056] Using optical microscope to observe the morphological characteristics of the strain, the results are as follows: figure 1 As shown, the thalline of the strain is rod-shaped, and the colony of the strain on the acidic PDA medium of pH 1.5 is milky white, round, moist, with complete and clear edges, convex, and the colony is opaque.
[0057] The genus was identified by Shanghai Meiji Biomedical Technology Co., Ltd. using 3730XL bidirectional sequencing method. After amplification using ITS universal primers, the primers and amplification system are as follows: the amplified bands are detected by electrophoresis. Electropherogram such as figure 2 As shown, lane 2 is the electropherogram of the strain.
[0058] (1) Primers used:
[0059] ITS1: TCCGTAGGTGAACCTGCGG,
[0060] ITS4:TCCTCCGCTTATTGATATGC.
[0061] (2) PCR amplification reaction system:
[0062]
[0063]
[0064] (3) PCR reaction conditions:
[0065] ...
Embodiment 3
[0066] Example 3: Heat tolerance of strains
[0067] (1) Inoculate the Paecilomyces spp. YS-2 obtained by screening into 10 mL of acidic LB medium (pH 1.5) at a volume ratio of 2% at 37° C. and 220 rpm for activation. YS-2 was inoculated into fresh acidic PDA medium and allowed to OD in the medium 600 is 0.1;
[0068] (2) Culture at different temperatures of 30°C, 35°C, 40°C, 45°C, 50°C, and 55°C for 24 hours;
[0069] (3) Calculate the number of viable bacteria.
[0070] As shown in Table 1, this strain has a relatively high number of viable cells at 35°C, so its optimum temperature is 35°C.
[0071] Table 1 The viable count of Paecilomyces pyogenes YS-2 at different temperatures (log CFU / mL)
[0072]
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