High-flux porous filter plate for nucleic acid synthesis and purification
A filter plate, high-throughput technology, applied in chemical/physical processes, chemical/physical/physical-chemical processes, chemical instruments and methods, etc., can solve the problems of inconsistent reaction efficiency, affecting product quality, affecting reaction effects, etc. High-quality reaction efficiency, saving synthesis reagents, and reducing the effect of dead volume
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Embodiment 1
[0045] CPG mixed polyethylene is used as the solid phase carrier filter material, which is mainly used in the chemical synthesis of DNA and RNA.
[0046] The full name of CPG is Controlled Pore Glass, that is, nanoporous glass with controllable pore size. The nanoporous glass medium is prepared by using the principle of glass phase separation, and a certain proportion of Na 2 O-B 2 o 3 -SiO 2 After the system glass is heat-treated and phase-separated at an appropriate temperature, it is soaked in inorganic acid or aqueous solution to obtain porous glass with different pore sizes and distributions. Porous glass is treated with aminomethyl groups to obtain amino groups, and then connected to the middle connecting arm through amino groups to form a solid phase carrier that can be used for DNA and RNA synthesis.
[0047]
[0048] When the porous filter plate is a 1536-well plate, the height of the reagent chamber 1 is 2-60 mm, the side length or diameter is 1-3 mm, the heigh...
Embodiment 2
[0056] C18 packing is a reversed-phase C18 extraction column based on high-purity spherical silica gel. Compared with ordinary uncapped C18 materials, spherical materials have higher uniformity and more stable flow rate; more silanol functional groups on the surface provide additional polar interactions. Also enhances the retention of basic compounds compared to capped sorbents. It is a general purpose stationary phase for the extraction of polar and nonpolar compounds.
[0057] Octadecyl bonded phase is the most commonly used bonded phase. Octadecyl chlorosilane and silanol groups on the surface of silica gel undergo multi-step reactions to produce ODS bonded phase. The bonding reaction structure is as follows:
[0058]
[0059] (R) 2 For two methyls or one methyl one chlorine or two chlorines.
[0060] Production and loading of 100 96-well plates for DNA purification, the solid phase carrier filter element has a diameter of 3mm, a thickness of 5mm, and a solid filter e...
Embodiment 3
[0067] C8 is an octyl bonded phase with completely blocked ends. It is a reverse silica gel matrix filler with moderate hydrophobicity. It retains non-polar compounds through hydrophobic interactions. Compared with C18, C8 has a shorter carbon chain and a stronger non-polar hydrophobic interaction. Weak, if the use of C18 makes it difficult to elute non-polar targets, C8 can be used for bond elution.
[0068]
[0069] (R) 2 For two methyls or one methyl one chlorine or two chlorines.
[0070] Production and loading of 100 384-well plates for DNA desalting, the solid phase carrier filter element has a diameter of 3mm, a thickness of 3.5mm, and a solid filter element with a pore size of 10um. The concentration of C8 is 5mg per capsule, which can desalt and extract nucleic acid fragments with a concentration of 8nmol-10nmol. The thickness of the membrane is 3.5mm, the length is 150mm, and the width is 150mm. A single membrane weighs 25.6g and has a volume of 33.7ml.
[0071...
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