High-flux porous filter plate for nucleic acid synthesis and purification

Abstract

The invention discloses a high-flux porous plate applied to nucleic acid synthesis and purification. The high-flux porous plate is composed of a reagent bin, a reaction bin and a solid-phase carrier filter element, the reagent bin is used for storing a to-be-reacted solution solvent, the reaction bin is used for fixing the position of the carrier filter element, and the to-be-reacted solution solvent chemically reacts with the solid-phase carrier filter element through the reaction bin and then flows out through a waste liquid opening of the reaction bin. The porous plate provided by the invention is wide in application field range, diversified in function, high in throughput sample treatment and high in production efficiency, can be widely applied to DNA synthesis and purification, RNA synthesis and purification, nucleic acid extraction and separation and life science sample treatment, and has a wide market application prospect.

Description

Technical field: [0001] The invention belongs to the field of biomedical experimental consumables, and in particular relates to a high-flux porous filter plate for nucleic acid synthesis and purification. Background technique: [0002] With the development of molecular biology, nucleic acid has been widely used as a basic raw material in medicine and food safety. Nucleic acid is a new generation of biological drugs, with multiple functions such as genetics, catalysis, energy storage, energy supply and immunity enhancement. . The use of nucleic acid can be used to design new drugs, which has broad prospects in the manufacture of anti-cancer, anti-virus, treatment of myocardial infarction and interferon-inducing agents. [0003] Nucleic acid is a class of important macromolecular compounds containing phosphate groups in organisms. Because it was originally isolated from the nucleus and is acidic, it is called nucleic acid. All living things contain nucleic acid, even viruse...

AI Technical Summary

Problems solved by technology

[0007] In the existing nucleic acid synthesis technology, the main problem is that the synthesis flux is small and cannot realize fully automated batch nucleic acid sample operation. The commercially available synthesis wells are currently mainly composed of single columns, and the operation is relatively cumbersome. A large amount of raw materials are wasted for one synthesis. This produces a large amount of organic solvent waste liquid, which is also a wasteful and cumbersome process for subsequent treatment of waste liquid

[0008] In the existing nucleic acid synthesis technology, there are mainly defects that commercially available synthetic filter plates and commercially available sample collection plates cannot be used together, and it is difficult to process samples in batches during sample processing

[0009] In the existing nucleic acid synthesis technology, there is mainly the phenomenon of sintering shrinkage in the production of the synthetic carrier filter element by particle sintering. After mixing the PE powder and filling it into a metal mold and sintering it in a high-temperature oven, a certain ratio will occur. When the carrier filter element shrinks, when it is placed in the synthesis hole, the reaction reagent reacts with the carrier filter element quickly or slowly due to the uneven size. The inconsistent reaction efficiency leads to differences in the reaction results, which affects the product quality of the final nucleic acid product.

[0010] In the existing nucleic acid synthesis technology, there is mainly the phenomenon that the dead volume is easily generated when the reagent is sprayed into the hole through the valve during the reaction process, and the residual reagent affects the reaction effect of the next step and finally affects the quality of the nucleic acid.

[0011] In the existing nucleic acid synthesis technology, there are mainly differences in the synthesis efficiency due to the inhomogeneity of the pores of the carrier filter element. This is because it is difficult to mix the powder evenly by rotating the solid powder up, down, left, and right in the process of mixing. The process of mixing solid powder is relatively long, which not only wastes time but also cannot be mixed

[0012] In the existing nucleic acid synthesis technology, the carrier mainly squeezes the filter element to a fixed position during the assembly process. Since the filter element has certain pores and is elastic, physical extrusion will change the pores of the filter element and affect the permeability of the reagent. The time of the filter element will ultimately affect the product quality

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