Polypeptide sequence bound with classical swine fever virus Erns protein and application

A peptide sequence and swine fever virus technology, applied in peptides, material inspection products, instruments, etc., can solve the problems of reaction solvent limitations, peptide non-targetability, small peptide library capacity, etc., and achieve easy synthesis, modification, and screening The effect of low strength and short development cycle
CN112625091AActive Publication Date: 2021-04-09龙湖现代免疫实验室
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
龙湖现代免疫实验室
Publication Date
2021-04-09

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Abstract

The invention mainly relates to a classical swine fever virus Erns protein affinity polypeptide and application. The polypeptide is designed by a computer-simulated virtual molecular docking technology, and the sequence is WRHYIH. According to the invention, a polypeptide sequence P1 is obtained according to a virtual molecular screening technology by taking a swine fever Erns protein crystal structure obtained by homologous modeling as a template. After the polypeptide is artificially synthesized, an enzyme-linked immunosorbent assay test and a plasma resonance test are employed to identify the affinity of the interaction of the sequence and the swine fever Erns protein, and the result shows that the sequence P1 and the swine fever Erns protein have relatively high specificity and affinity; and the invention provides a reliable theoretical basis for affinity chromatography taking affinity peptide as an affinity ligand, and swine fever Erns protein can be rapidly detected by labeling the polypeptide.
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Description

technical field

[0001] The invention mainly relates to the design and application of a polypeptide capable of specifically binding to the Erns protein of the swine fever virus, and belongs to the design of an affinity polypeptide targeting the Erns protein of the swine fever virus and its application in the detection of the swine fever virus. Background technique

[0002] Classical swine fever (CSF) is an acute, febrile and highly contagious disease caused by classical swine fever virus (CSFV). It is mainly characterized by high fever, internal bleeding, leukopenia in the body and neurological symptoms. It is distributed worldwide and is listed as a class A infectious disease by the International Veterinary Bureau. CSFV is a single-stranded positive-sense RNA virus of Flaviviridae and Pestivirus. The genome is about 12.3kb long, encoding 4 structural proteins and 7 non-structural proteins. The E0 protein, also known as Erns protein, is the first CSFV An envelope glycoprotei...

Claims

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