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Preparation method of magnetic functionalized electrochemical microfluidic sensor

An electrochemical and sensor technology, applied in the field of preparation of magnetic functionalized electrochemical microfluidic sensors, can solve the problems of high experimental cost, low sensitivity, long detection period, etc., and achieve enhanced catalytic performance, high sensitivity, and easy operation. Effect

Inactive Publication Date: 2021-08-13
UNIV OF JINAN
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  • Abstract
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Problems solved by technology

[0002] Lung cancer is the most common and deadly type of cancer with the highest frequency of diagnosis. Lung cancer is an important threat to human life. About 1.5 million people die from lung cancer every year; the increase of tumor marker levels can reflect the occurrence and development of cancer. Progress, providing a means of screening and diagnosis, some tumor markers have become important monitoring targets for adjuvant therapy of lung cancer, such as neuron-specific enolase; neuron-specific enolase (NSE) is a neuron-specific enolase It is an important specific marker of neuroendocrine tumors, such as neuroblastoma, medullary thyroid carcinoma and small cell lung cancer, etc. It can be used for differential diagnosis, disease monitoring, curative effect evaluation and Recurrence prediction; so far, neuron-specific enolase is usually measured by radioimmunoassay, enzyme-linked immunoassay, and fluorescent immunoassay, but these detection methods have complex operations, long detection cycles, low sensitivity, and experimental costs. High disadvantages; therefore the present invention has designed a novel electrochemical microfluidic sensor that is easy to operate, high in sensitivity and low in cost

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  • Preparation method of magnetic functionalized electrochemical microfluidic sensor

Examples

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Effect test

Embodiment 1

[0037] Example 1 Preparation of Magnetic Functionalized Electrochemical Microfluidic Sensor

[0038] (1) Preparation of ferric oxide

[0039]Add 2.7 g of ferric chloride hexahydrate and 7.2 g of sodium acetate into 100 mL of ethylene glycol solution, stir magnetically to obtain a uniform yellow solution, transfer the solution to a polytetrafluoro autoclave, and react at 200 °C 8 h, washed with ethanol for 5 times by magnetic separation, and dried under vacuum at 60 °C;

[0040] (2) Preparation of molybdenum disulfide coated with ferric oxide

[0041] Disperse 0.1 g of iron ferric oxide nanoparticles in 50 mL of ultrapure water under ultrasonic conditions, then add 0.066 g of ammonium molybdate and 0.143 g of thiourea, sonicate for 20 minutes, and seal the solution in a polytetrafluoro autoclave , heated at 180°C for 12 h, washed with ethanol five times after magnetic separation, and dried in vacuum at 60°C;

[0042] (3) Preparation of gold-coated platinum core-shell nanopar...

Embodiment 2

[0051] Example 2 Preparation of microfluidic chip

[0052] The microfluidic chip layer made of polydimethylsiloxane was prepared according to the designed pattern, the channel layer (2) and the microwell layer (3) were bonded by oxygen plasma treatment for 50 s, and the fixture layer was printed by a 3D printer , embedded in the groove (11) with a diameter of 3mm in the lower fixture (5), the fixture layer fixes the electrode layer (4) printed with the electrode system (10) and the microfluidic chip layer.

Embodiment 3

[0053] Example 3 Electrochemical microfluidic sensor for the detection of neuron-specific enolase

[0054] (1) Pass 20 µL of phosphate buffer solution with a pH value of 7.4 into the detection cell from the sample inlet;

[0055] (2) Connecting the three-electrode system (10) printed on the microfluidic chip to the electrochemical workstation;

[0056] (3) The electrochemical response of the standard solution of neuron-specific enolase at different concentrations was detected by chronoamperometry, and 5 mol / L was injected from the hydrogen peroxide inlet (7) at a flow rate of 10 μL / min when running for 60 s. Hydrogen peroxide solution, record the current intensity when running for 120 s, and draw the working curve;

[0057] (5) Use neuron-specific enolase solution of unknown concentration instead of neuron-specific enolase standard solution for detection, and calculate the content of neuron-specific enolase according to the obtained working curve.

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Abstract

The present invention relates to a preparation method of a magnetic functionalized electrochemical microfluidic sensor. Ferroferric oxide-coated molybdenum disulfide-gold-coated platinum is adopted as a substrate material, the excellent magnetism of the material is beneficial to loading on a magnetic working electrode of a micro-fluidic chip, and a foundation is laid for further construction of the sensor; and the synergistic catalysis effect of molybdenum disulfide with excellent electrocatalytic activity and the gold-coated platinum core-shell nanoparticles further enhances the electrochemical signal response, and the magnetic functionalized electrochemical microfluidic sensor constructed on the microfluidic chip can realize simple, rapid and sensitive detection of neuron-specific enolase.

Description

technical field [0001] The invention relates to the preparation of a magnetically functionalized electrochemical microfluidic sensor, and relates to the fields of novel functional materials, electrochemical sensors and microfluidic. Background technique [0002] Lung cancer is the most common and deadly type of cancer with the highest frequency of diagnosis. Lung cancer is an important threat to human life. About 1.5 million people die from lung cancer every year; the increase of tumor marker levels can reflect the occurrence and development of cancer. Progress, providing a means of screening and diagnosis, some tumor markers have become important monitoring targets for adjuvant therapy of lung cancer, such as neuron-specific enolase; neuron-specific enolase (NSE) is a neuron-specific enolase It is an important specific marker of neuroendocrine tumors, such as neuroblastoma, medullary thyroid carcinoma and small cell lung cancer, etc. It can be used for differential diagnosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 魏琴郭玉健代笠任祥马洪敏吴丹孙旭
Owner UNIV OF JINAN
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