A serum-free medium for culturing mesenchymal stem cells

Abstract

The invention relates to a serum-free medium for culturing mesenchymal stem cells. Based on the volume of the serum-free medium, it includes the following components: α-MEM 10.2g / L, sodium bicarbonate 2.4g / L, L-glutamine 1-5mM, poloxamer 18850-300mg / L , Recombinant Human Albumin 2‑8g / L, Recombinant Human Transferrin 10‑20mg / L, Recombinant Human Insulin 2‑10mg / L, Hepes1‑5mM, β‑Mercaptoethanol 50nM, Lipid 0.1‑1mg / L, Trace Element 1‑5mg / L, glutathione 0.1‑5mg / L, p-aminobenzoic acid 0.5‑5mg / L, hydrocortisone 1‑50ng / mL, vitamin PP20‑50mg / L, vitamin C5‑50mg / L , compound of formula I 2-10μM, compound of formula II 5-20μM, progesterone 10-20ng / mL, putrescine 1-10mg / L, heparin 1-10IU / mL, EGF1-10ng / mL, b-FGF1- 10ng / mL, HGF1‑10ng / mL, VEGF1‑10ng / mL. The MSCs serum-free medium BPS‑SFM is a chemically defined, animal-derived substance-free, serum-free medium; .

Description

technical field [0001] The invention relates to a serum-free medium for culturing mesenchymal stem cells, which belongs to the technical field of cell engineering and biomedicine. Background technique [0002] Mesenchymal stem cells (MSCs) are adult stem cells that widely exist in human tissues and organs. Because of their high self-renewal ability and multi-directional differentiation potential, they are used in clinical hematopoietic support, promotion of stem cell implantation and immune regulation. Has potential application prospects. At present, large-scale preclinical and clinical studies have confirmed that MSCs are directly infused as cell therapy products, or combined with scaffold materials to construct tissue engineered tissues and organs (skin, bone or cartilage, etc.) for transplantation. It has good safety and effectiveness in the treatment of burns, bone defects, soft tissue filling, end-stage liver disease, myocardial infarction, diabetes and other trauma or...

AI Technical Summary

Problems solved by technology

[0007] (3) High batch-to-batch variability makes it difficult to standardize the cell production process;

[0008] (4) Serum may also contain growth inhibitory factors and cytotoxic substances;

[0009] (5) It is very difficult to carry out quality control to reduce various pollution risks;

[0010] (6) The effects of other unknown factors, hormones and other substances are yet to be studied

Serum-free medium has the characteristics of clear components, and can well maintain the proliferation and differentiation pluripotency of MSCs in vitro, but there are problems such as high price and short storage time

At the same time, most of these media need auxiliary gelatin and other matrigel to coat the culture plate, which not only may introduce animal-derived components, but also increases the workload of cell culture expansion and the chance of contamination during passage

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