Bacterial inhibition type water purifier for marine aquaculture and preparation method thereof
A technology for marine aquatic products and purifiers, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of rare bacillus and unsuitable mariculture, and reduce the activation time , Efficient removal of nitrite nitrogen, and the effect of overcoming frequent epidemics
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] Example 1 Screening, isolation and identification of bacterial strains YB-01 and YB-02
[0034] 1. Preparation of culture medium:
[0035] Ammonia nitrogen degrading bacteria enrichment screening medium: (NH4) 2 SO 4 2g, NaH 2 PO 4 0.25g, MnSO 4 4H 2 O0.01g, K 2 HPO 4 0.75g, MgSO 4 ·7H 2 O 0.03g, CaCO 3 3g, aged sea water 1L, pH 7.5-8.0.
[0036] Solid medium for separation and purification of ammonia nitrogen degrading bacteria: (NH4) 2 SO 4 0.5g, NaH 2 PO 4 0.25g, MnSO 4 4H 2 O 0.01g, K 2 HPO 4 0.75g, MgSO 4 ·7H 2 O 0.03g, CaCO 3 2g, aged sea water 1L, agar powder 12g, pH 7.5-8.0.
[0037] Nitrite nitrogen degrading bacteria enrichment screening liquid medium: NaNO 2 1g, Na 2 CO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.03g, FeSO 4 ·7H 2 O 0.4g, aged sea water 1L, pH 7.2-7.5.
[0038] Solid medium for isolation and purification of nitrite nitrogen degrading bacteria: NaNO 2 0.5g, Na 2 CO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.03g, F...
Embodiment 2
[0050] Example 2 Physiological and biochemical characteristics of bacterial strains YB-01 and YB-02
[0051] YB-01 and YB-02 were detected according to the physiological and biochemical detection methods of the "Bergery Bacteria Identification Handbook" (Eighth Edition), and the detection indicators included sugar fermentation, alcohol fermentation, starch hydrolysis, lipase, methyl acetylphthalein, Catalase, casein hydrolysis, protein hydrolysis, citrate utilization, tyrosine hydrolysis, phenylalanine deamination and acid production, etc. The test results are shown in Table 1.
[0052] Table 1. Physiological and biochemical characteristics of YB-01 and YB-02 strains
[0053]
[0054] Note: In Table 1, means positive, and means negative.
Embodiment 3
[0055] Example 3 Performance testing of bacterial strains YB-01 and YB-02
[0056] (1) Detection of ammonia nitrogen and nitrite nitrogen removal rate: the national standard phenol hypochlorite colorimetric method and naphthalene ethylenediamine hydrochloride diazo azo colorimetric method (GB_17378.4-2007) were used to detect ammonia nitrogen and nitrite nitrogen respectively state nitrogen concentration. Under simulated seawater farming conditions (mainly salinity 32‰, pH 7.5, temperature 24-26, dissolved oxygen ≧5mg / L), the initial concentration of ammonia nitrogen of YB-01 or YB-02 is 5, 10, 25 and 50mg respectively. The 24-hour ammonia nitrogen removal rate of / L simulated solution; and the 24-hour nitrite nitrogen removal rate of the two strains to the simulated solution with initial concentration of nitrite nitrogen of 0.5, 2, 5 and 10mg / L, each concentration gradient Set up 3 repetitions. The preparation method of YB-01 or YB-02 for detection is: YB-01 or YB-02 probio...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com