Method for producing aerobic type single-cell protein through autolysis process
A single-cell protein and autolysis technology, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of not considering the decomposition of nucleic acids into high-value nucleotides, low protein digestibility, and difficult product control. , to achieve the effect of controllable residence time, fast temperature rise and easy temperature control
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Embodiment 1
[0054] Embodiment 1: the establishment of free nucleotide and free base detection method
[0055] Use free nucleotides and free bases as the main indicators for the adjustment of autolysis process parameters and the comparison of products before and after autolysis. The principle is: raw materials such as yeast and bacterial protein will contain free nucleotides and bases after autolysis or enzymatic hydrolysis, and the sample extracted from the aqueous solution will be analyzed by high-performance liquid chromatography. Salt is used as the mobile phase, and the external standard method can be used for quantitative determination of 5 nucleotides, 5 nucleosides and 5 bases.
[0056] Free nucleotides refer to the sum of 5 nucleotides and 5 nucleosides, including cytidylic acid, uridine acid, guanylic acid, inosinic acid, adenylic acid, cytidine, uridine, inosine, guanylic acid glycosides, adenosine. Free base refers to the sum of 5 nucleic acid bases, including cytosine, uraci...
Embodiment 2
[0121] Embodiment 2: autolysis production technology and product
[0122] The autolysis production process and products developed by the present invention are single-cell protein products made by using the cultivation technology of the invention patent WO2009059163A1, but are not limited thereto, and are also suitable for the processing of aerobic single-cell protein products similar to this invention, specifically Contains the following steps:
[0123] 1) Precipitation and concentration: regularly discharge the bacterial protein produced by the bioreactor into the sedimentation concentration tank through the pipeline, the bacterial protein is precipitated at the bottom of the conical concentration tank, and the moisture content of the bottom material is reduced to below 97%, and the subsequent dehydration process can be carried out .
[0124] a) The pool body diameter in this embodiment: 15 meters, depth: 3.5 meters, effective volume: 477 cubic meters.
[0125] 2) Use a pos...
Embodiment 3
[0158] Embodiment 3: digestibility test
[0159]The difference in apparent digestibility between the bacterial protein using the autolysis process and the common bacterial protein was evaluated by animal experiments.
[0160] Digestibility is the percentage of digestible nutrients in the feed ingredients ingested by animals. The indicator method is to use the indicator existing in the feed or artificially mixed evenly. After feeding the fish, the digestibility was calculated from the amount of the unit indicator contained in the unit feed and feces. Formulating formulas based on the digestible value of nutrients in feed ingredients is of great significance for improving the digestibility of compound feed and reducing the pollution of feed substances to the aquaculture water environment.
[0161] Test plan:
[0162] Raw materials for evaluation: The bacterial protein with a protein content of 50% was autolyzed according to the method of Example 2, and the same batch of singl...
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