Method for producing aerobic type single-cell protein through autolysis process

A single-cell protein and autolysis technology, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of not considering the decomposition of nucleic acids into high-value nucleotides, low protein digestibility, and difficult product control. , to achieve the effect of controllable residence time, fast temperature rise and easy temperature control

Pending Publication Date: 2015-09-09
TAIZHOU ICELL BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing process is directly dried and microwave sterilized after dehydration, which destroys most of the endogenous enzymes, so no active substances will be produced;
[0007] 3) The protein digestibility of the existing processing technology is still not high, and there is still room for further improvement;
[0008] 4) The single-cell protein has a relatively high content of nucleic acid. The drying treatment of the ordinary process does not decompose the nucleic acid into nucleotides and remains in the cell. However, decomposing the nucleic acid into nucleotides by a certain method can make the single-cell protein be applied to animals. Feed has two obvious effects of attracting food and immunity. The existing technology does not consider how to decompose nucleic acid into high-value nucleotides
However, these two patents are not suitable for this patent. The raw materials used in this patent application are anaerobic bacteria. The characteristics of bacteria are quite different from those of aerobic bacteria. The autolysis methods are also very different. Long, need to add enzymes to promote autolysis, anaerobic bacteria autolysis has more restrictions on the reaction vessel, moisture content, pH, temperature, etc., and the product is difficult to control, and it is easy to produce a high content of spoilage products

Method used

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  • Method for producing aerobic type single-cell protein through autolysis process
  • Method for producing aerobic type single-cell protein through autolysis process
  • Method for producing aerobic type single-cell protein through autolysis process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the establishment of free nucleotide and free base detection method

[0055] Use free nucleotides and free bases as the main indicators for the adjustment of autolysis process parameters and the comparison of products before and after autolysis. The principle is: raw materials such as yeast and bacterial protein will contain free nucleotides and bases after autolysis or enzymatic hydrolysis, and the sample extracted from the aqueous solution will be analyzed by high-performance liquid chromatography. Salt is used as the mobile phase, and the external standard method can be used for quantitative determination of 5 nucleotides, 5 nucleosides and 5 bases.

[0056] Free nucleotides refer to the sum of 5 nucleotides and 5 nucleosides, including cytidylic acid, uridine acid, guanylic acid, inosinic acid, adenylic acid, cytidine, uridine, inosine, guanylic acid glycosides, adenosine. Free base refers to the sum of 5 nucleic acid bases, including cytosine, uraci...

Embodiment 2

[0121] Embodiment 2: autolysis production technology and product

[0122] The autolysis production process and products developed by the present invention are single-cell protein products made by using the cultivation technology of the invention patent WO2009059163A1, but are not limited thereto, and are also suitable for the processing of aerobic single-cell protein products similar to this invention, specifically Contains the following steps:

[0123] 1) Precipitation and concentration: regularly discharge the bacterial protein produced by the bioreactor into the sedimentation concentration tank through the pipeline, the bacterial protein is precipitated at the bottom of the conical concentration tank, and the moisture content of the bottom material is reduced to below 97%, and the subsequent dehydration process can be carried out .

[0124] a) The pool body diameter in this embodiment: 15 meters, depth: 3.5 meters, effective volume: 477 cubic meters.

[0125] 2) Use a pos...

Embodiment 3

[0158] Embodiment 3: digestibility test

[0159]The difference in apparent digestibility between the bacterial protein using the autolysis process and the common bacterial protein was evaluated by animal experiments.

[0160] Digestibility is the percentage of digestible nutrients in the feed ingredients ingested by animals. The indicator method is to use the indicator existing in the feed or artificially mixed evenly. After feeding the fish, the digestibility was calculated from the amount of the unit indicator contained in the unit feed and feces. Formulating formulas based on the digestible value of nutrients in feed ingredients is of great significance for improving the digestibility of compound feed and reducing the pollution of feed substances to the aquaculture water environment.

[0161] Test plan:

[0162] Raw materials for evaluation: The bacterial protein with a protein content of 50% was autolyzed according to the method of Example 2, and the same batch of singl...

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Abstract

The invention relates to the technical field of single-cell protein processing, in particular to a method for producing aerobic type single-cell protein through an autolysis process. The method comprises the steps of settling concentration, centrifugal dewatering, drying, sterilization and smashing and packaging, wherein autolysis and drying are carried out on materials with the moisture content of 78 percent to 85 percent obtained after the step of centrifugal dewatering in sequence. The autolysis process is adopted in the process and the manufactured product, autolytic enzymes fully decompose macromolecule nutrients and cell walls in the autolysis process, and high-content free amino acid and free nucleotide can be generated through autolysis. Meanwhile, polysaccharide of the cell walls is decomposed, so that cells are permeable, it is guaranteed that active substances can flow out of the cells, and therefore the active substances can be directly absorbed and utilized by animals. Autolysis is carried out after centrifugal dewatering, and the high efficiency of autolysis can be ensured; the content of moisture needing to be removed in a drying process after autolysis can be reduced, and therefore drying cost is lowered.

Description

[technical field] [0001] The invention relates to the technical field of single-cell protein processing, in particular to a method for producing aerobic single-cell protein by using an autolysis process. [Background technique] [0002] Single cell protein (SCP for short), also known as microbial protein or bacterial protein, uses industrial wastewater, waste gas, natural gas, petroleum alkanes, agricultural and sideline processing products, and organic waste as a medium to cultivate yeast, non-pathogenic Single-celled organisms such as sexual bacteria, micro-bacteria, fungi, etc., and then made after purification and drying, it is an important protein source for the food industry and feed industry. Single-cell protein products are rich in protein, with a complete range of amino acids and appropriate proportions. Single-cell protein is also rich in fat, carbohydrates, nucleic acids, vitamins and inorganic salts, and contains calcium, phosphorus, potassium, iron, magnesium, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/06
CPCC12N1/066C07K1/14
Inventor 许建刚宋建华赵维韦张鑫杰
Owner TAIZHOU ICELL BIO TECH CO LTD
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