Method for extracting and purifying flavones and phenolic acids in medicinal material ixeris sonchifolia through metal ion chelation

A technology of metal ions and bitter dishes, which is applied in the field of medicine, can solve the problems of many steps, complicated process, and long extraction cycle, and achieve the effects of good fluidity, simplified extraction process steps, and shortened extraction cycle

Inactive Publication Date: 2016-07-06
TONGHUA HUAXIA PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a fast and simple metal ion chelation method for extracting and purifying flavonoids and phenolic acids in Kudiezi medicinal material, aiming at the disadv...

Method used

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  • Method for extracting and purifying flavones and phenolic acids in medicinal material ixeris sonchifolia through metal ion chelation
  • Method for extracting and purifying flavones and phenolic acids in medicinal material ixeris sonchifolia through metal ion chelation
  • Method for extracting and purifying flavones and phenolic acids in medicinal material ixeris sonchifolia through metal ion chelation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Detection of luteolin-7-O-β-D-glucuronide (LGU) and cichoric acid (CCA) in samples by HPLC-UV method.

[0018] High-performance liquid chromatography is used for detection, and the chromatographic equipment and chromatographic conditions are as follows: SHIMADZULC-2010A (autosampler, column thermostat, quaternary pump, UV detector, Class-VP operating system); chromatographic column: Ultimate? AQC- 18 columns (250mm×4.6mm, 5μm, Welch Materials Inc.); mobile phase: A: methanol-tetrahydrofuran (volume ratio = 15:1); B: water (1% formic acid), using a gradient elution program, 0-45min: 30%A; 45-52min: 80%A; 52-60min: 30%A; the flow rate is 1mL / min, the column temperature is 40°C, the detection wavelength is 348nm, and the injection volume is 10μL.

[0019] Calculate the establishment of the standard curve.

[0020] Accurately weigh an appropriate amount of LGU and CCA standard substances after vacuum drying, dissolve them with chromatographic methanol to obtain ...

Embodiment 2

[0027] Different metal ions Mg 2+ , Ca 2+ , Zn 2+ Purification process and results of flavonoids (represented by LGU) and phenolic acids (represented by CCA).

[0028] 1 kg of Kudiezi medicinal material was reflux extracted with 15 and 12 times the amount of 50% ethanol for 2 times, each time for 1 hour. The extracts are combined, the ethanol is recovered under reduced pressure until there is no alcohol smell, the obtained suspension is dried by spray drying, and the obtained product is collected and protected from moisture for later use. Accurately weigh an appropriate amount of dried Kudiezi extract, use methanol to prepare a solution with a concentration of 1mg / mL through ultrasonic oscillation, and filter for later use. Take Kudiezi filtrate 100ml, add 0.1mol / L metal salt (MgCl 2 , CaCl 2 , ZnCl 2 ) of methanol solution, and after fully shaking for 15 minutes, add dropwise 1mol / L NaOH solution to adjust the pH value of the solution. Stand for 4 hours, filter, and wa...

Embodiment 3

[0031] Fourier Transform Infrared Spectroscopy (FT-IR).

[0032] Weigh an appropriate amount of vacuum-dried samples, mix them with dry KBr, and press them into tablets. Use an infrared spectrometer (IFS-55, Bruker, Germany) to measure the Fourier infrared spectrum, scanning range: 4000-400cm -1 .

[0033] The infrared spectrum of Kudiezi ethanol 50% extract, Kudiezi extract magnesium ion precipitate (pH9) is as follows Figure 4 , 5 shown. There is a hydroxyl vibration peak formed around 3427. 1628-1629, 1384cm -1 Nearby are the asymmetric and symmetric stretching vibration peaks of the carbonyl group, 1264 and 1068cm -1 are the asymmetric and symmetric stretching vibration absorption peaks of the C-O-C group. 1115-1121cm -1 is the C-O absorption peak. The absorption peak range of MgO is 500-1000cm -1 , peak at 860cm -1 . Figure 5 Medium 1500cm -1 The enhancement of the nearby vibration peaks indicates that the content of aromatic ring substances has increased, t...

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Abstract

The invention belongs to technical the field of medicine and relates to the extraction of flavone and phenolic acid compounds from a medicinal material ixeris sonchifolia through a metal ion chelation process. A method for extracting and purifying flavones and phenolic acids in a medicinal material ixeris sonchifolia through metal ion chelation is characterized by comprising the following steps: (1) extraction; (2) solvent recovery and moisture removal; (3) dissolution in an organic solvent and filtering; (4) metal ion chelation; and (5) filtering and drying to obtain the product. The feature that flavones and phenolic acids often have o-diphenol hydroxyl or adjacent hydroxyl and carbonyl structures and can be chelated with metal ions is conducive to the chelation reaction in a certain alkalinity range, and the chelate can be precipitated in the organic solvent and separated out. The extraction technology of ixeris sonchifolia flavones is simplified, the extraction period is shortened, the technology is simple and easy to understand, and the production cost can be reduced; and moreover, the product has stable quality, loose property and good fluidity and facilitates further preparation processing.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to extracting flavonoids and phenolic acid compounds from Kudiezi (Brassicia chinensis) medical material by using a metal ion chelation method. Background technique [0002] The traditional Chinese medicine Kudiezi is the dried whole plant of Ixerissonchifolia (Bge) Hance, a plant of the genus Asteraceae. Kudiezi contains active ingredients such as flavonoids, phenolic acids and sesquiterpenes. Kudiezi injection refined by modern technology is clinically used to treat coronary heart disease, stroke, myocardial infarction and cerebrovascular disease. Studies have shown that Kudiezi flavonoids and phenolic acids have a good protective effect on cardiovascular. The flavonoids in Kudiezi mainly include glycosides with luteolin and apigenin as aglycones, mainly luteolin-7-O-β-D-glucuronide (Luteolin-7-O-β -D-glucuronide, LGU), Luteolin-7-O-β-D-glucoside (Luteolin-7-O-β-D-glucoside), Lut...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K8/97A61P9/00A61P9/10A61P25/00C07H17/07C07H1/00C07C69/732C07C67/48A23L33/105A61K8/9789
CPCA61K36/28A23V2002/00A61K8/97A61K9/2018A61K2236/33A61K2236/331A61K2236/333A61K2236/51C07C67/48C07H1/00C07H17/07C07C69/732A23V2250/2116A23V2250/21A23V2300/14
Inventor 吴春福刘东春邹敬韬王芳王东杨静玉张文颖郭真真陈峰林芳屈延乐代英辉
Owner TONGHUA HUAXIA PHARMA
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