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Lignocellulose-degrading composite microbial system and culture method and application thereof
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A technology of lignocellulose and composite bacteria, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve problems such as low degradation speed and single microorganisms
Inactive Publication Date: 2020-03-31
陕西山河生物科技有限公司
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[0006] In order to solve the technical problem that the existing lignocellulose biodegradation process is mainly aimed at a single microorganism and has a low degradation rate, the present invention uses rotten straw as a new biological resource to provide a lignocellulose-degrading composite strain and its cultivation Methods and Applications
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Embodiment 1
[0131] This embodiment mainly involves the screening of highly efficient biomass cellulose degrading bacteria, and the construction of a composite bacterial system, mainly including:
[0135] Accurately weigh 10.0 g of the straw sample, add it into a 250 mL Erlenmeyer flask containing 90 mL of sterile saline and glass beads, shake it on a constant temperature shaker at 37 ° C, and mix the sample thoroughly for 3 hours for later use.
[0137] a) Dilute the collected samples step by step 10 times with sterile water to 10 -6 、10 -7 and 10 -8 After doubling, take 0.1mL and spread it on the cellulose Congo red selective medium, repeat the coating on 3 plates for each concentration, culture at 28°C, observe regularly, and pick the strains with clear transparent circles around the colonies;
[0138] b) Purify the picked strains by st...
Embodiment 2
[0209] In this example, the complex bacterial strain constructed by the high-efficiency biomass cellulose degrading bacteria screened out in Example 1 is used to prepare the complex bacterial enzyme preparation, and the process flow is as follows Figure 11 As shown, it mainly includes the process of strain cultivation, fermentation process, extraction, separation and purification of enzymes, finished enzymes, testing and packaging.
[0212] The compound strains of selected fungi, bacteria and actinomycetes are amplified and cultured on the corresponding medium. Specific steps:
[0213] 1.1) Clean the inclined surface with sterile physiological saline, shake well, put it into a sterile flask and shake well;
[0214] 1.2) Inoculate the oblique culture in the seed bottle, cultivate the bacteria at 30°C and 180r / rain for 12 hours to obtain the seed liquid, and cultivate the mold at 28°C and 220r / rain for 48 hours t...
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Abstract
The invention provides a lignocellulose-degrading composite microbial system and a culture method and an application thereof, and solves problems that an existing lignocellulose biodegradation processmainly aims at a single microbe and has a low degradation speed. The composite microbial system includes trichoderma, penicillium, clostridium, bacillus and herbaspirillum, wherein the trichoderma, the penicillium, the clostridium, the bacillus and the herbaspirillum respectively have a mass percent of 20%-40%, 20%-40%, 5%-10%, 20%-40% and 5%-10%; a composite microbial culture medium for culturing the composite microbial system is a cellulosecongo red culture medium; and a fermentation culture medium using the composite microbial system includes the following components: 5% of wheat bran, 20% of maize strawpowder, 0.05% of yeast extract, 0.3% of peptone, 0.03% of magnesiumsulfate heptahydrate, 0.4% of potassium dihydrogen phosphate, 0.03% of calciumchloride dihydrate, 1.5% of ammoniumsulfate, 0.8% of tween 80, and the balance being water. The composite microbial system has an application in straw degradation.
Description
technical field [0001] The invention relates to the compound bacterial agent technology, in particular to a lignocellulose-degrading compound bacterial strain and its cultivation method and application. Background technique [0002] In recent years, the global food crisis, energy crisis, and environmental crisis have become increasingly serious. People have gradually turned their attention to the most abundant and cheapest lignocellulose resources on the earth. Lignocellulose is a mixture of cellulose, hemicellulose, and lignin. , is the largest renewable resource on earth. Microbial degradation and conversion of lignocellulose is an important part of the natural carbon cycle. If this resource is effectively utilized, it will have far-reaching significance in solving the energy shortage and environmental pollution problems facing the world today. [0003] Since about 80% of corn stover components are cellulose, hemicellulose, and lignin, and lignin that is difficult to degr...
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