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Bacillus subtilis pro1a2, its bacterial agent and preparation method and its application in melon cultivation

A technology of Bacillus subtilis and melon, which is applied in the field of biological control, can solve the problems of difficult promotion and implementation, uneven distribution, etc., achieve good results, improve the ecological environment, and be easy to use

Active Publication Date: 2022-04-15
兰考县搏洋农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Crop rotation is also an effective way to prevent or reduce pests and diseases, but it is difficult to popularize and implement in the case of limited per capita arable land and uneven geographical distribution in my country.

Method used

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  • Bacillus subtilis pro1a2, its bacterial agent and preparation method and its application in melon cultivation
  • Bacillus subtilis pro1a2, its bacterial agent and preparation method and its application in melon cultivation
  • Bacillus subtilis pro1a2, its bacterial agent and preparation method and its application in melon cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and screening of Bacillus subtilis

[0030] (1) Isolation and purification of soil Bacillus

[0031] Collection (collected by Liang Shen in Dinggedai Village, Sanyizhai Township, Lankao County on September 20, 2016) 300g of moist tillage soil 5-20cm from the surface layer, weighed 10g after crushing, and placed in a 100ml In the Erlenmeyer flask of bacterial water, shake for 3 to 5 minutes, and heat in a water bath at 80°C for 10 minutes. Measure the soil suspension and dilute with sterile water to prepare 10 -2 , 10 -3 , 10 -4 and 10 -5 Soil suspension; suck up the above 10 -3 , 10 -4 , 10 -5 50 μl of soil suspension was spread on NA medium (beef extract 3g, yeast powder 1g, peptone 5g, glucose 10g, agar 12g, water 1L, pH 6.8~7.0, sterilized at 121℃ for 20min) plate, in ultra-clean Plates were coated on a bench and allowed to dry. Inverted in a biochemical incubator and incubated at 37 °C for 48 h. After the single spore colony has grown,...

Embodiment 2

[0052] Example 2: Identification of Bacillus subtilis Pro1A2

[0053] (1) Analysis of physical and chemical properties

[0054] 1) Colony characteristics and bacterial morphology observation

[0055] Bacillus Pro1A2 was inoculated on NA medium and cultured in a biochemical incubator at 37 °C for 48 h. The colony morphology and growth of the strain on the medium were observed. Bacillus colonies cultured for 48 h were picked and Gram-stained on glass slides to observe the size and morphology of the bacteria.

[0056] 2) Physiological and biochemical test of strain Pro1A2 strain was measured by the medium and experimental methods recommended in "System Identification Manual of Common Bacteria" and "Bacillus spp.". Physiological and biochemical tests mainly include: methyl red reaction, contact enzyme test, starch hydrolysis test, hydrogen peroxide test, gelatin liquefaction test, casein decomposition test, anaerobic growth test, indole reaction, cellulose degradation test, gro...

Embodiment 3

[0071] Example 3: Preparation of microbial inoculum

[0072] The preparation steps of Pro1A2 inoculum are as follows:

[0073] (1) Strain culture and preparation

[0074] Inoculate Bacillus subtilis Pro1A2 into a test tube slanted soybean casein solid medium (tryptone 15g, soybean protease hydrolyzate 5g, sodium chloride 5g, glucose 2.5g, agar powder 12g, add water to make up to 1000ml, pH 7.0~ 7.5, after packaging, sterilize at 121°C for 20min), and cultivate at 28-35°C for 24-36 hours to obtain bacterial cells;

[0075] (2) Preparation of seed solution

[0076] The test tube strain prepared in step (1) was inserted into the soybean casein liquid medium (tryptone 15g, soybean protease hydrolyzate 5g, sodium chloride 5g, glucose 2.5g, and water was added to make up to 1000ml, pH 7.0-7.5 , after packaging, sterilize at 121°C for 20min), shake and culture at 28-35°C for 12-16 hours to make seed liquid;

[0077] (3) Preparation of fermentation medium

[0078] Glucose, corn f...

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Abstract

The invention discloses a bacillus subtilis Pro1A2, its bacterial agent, a preparation method and its application in melon cultivation, aiming at solving the technical problems of poor disease control effect and insurmountable continuous cropping obstacles in melon cultivation. A Bacillus subtilis Pro1A2 was screened out for the first time in the present invention, and it was preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO. M 2017194. The microbial agent prepared by Bacillus subtilis Pro1A2 can not only have a good control effect on plant fungal diseases such as muskmelon blight, muskmelon wilt, wheat take-all, rice blast, etc., but also has a good effect on the control of bacterial diseases, such as It has a strong inhibitory effect on melon bacterial fruit spot, peach bacterial perforator, tobacco bacterial wilt, cabbage soft rot and tomato canker; it can also overcome the obstacles of continuous cropping of melon premature senescence; the control is efficient, easy to use, and No environmental pollution.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a kind of Bacillus subtilis ( Bacillus subtilis ) Pro1A2, its bacterial agent and preparation method and its application in muskmelon cultivation. Background technique [0002] Common diseases of melon include fruit spot, blight, fusarium wilt, powdery mildew, downy mildew, sclerotinia, angular spot, anthracnose, etc., and continuous cropping aggravates the occurrence of such diseases and continuous cropping obstacles. , if effective control is not carried out in time, it will seriously affect the yield and quality of melon. [0003] In the prior art, various measures can be taken to prevent and control diseases, such as plant quarantine, utilization of disease-resistant varieties, crop rotation, agricultural control, chemical control, physical control, and biological control. [0004] Disease-resistant varieties are the most simple, economical and effective preven...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/125
CPCC12N1/20A01N63/22C12R2001/125C12N1/205
Inventor 梁慎赵卫星李晓慧常高正康利允高宁宁徐小利王龙飞杨作运
Owner 兰考县搏洋农业发展有限公司
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