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Marine fungus-bacterium symbiont, metabolite of marine fungus-bacterium symbiont and application of metabolite in preparation of antibacterial drugs

A technology of symbionts and fungi, applied in the direction of chemicals for biological control, antibacterial drugs, and methods based on microorganisms, can solve the problems of no medicine, severe environment, residues, etc., and achieve good antibacterial activity and The effect of antifungal activity, simple process and reliable source

Active Publication Date: 2021-04-23
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, vancomycin-resistant Enterococcus (VRE), multi-drug-resistant Mycobacterium tuberculosis (MDRMT), and the newly emerged NDM-1-producing bacteria called "super bacteria" in recent years The emergence of drug-resistant bacteria (New Delhi-metallo-lactamase, New Delhi metallo-lactamase), let us face the embarrassing situation that once infected, there is no cure
Frustratingly, since the 1980s, due to the increasing difficulty of obtaining new antibacterial drugs through high-throughput screening of potential drug targets, the speed of research and development of new antibiotics is far from meeting people's needs. Antibiotics are particularly urgent
[0003] In addition, with the excessive use of organic chemical pesticides, not only serious environmental pollution and pesticide residues have been brought about, but also the emergence of drug resistance of some common plant pathogenic fungi

Method used

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  • Marine fungus-bacterium symbiont, metabolite of marine fungus-bacterium symbiont and application of metabolite in preparation of antibacterial drugs
  • Marine fungus-bacterium symbiont, metabolite of marine fungus-bacterium symbiont and application of metabolite in preparation of antibacterial drugs
  • Marine fungus-bacterium symbiont, metabolite of marine fungus-bacterium symbiont and application of metabolite in preparation of antibacterial drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Separation and purification of bacterial strains

[0032] Take an appropriate amount of seabed silt from Daya Bay in the northern part of the South my country Sea, put it in a clean petri dish, and air-dry it for later use (about a week). Put appropriate amount of air-dried sea mud and fresh sea mud into a 250mL Erlenmeyer flask filled with 30mL of artificial seawater (a small amount of glass beads can be added inside), shake at 200rpm on a shaker at 28°C for 30min, and then heat-shock in a water bath at 55°C. 5min standby. Use a pipette gun to draw 350 μL of the prepared sea mud sample, and evenly spread it on the plate of the prepared fucose-proline agar. The formula of the medium is: proline 1g, fucose-proline 5g,K 2 HPO 4 1g, MgSO 4 .7H 2 O 1g, (NH 4 ) 2 SO 4 1g, multivitamin (p-aminobenzoic acid, riboflavin, folic acid, inositol, pantothenic acid, Vc, VB1, VB6, VB12 50mg each, biotin 25mg), sea salt 30g, CaCO 3 2g, 20g of agar powder, add wa...

Embodiment 2

[0033] Embodiment 2: the identification of bacterial strain

[0034] 1. Morphological identification

[0035] The obtained culture host fungus F190 (fungus / bacteria symbiont Spiromastix sp.SCSIO F190 / Alcaligenes faecalis SCSIO B001) bacteria cake was inoculated onto the poured ISP2, ISP4 and MEA plates, cultured in the dark at 28°C, and the growth morphology of the colonies was observed regularly every day . The sporulation structure and the morphology of the spores were observed with an optical microscope and a scanning electron microscope, and the size of the spores was measured. Its ecological characteristics are as figure 2 As shown, the colony grows faster on the ISP2 medium, and the diameter of the colony is 1.0-3.5mm in 3 days under the dark condition of 28°C. The colony is white at the beginning, and then gradually turns orange. Such as figure 2 A-D); The growth of the colony on the ISP4 medium is slow, and the diameter of the colony is smaller for 3 days at 28°C...

Embodiment 3

[0042] Embodiment 3: Preparation of the fermentation broth of fungi / bacteria symbiont Spiromastix sp.SCSIO F190 / Alcaligenes faecalisSCSIO B001

[0043] Pick an appropriate amount of fungal / bacterial symbiont Spiromastix sp.SCSIOF190 / Alcaligenes faecalis SCSIO B001 from the prepared ISP2 medium plate and add it to 50mL of PDB medium, culture at 28°C and 200r / min on a shaker for 2-3 days Obtain seed liquid. Then carry out expanded fermentation, add all 50mL seed liquid into a 1L Erlenmeyer flask containing 200mL PDB medium, culture on a shaker at 28°C for 7 days at 200r / min to collect the fermentation liquid, centrifuge the fermentation liquid at 3900r / min to get the supernatant , that is, the strain fermentation broth is used for subsequent use.

[0044] Remarks: The PDB medium used above is the potato dextrose water medium purchased from Huankai Biotechnology Co., Ltd.

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Abstract

The invention discloses a marine fungus and bacterium symbiont, a metabolite of the marine fungus and bacterium symbiont and application of the marine fungus and bacterium symbiont in preparation of antibacterial drugs or biopesticides. The fungus / bacterium symbiont Spiromastix sp.SCSIO F190 / Alcaligenes faecalis SCSIO B001 is screened out from seabed sediments of the Daya Bay in the north of the south China Sea and has the preservation number of GDMCC 60747. According to the invention, the compound spiromarmycin is separated from the fermentation broth butanone extract of the fungus / bacterium symbiont, and the biological activity test results of the compound show that the compound spiromarmycin has good antibacterial activity and good antifungal activity, and can be used for developing novel antibacterial agents and antifungal agents so as to prepare the related novel antibacterial agents.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fungi-bacteria symbiont Spiromastix sp.SCSIO F190 / Alcaligenes faecalis SCSIO B001 derived from Daya Bay and its metabolite spiromarmycin and its application in the preparation of antibacterial drugs or biological pesticides. Background technique [0002] In recent years, various types of drug-resistant pathogenic bacteria have rapidly emerged and spread, such as methicillin-resistant Staphylococcus aureus (MRSA), which has high drug resistance and strong pathogenicity, causing systemic infection and mortality Can be as high as 50% or more. At present, MRSA has developed resistance to all β-lactam antibacterial drugs, aminoglycosides, quinolones, and macrolide antibacterial drugs. The only clinically effective drugs are mainly glycopeptide antibacterial drugs, such as known as Vancomycin is the "last line of defense for mankind". However, vancomycin-resistant Enterococ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P17/18C07D493/04A01N43/90A61K31/37A61P31/10A61P31/04A01P3/00A01P1/00C12R1/05C12R1/645
CPCC12P39/00C12P17/181C07D493/04A01N43/90A61K31/37A61P31/10A61P31/04
Inventor 鞠建华邵明伟孙长利李青连王晓雪
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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