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Application of gene TaPT16 in improving resistance of plants to powdery mildew

A technology of powdery mildew, gene, applied in the field of agricultural biology

Active Publication Date: 2021-09-28
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Previous research on phosphorus transporters has mainly focused on the role of plants in the uptake and redistribution of inorganic phosphate, and the role of phosphorus transporters in plant defense has rarely been reported.

Method used

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  • Application of gene TaPT16 in improving resistance of plants to powdery mildew
  • Application of gene TaPT16 in improving resistance of plants to powdery mildew
  • Application of gene TaPT16 in improving resistance of plants to powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Mycorrhizal and low phosphorus treatment

[0025] The wheat at the three-leaf stage was transplanted into the medium of fine sand, vermiculite and perlite mixed with two kinds of mycorrhizae of Glomus mosesi and Glomus terrestriale respectively, and the inoculation amount of mycorrhizae was 200 spores / 15g. Grown in a light incubator with a photoperiod of 16h / 8h and a temperature of 18°C / 15°C, all inoculated and control plants were watered twice a week with 1 / 2 Hoagland nutrient solution, 5 μM KH 2 PO 4 Low phosphorus treatment, 500μM KH 2 PO 4 For high phosphorus treatment, after 6 weeks, the wheat roots were taken, washed with water, and the root samples were harvested, frozen in liquid nitrogen and stored at -80°C for later use.

[0026] Total RNA was extracted from the mycorrhizae and the samples treated with low phosphorus and high phosphorus using TRIzol Reagent (invitrogen).

[0027] Using 1 μg of total RNA as a template, the first-strand cDNA was synthesized...

Embodiment 2

[0030] Example 2 Analysis of resistance to wheat powdery mildew by overexpressing TaPT16 in wheat

[0031] In order to further analyze the function of TaPT16, the full-length 1671bp of TaPT16 was cloned and constructed into the plant vector IPK003 promoted by the maize ubiquitin promoter to obtain the overexpression recombinant vector IPK003-TaPT16, and the recombinant vector and empty vector were transformed into Agrobacterium by heat shock method GV3101 was transformed into Zhoumai 26 through the Agrobacterium-mediated transformation method, and 5 single-copy homozygous transgenic lines were obtained after screening, and they were inoculated simultaneously on the leaves of wild type Zhoumai 26 and 5 transgenic plants at the three-leaf stage Fresh powdery mildew spores of wheat were placed on a 1% agar plate added with 85 μM benzimidazole, and the plate was placed in a climate incubator at 20°C to incubate for 4 hours. / cm 2 ) Gently shake the fresh conidia of powdery mildew...

Embodiment 3

[0037] Example 3 Analysis of the Resistance of Overexpressing TaPT16 in Wheat to Wheat Stem Rot

[0038] In order to further analyze the function of TaPT16 in the interaction between wheat and other pathogenic bacteria, the pathogenicity of wheat stalk rot was analyzed in 5 single-copy homozygous transgenic plants of TaPT16. Firstly, inoculate the dominant pathogen of wheat stalk rot - Fusarium graminearum block on the potato glucose medium which is stored in -80°C refrigerator, and cultivate it in the incubator at 25°C for 3-5 days, and select the pseudomonas with better growth. 4-6 pieces of Fusarium graminearum in 100mL CMC sporulation medium (carboxymethyl cellulose 15g / L, ammonium nitrate 1g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate heptahydrate 0.5g / L , yeast extract 1g / L, autoclaved at 121°C for 20min), cultured on a shaker at 25°C at 180rpm for 5-6 days; filtered with three layers of gauze in an ultra-clean bench to remove the bacteria cake, poured into...

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Abstract

The invention belongs to the field of agricultural biology, and particularly relates to application of a gene TaPT16 in improving the resistance of plants to powdery mildew. The arbuscular mycorrhiza-induced phosphorus transporter gene TaPT16 can be used for improving the disease resistance of plants, and has the nucleotide sequence as shown in SEQ ID NO: 1. The TaPT16 gene is determined to be the arbuscular mycorrhiza-induced phosphorus transporter gene, the disease resistance of a plant can be enhanced after the seedling of the TaPT16 gene over-expressed homozygous plant is inoculated with powdery mildew, the result is consistent with that of seedling when the TaPT16 gene over-expressed homozygous plant wheat in the mature period is inoculated with the powdery mildew by field and pot planting, and the disease resistance of the plant to powdery mildew. Therefore, the TaPT16 gene can be used for improving the resistance of wheat to powdery mildew, and has wide application space in plant breeding and cultivation.

Description

technical field [0001] The invention belongs to the field of agricultural biology, and in particular relates to the application of gene TaPT16 in improving plant resistance to powdery mildew. Background technique [0002] Phosphorus is the second largest element necessary for plant growth and development. It is widely involved in energy transfer, signal transduction, biosynthesis of macromolecules, photosynthesis, regulation of respiration and other metabolic processes, and plays an important role in plant growth. Phosphorus exists mainly in the form of complexes, insolubles and organic forms in soil, while plants mainly obtain phosphorus in the form of inorganic phosphorus, so phosphorus in soil is difficult to be absorbed and utilized by plants. Plants have evolved a series of effective strategies to promote phosphorus uptake and utilization in response to the stress of low phosphorus environment, including changing root system structure, root system secretion of organic a...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/46
CPCC07K14/415C12N15/8282Y02A40/146
Inventor 张怡李成伟李俐俐徐克东
Owner ZHOUKOU NORMAL UNIV
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