The utility model belongs to the technical field of breeding, and particularly relates to an epidemic prevention isolation device which comprises a loading component capable of enabling the device to be easily moved to a required position, and a plurality of isolation components capable of forming non-intercommunicated isolation areas to respectively retain diagnosed and suspected infected animals are arranged in front of and behind the loading component. An air exhaust
assembly capable of limiting the flowing direction of air in the isolation assemblies is arranged between the isolation assemblies. According to the utility model, a plurality of physical isolation spaces are conveniently constructed in the frame through the isolation assemblies which are not in contact with each other, and the air exhaust assemblies limit the flow direction of air in the isolation assemblies, and the
HEPA filters capable of intercepting pathogens are arranged at the
tail ends of the air flow paths in the isolation assemblies; the animals can be isolated under the conditions that the animals do not make contact with one another and
pathogen transmission is limited, and therefore on the basis that concentrated isolation of multiple animals is achieved, suspected infected animals are effectively prevented from being confirmed due to
continuous exposure.