The invention discloses a medical resource integration system and an establishment and use method thereof. The medical resource integration system comprises a main service module, a doctor use module, a diagnosis and treatment point use module, a patient use module, a memorizer, a network port, a print device, a scanning device and an input device, wherein the main service module comprises an analysis module, and the patient use module comprises an appointment register module, a doctor and patient interaction module, an inspection item inquiring module, a prescription inquiring module, a rehabilitation module and a payment module. The medical resource integration system relatively separates doctors from a diagnosis and treatment point, achieves online diagnosis and treatment, strengthens doctor and patient interaction, saves a large amount of time for the doctors and patients, avoids a crowded hospital environment, fully integrates and uses the existing doctors and existing diagnosis and treatment devices, and facilitates balanced, healthy and sustainable development of the medical field.