The invention provides a method of identifying day age of cephalopods through beaks and includes a method of grinding a beak longitudinal section and counting growth striation. The method mainly includes the steps of: extraction, cleaning, soaking, cutting, arrangement, resin embedding, cutting, immobilization on a glass slide, grinding and polishing, picture forming, and growth striation counting. In the method, the beak longitudinal section is ground respectively from the left and right sides to a core surface towards a central line. A prepared section is photographed by CCD to ensure that the growth striation can be read at any time in future. The growth striation is counted from the rear end to the front end of the beak in a direction being vertical to the growth striation all the time, thereby ensuring counting accuracy. The method solves the problems that the beak is difficult to grind and is liable to break during grinding, and the growth striation is difficult to observe and count.