Cysteine/ferroferric oxide/copper sulfide/BSA (bovine serum albumin) nano-composite particle as well as preparation and application thereof

A technology of nanocomposite particles and bovine serum albumin, which is applied in the direction of non-active components of polymer compounds, wave energy or particle radiation treatment materials, drug combinations, etc., can solve problems such as obvious side effects of the human body, damage to normal tissues, and poor water solubility. Achieve the effects of shortening the preparation cycle, good biocompatibility, and low cost

Inactive Publication Date: 2017-05-31
SHANGHAI UNIV OF ENG SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] High doses of chemotherapy drugs have obvious side effects on the human body, and non-specific drug delivery can cause damage to normal tissues
Paclitaxel ("PTX"), a drug with good anti-cancer effect, is extremely poor in water solubility, and the existing clinical dosage form (dissolved in polyoxyethylene castor oil and absolute ethanol at a ratio of 1:1) has also been proved that the solvent castor Sesame oil can cause serious side effects on normal tissues and cells

Method used

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  • Cysteine/ferroferric oxide/copper sulfide/BSA (bovine serum albumin) nano-composite particle as well as preparation and application thereof
  • Cysteine/ferroferric oxide/copper sulfide/BSA (bovine serum albumin) nano-composite particle as well as preparation and application thereof
  • Cysteine/ferroferric oxide/copper sulfide/BSA (bovine serum albumin) nano-composite particle as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A kind of cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particle, with BSA binding cysteine ​​to modify Fe 3 o 4 , CuS nanocrystals are the shell, and the inner space is used to load anticancer drugs (such as PTX) that are poorly soluble in water. The loading ratio of the nanocomposite particles to PTX is 5%-9%.

[0043] The preparation method of the cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particles comprises the following steps:

[0044] (1) Magnetic Fe 3 o 4 The particles are prepared according to the traditional co-precipitation method: 1.4g of FeCl 2 4H 2 O and 2.0 g FeCl 3 ·6H 2 O was added with 100 mL of deionized water, stirred vigorously under the protection of nitrogen and heated to 50°C, then quickly added 30 mL of ammonia water, and the solution was heated to 80°C and kept for 2.5 hours. Then it was cooled to room temperature, and a black precipitate was obtained by adding an external magnet, w...

Embodiment 2

[0059] A kind of cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particle, described nanocomposite particle is made of Cys, Fe 3 o 4 , CuS and BSA form a shell structure, in which Cys modifies Fe 3 o 4 Afterwards, CuS is combined with BSA to form a shell through disulfide bond, CuS is adsorbed with BSA, and the space in the shell is used to load the anticancer drug PTX which is poorly soluble in water, and the diameter of the nanocomposite particles is about 300nm.

[0060] The preparation method of the above-mentioned cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particles comprises the following steps:

[0061] (1) Take Fe 3 o 4 Ultrasonic dispersion of nanoparticles in PBS (0.02M, pH6.0), then adding cysteine ​​aqueous solution, ultrasonication, then adding carbodiimide hydrochloride, keeping in a water bath at room temperature, stirring, centrifuging, and washing, to obtain Cys- Fe3 o 4 , dispersed in deionized water ...

Embodiment 3

[0074] A kind of cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particle, described nanocomposite particle is made of Cys, Fe 3 o 4 , CuS and BSA form a shell structure, in which Cys modifies Fe 3 o 4 Afterwards, CuS is combined with BSA to form a shell through disulfide bond, CuS is adsorbed with BSA, and the space in the shell is used to load the anticancer drug PTX which is poorly soluble in water, and the diameter of the nanocomposite particles is about 300nm.

[0075] The preparation method of the above-mentioned cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particles comprises the following steps:

[0076] (1) Take Fe 3 o 4 Ultrasonic dispersion of nanoparticles in PBS (0.02M, pH6.0), then adding cysteine ​​aqueous solution, ultrasonication, then adding carbodiimide hydrochloride, keeping in a water bath at room temperature, stirring, centrifuging, and washing, to obtain Cys- Fe 3 o 4 , dispersed in deionized wate...

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Abstract

The invention relates to a cysteine/ferroferric oxide/copper sulfide/BSA (bovine serum albumin) nano-composite particle as well as preparation and application thereof. The nano-composite particle is degraded by utilizing ultrasonic waves with comparatively high power on a water/oil mixed solution interface to form microemulsion; Fe3O4 modified by cysteine is combined with BSA by a disulfide bond to form a shell; CuS is adsorbed on the BSA by electrostatic interaction; a comparatively large internal space is used for loading hydrophobic drug PTX (paclitaxel, dissolved in polyoxyethylated castor oil) insoluble in water. Compared with the prior art, the nano-composite particle has wide application prospect in the aspects of nuclear magnetic imaging, targeted drug delivery, photothermal therapy, therapy combined with chemotherapy and the like, an anti-cancer drug and a photothermal reagent can be transmitted to cancer positions, the toxic and side effects on normal tissues and cells are reduced, meanwhile, cancer cells are effectively killed, so that the treatment effect is further improved; a preparation method is simpler (a one-step microemulsion method is adopted) and has the advantage of large-scale production.

Description

technical field [0001] The invention relates to the technical field of hydrophobic anticancer drug carriers, in particular to a cysteine / ferric oxide / copper sulfide / bovine serum albumin nanocomposite particle and its preparation and application. Background technique [0002] High doses of chemotherapy drugs have obvious side effects on the human body, and non-specific drug delivery can cause damage to normal tissues. Paclitaxel ("PTX"), a drug with good anti-cancer effect, is extremely poor in water solubility, and the existing clinical dosage form (dissolved in polyoxyethylene castor oil and absolute ethanol at a ratio of 1:1) has also been proved that the solvent castor Sesame oil can cause serious side effects on normal tissues and cells. The development of nanotechnology, especially the application of nanocarriers in the field of biomedicine, has achieved leapfrog development, which has given birth to the development of a new field of "nanomedicine". Therefore, in orde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/18A61K47/02A61K41/00A61K31/337A61P35/00A61K49/14A61K49/10A61K49/08A61K49/18
CPCA61K31/337A61K41/0052A61K47/02A61K47/183A61K47/42A61K49/08A61K49/10A61K49/143A61K49/18
Inventor 任兰芳刘锡建陆杰闫大伟黄智马国昌樊学学吴书平
Owner SHANGHAI UNIV OF ENG SCI
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