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A double-layer porous nerve guide with directional guidance function and its preparation method

A nerve conduit and guiding function technology, applied in the field of double-layer porous nerve conduit and its preparation, can solve problems such as poor toughness, poor material cell affinity, difficult conduit configuration nerve regeneration, etc., to prevent invasion and repair peripheral Long-segment nerve defect and the effect of inhibiting the formation of neuroma

Active Publication Date: 2019-09-10
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although some biodegradable nerve guides have been put into the market, there are still problems such as poor cell affinity and poor toughness of materials, and it is difficult to have a good catheter configuration to guide nerve regeneration and repair long-segment defects.

Method used

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  • A double-layer porous nerve guide with directional guidance function and its preparation method
  • A double-layer porous nerve guide with directional guidance function and its preparation method
  • A double-layer porous nerve guide with directional guidance function and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A double-layer porous nerve guide with directional guidance function, its preparation method comprises the following steps:

[0042] 1) Prepare the outer film: Dissolve 1 g of polylactic acid-glycolic acid copolymer (PLGA) in 15 ml of dichloromethane, add 6 g of sodium chloride particles with a particle size of 10 μm after complete dissolution, and pour into polystyrene after ultrasonic dispersion is complete. In a tetrafluoroethylene groove mold, air-dried for 5 days to form, then soaked in deionized water for 2 days to remove sodium chloride, and vacuum-dried for 2 days to remove organic solvent to obtain the outer film.

[0043] 2) Preparation of chitosan (CS-RGD) grafted with RGD: 0.1g (0.176mmol) biological polypeptide GRGDY was dissolved in 20mL of sodium acetate-acetic acid buffer solution (mass fraction 1%), and 0.677g of condensation activator was added (3.530mmol) 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 0.406g (3.530mmol) N-hydroxy...

Embodiment 2

[0057] A double-layer porous nerve guide with directional guidance function, its preparation method comprises the following steps:

[0058] 1) Prepare the outer film: Dissolve 1 g of polylactic acid-glycolic acid copolymer (PLGA) in 15 ml of dichloromethane, add 6 g of sodium chloride particles with a particle size of 50 μm after complete dissolution, and pour into polystyrene after ultrasonic dispersion is complete. In a tetrafluoroethylene groove mold, air-dried for 5 days to form, then soaked in deionized water for 2 days to remove sodium chloride, and vacuum-dried for 2 days to remove organic solvent to obtain the outer film.

[0059] 2) Preparation of chitosan (CS-RGD) grafted with RGD: 0.1g (0.176mmol) biological polypeptide GRGDY was dissolved in 20mL of sodium acetate-acetic acid buffer solution (mass fraction 1%), and 0.677g of condensation activator was added (3.530mmol) 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 0.406g (3.530mmol) N-hydroxy...

Embodiment 3

[0067] A double-layer porous nerve guide with directional guidance function, its preparation method comprises the following steps:

[0068] 1) Prepare the outer film: dissolve 1g of polyethylene lactide-ethylene glycol block copolymer (PLGA-MPEG) in 15ml of dichloromethane, and add 6g of sodium chloride particles with a particle size of 30 μm after completely dissolving After the ultrasonic dispersion is complete, pour it into a polytetrafluoroethylene groove mold, air-dry for 5 days to form, then soak in deionized water for 2 days to remove sodium chloride, and vacuum dry for 2 days to remove the organic solvent to obtain the outer film.

[0069] 2) Preparation of chitosan grafted with RGD (CS-RGD): 0.1 g (0.161 mmol) of biological polypeptide c-RGDyK was dissolved in 20 mL of sodium acetate-acetic acid buffer solution (mass fraction 1%), and a condensation activator was added 0.618g (3.227mmol) 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 0.371g (3.22...

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Abstract

The invention discloses a double-layered porous nerve conduit with a directional guidance function and a preparation method thereof. The double-layered porous nerve conduit is characterized in that an RGD-grafted modified natural biological macromolecule and calcium-phosphorus-nanoparticle-doped electrospun nanofiber film serves as an inner layer, and a porous film prepared from degradable polyester serves as an outer layer. The preparation method comprises the following steps: (1) preparing the outer-layer film with longitudinal grooves and a porous structure; (2) RGD bio-polypeptides are grafted into natural biological macromolecules, calcium-phosphorus nanoparticles are doped to prepare a spinning solution, and the spinning solution is formed on the inner wall, with the longitudinal groove structure, of the outer-layer film, through electrospinning to form the inner-layer nanofiber film; and (3) rolling the double-layered film by a mandrel to prepare a conduit. The double-layered porous nerve conduit has favorable biocompatibility and cellular affinity, can promote the growth of nerve cells and improve the nerve regeneration 'microenvironment', has a favorable mechanical support, can prevent in-growth of scar tissues, guarantees transmission of nutrient substances, realizes directional guidance of nerve regeneration, and is suitable for regeneration and repair of peripheral nervous defects.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a double-layer porous nerve guide with directional guidance function and a preparation method thereof. Background technique [0002] In recent years, more than 1 million people in the world suffer from peripheral nerve injury every year. Due to various factors such as complex pathological process and slow nerve regeneration after peripheral nerve injury, the functional recovery of the injured nerve is restricted. At present, peripheral nerve injury is mainly treated clinically by stump anastomosis and nerve transplantation. The stump anastomosis is difficult to achieve accurate anastomosis of nerve bundles, which affects the regeneration speed of nerve fibers, and the existence of sutures will cause further damage and neuroma. However, the gold standard - autologous nerve transplantation has insurmountable defects such as limited sources, permanent loss of donor ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/06A61L27/40A61L27/56A61L27/58A61L27/50A61L27/18A61L27/20A61L27/22A61L27/12
Inventor 戴红莲程乔李俊云陈瑀哲徐黛云
Owner WUHAN UNIV OF TECH
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