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Fluorescent probe for quantitatively detecting vitamin B12 based on carbon quantum dot fluorescence quenching method as well as preparation method and application thereof

A technology of fluorescence quenching and carbon quantum dots, which is applied in the field of fluorescent probes, can solve the problems of weak anti-interference ability, cumbersome steps, and high cost, and achieve the effects of strong anti-interference ability, simple preparation method, and stable performance

Active Publication Date: 2019-03-12
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have their own unique advantages, but all have the following defects: cumbersome steps, time-consuming and laborious, complicated and expensive instruments, long detection cycle, high technical content, high cost, weak anti-interference ability and low accuracy, etc. The method cannot be widely used

Method used

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  • Fluorescent probe for quantitatively detecting vitamin B12 based on carbon quantum dot fluorescence quenching method as well as preparation method and application thereof
  • Fluorescent probe for quantitatively detecting vitamin B12 based on carbon quantum dot fluorescence quenching method as well as preparation method and application thereof
  • Fluorescent probe for quantitatively detecting vitamin B12 based on carbon quantum dot fluorescence quenching method as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Preparation and characterization of PNCQDs

[0023] Step 1: Weigh 0.4 g of sucrose, add 6 mL of ethylenediamine and 4 mL of concentrated phosphoric acid to it, and obtain a dark brown viscous substance.

[0024] Step 2: After the beaker was cooled to room temperature, 30 mL of secondary water was added to it, stirred, centrifuged at 8000 rpm for 15 minutes, and dialyzed with a 500-1000 Da dialysis bag for three days to obtain a clear PNCQDs solution.

[0025] Step 3, freeze-drying to obtain solid powder of PNCQDs.

[0026] Step 4: Weigh 0.5 g of PNCQDs solid powder into a beaker, add 50 mL of secondary water to it, stir to fully dissolve it, and obtain a PNCQDs stock solution with a concentration of 10 mg / mL.

[0027] Characterization see figure 1 with figure 2 . figure 1 In the ultraviolet absorption spectrum of PNCQDs, there are two obvious absorption peaks located at 276 nm and 323 nm, which are determined by n→π of C=O * Surface defects caused by...

Embodiment 2

[0028] Example 2: Vitamin B 12 Detection anti-jamming experiment

[0029] Step 1, weighing different mass metal ions (Fe 3+ , Pb 2+ , K + , Fe 2+, Mg 2+ , Cu 2+ , Al 3+ , Ni 2+ , Ag + , Ca 2+ , Cr 3+ , Na + , Mn 2+ , Zn 2+ ) compound, add 10 mL of secondary water to prepare a metal ion stock solution with a concentration of 0.1 mol / L.

[0030] Step 2, weighing a certain mass of cysteine, tryptophan, aspartic acid, lysine, threonine, alanine, valine, tyrosine, glutamic acid, phenylalanine , asparagine, glycine, histidine, isoleucine, methionine, L-leucine, and serine, and add 5 mL of secondary water to prepare an amino acid stock solution with a concentration of 0.01 mol / L.

[0031] Step 3, weigh 0.015 g VB respectively 1 , 0.0062 g VB 3 , 0.0122 g VB 7 , add 5 mL of normal saline, stir to dissolve, and prepare 0.01 mol / L B vitamin stock solution.

[0032] Step 4: Add 9 µL of metal ion stock solution to the carbon dot solution (2 mL) with a concentration of 2...

Embodiment 3

[0036] Example 3: Vitamin B 12 Linear equation for titrating PNCQDs

[0037] Step 1, measure the fluorescence intensity of 2 mg / mL carbon dot solution, denoted as F 0 .

[0038] Step 2, add VB drop by drop 12 For the stock solution, record the fluorescence intensity respectively, denoted as F. Changes in fluorescence intensity see Figure 5 .

[0039] Step 3, use Origin software to fit the change of fluorescence intensity ( F 0 / F ) and VB 12 Concentration between the linear equation, the results see Image 6 .

[0040] Figure 5 shows that with VB 12 With the addition of the stock solution, the fluorescence of PNCQDs was gradually quenched, indicating that VB 12 It has a specific quenching effect on the fluorescence of PNCQDs. Image 6 is the change value of fluorescence intensity of PNCQDs and VB 12 The graph of the linear relationship between concentrations, the linear equation is F 0 / F = 0.11956[VB 12 ] + 1.35303, the linear range is 1.99-31.01 µmol / L,...

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Abstract

The invention belongs to the technical field of fluorescent probes, and provides a fluorescent probe for quantitatively detecting the vitamin B12 based on a carbon quantum dot fluorescence quenching method as well as a preparation method and application thereof. Saccharose serves as a carbon source, ethidene diamine serves as a nitrogen source, concentrated phosphoric acid serves as a phosphorus source, PNCQDs is prepared through an acid-based neutralization heat releasing carbonization method, undissolved substance is removed through centrifugalization, unreacted precursor substance and micromolecules are removed through dialysis, and fluorescent probe PNCQDs solid powder is obtained through freeze-drying; and the linear relation between the vitamin B12 concentration and PNCQDs fluorescence intensity is determined by utilizing a fluorescence detection method. The content of the vitamin B12 in the practical sample is detected through a marking recycling experiment. The method is easy and convenient to operate, the anti-interference capability is high, expensive instrument and equipment are not needed, the detection cost is low, and the content of the vitamin B12 in the practical sample can be rapidly, efficiently and quantitatively detected.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, in particular to a method for quantitatively detecting vitamin B based on carbon quantum dot fluorescence quenching method 12 Fluorescent probes and their preparation methods and applications. Background technique [0002] Vitamin B 12 (Vitamin B 12 , VB 12 ), also known as cobalamin or cyanocobalamin, is a B vitamin composed of cobalt-containing porphyrin compounds. VB 12 It is also the only vitamin that contains essential minerals. It is red because of the cobalt content. It is also called red vitamin. When an adult lacks VB 12 Sometimes it can cause pernicious anemia, inflammation of the tongue, oral cavity, and digestive tract mucous membranes, degeneration of the spinal cord, and degeneration of nerves and peripheral nerves; children lack VB 12 The early manifestations of mental and emotional abnormalities, sluggish expression, less crying and fussing, unresponsiveness, lov...

Claims

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Application Information

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IPC IPC(8): C09K11/65G01N21/64
CPCG01N21/6428C09K11/65G01N2021/6432
Inventor 弓晓娟张俐董文娟宋胜梅董川
Owner SHANXI UNIV
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