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Production method of disease-resistant deinsectization yield-increasing organic fertilizer and application thereof

A production method and technology of organic fertilizer, applied in the direction of organic fertilizer, application, fertilization device, etc., can solve the problems of unusable cigarette production, plant disease infection, virus repeated infection, etc., achieve the prevention of physiological diseases, reduce the amount of chemical fertilizer, Eliminates the effect of soil compaction

Inactive Publication Date: 2020-06-05
云南土宝宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Organic fertilizer is mainly processed from organic solid waste, turning waste into treasure. At present, the raw materials used in the production of organic fertilizer include straw, livestock and poultry manure, agricultural by-products and solid waste from food processing. China has the largest tobacco planting area in the world. In China, the annual output of tobacco leaves is 4.5-5 million tons, of which about 25% of leftovers such as tobacco powder and tobacco stems are discarded and cannot be used for cigarette production. The current treatment method is direct burning and burying, causing environmental pollution. pollution, and tobacco powder itself is rich in a large amount of nitrogen, phosphorus, potassium and trace elements. At the same time, it also has the effect of killing insects and repelling insects. It is an ideal raw material for making organic fertilizers. However, tobacco virus disease is more serious. Tobacco virus There are 16 kinds of diseases, among which the viruses that cause tobacco mosaic disease include TMV, CMV, and PVY. Direct use of tobacco dust as organic fertilizer raw materials is likely to cause disease infection of crops and cause losses. This is why tobacco dust has never been used. One of the reasons why it can be used in large quantities in the production of organic fertilizers is that if it can be effectively treated to avoid virus infection in tobacco dust, applying tobacco dust to the production of organic fertilizers can not only increase the production of organic fertilizers, but also effectively solve the problem of Now the problem of tobacco powder being discarded, wasting and polluting the environment is a topic worth studying
Because tobacco leaves contain components such as nicotine that are not conducive to the growth of microorganisms, traditional HM decomposing agents cannot decompose tobacco leaves well. If the harmless treatment is not thorough, these pathogenic bacteria will be brought back to the soil, causing the virus to repeat. Infect

Method used

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  • Production method of disease-resistant deinsectization yield-increasing organic fertilizer and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A method for producing an organic fertilizer that resists diseases, eliminates insects, and increases production, is carried out according to the following steps:

[0040](1) Pretreatment: crush the tobacco rods and the destroyed tobacco stems separately to obtain 60-80 mesh tobacco powder; control the moisture content of cow dung below 75%, and remove the obvious impurities in the feces; break the waste bacteria bag , control the particle size within 3cm, and remove the plastic film in the crushed raw materials;

[0041] (2) The raw materials are mixed according to the following parts by weight: 20-40 parts of cow dung, 10-30 parts of destroyed tobacco stem powder, 20-35 parts of humic acid, 10-20 parts of waste bacteria bag, 15 parts of tobacco rod powder -20 parts, stir well to mix;

[0042] (3) Main fermentation: prepare by adding 1 kilogram of compound fermentation bacterial agent for every 10 tons of raw material mixture, control the moisture content to 52-65%, a...

Embodiment 2

[0051] The preparation of compound fermentation agent:

[0052] ①Preparation of culture medium for each strain of compound fermentation agent:

[0053] PDA medium: 200g potatoes, add 1000ml water and boil for half an hour, filter through gauze, add 10-20g glucose and 17-20g agar, sterilize with high-pressure steam (121°C) for 20 minutes.

[0054] LB medium: tryptone 10g, yeast extract 5g, sodium chloride (NaCl) 10g, add deionized water to 1000mL, pH=7.0, sterilize by high-pressure steam (121°C) for 21 minutes;

[0055] MRS medium: tryptone 10g, beef extract powder 5g, yeast extract powder 4g, glucose 20g, Tween-80 1.08g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, triammonium citrate 2g, magnesium sulfate (MgSO4.7H2O ) 0.2g, manganese sulfate (MnSO4.4H2O) 0.05g, agar 15g, add distilled water to 1000mL, final pH 6.2±0.2, sterilize by high pressure steam (121℃) for 15 minutes.

[0056] ②Activation and purification of a single strain and expanded culture on a liquid s...

Embodiment 3

[0066] Medium: medium: lactose 21.57g; compound nitrogen source 2.6g, which contains peptone and ammonium sulfate, the mass ratio of the two is 1:3; manganese sulfate 0.21g; dipotassium hydrogen phosphate 1.5g; magnesium sulfate heptahydrate 1.2g ; Add deionized water to 1000mL, pH = 7.0, and sterilize with high-pressure steam (121°C) for 21 minutes;

[0067] Inoculate the seeds of Bacillus mucilaginosus Krassilnikov into the above-mentioned medium, shake and culture at 30°C, 170r / min for 24h, and transfer to a new medium with 5% (v / v) inoculation amount at 30°C, 170r / min Shake culture for 12 hours to the logarithmic phase to obtain seed liquid.

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Abstract

The invention discloses a production method of a disease-resistant deinsectization yield-increasing organic fertilizer. The organic fertilizer is prepared by pretreatment and mixing of raw materials,preparation of a composite fermentation inoculant, main fermentation, pile turning, after-ripening fermentation, preparation and addition of a functional inoculant and addition of quick lime; the organic matter content of the organic fertilizer is required to reach 50-70%, the total nutrient of N, P and K is greater than or equal to 5%, the fecal coliform number is negative, and the roundworm eggdeath rate is 100%. By using the organic fertilizer as a base fertilizer and applying the base fertilizer in holes, the yield increase and high harvest of potatoes, chilies, leaf lettuces, tomatoes, summer yellow and white, apples, grapes and strawberries are promoted. According to the invention, growth and propagation of harmful bacteria can be effectively inhibited, the soil environment is purified, crop root systems are protected, and soil-borne diseases are reduced; meanwhile, the organic fertilizer integrates phosphorus, potassium and trace element activation, so that nutrients are absorbed and utilized, the fertility is improved, the fertilizer efficiency is prolonged, and crops can achieve high yield and high efficiency by the production method and application of the disease-resistant deinsectization yield-increasing organic fertilizer.

Description

technical field [0001] The invention belongs to the technical field of organic fertilizer processing, and in particular relates to a production method and application of an organic fertilizer that resists diseases, eliminates insects, and increases production. Background technique [0002] Organic fertilizer is mainly processed from organic solid waste, turning waste into treasure. At present, the raw materials used in the production of organic fertilizer include straw, livestock and poultry manure, agricultural by-products and solid waste from food processing. China has the largest tobacco planting area in the world. In China, the annual output of tobacco leaves is 4.5-5 million tons, of which about 25% of leftovers such as tobacco powder and tobacco stems are discarded and cannot be used for cigarette production. The current treatment method is direct burning and burying, causing environmental pollution. pollution, and tobacco powder itself is rich in a large amount of nit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/40C05G3/60C05G3/80
CPCC05F3/00C05G3/00
Inventor 赵云祥
Owner 云南土宝宝生物科技有限公司
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