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Application of blumea balsamifera leaf total flavone

A technology of the total flavonoids of the leaves and the flavonoids of the leaves of Aina, which is applied in the field of the total flavonoids of the leaves of Aina, to achieve the effects of reducing the expression, increasing the water content, reducing epidermal hyperplasia and the generation of sunburn cells

Inactive Publication Date: 2020-09-18
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of total flavonoids in the leaves of Aina japonica in ultraviolet radiation, mainly for the treatment and repair of skin photodamage induced by UVB radiation

Method used

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  • Application of blumea balsamifera leaf total flavone
  • Application of blumea balsamifera leaf total flavone
  • Application of blumea balsamifera leaf total flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1-a kind of preparation method of total flavonoids of leaves of Aina radix, comprises the steps:

[0026] (1) Weigh 400 g of Aina japonica leaf powder passed through a 40-mesh sieve, add 4000 mL of ethanol solution with a mass concentration of 75-85% and cold-soak for 24 hours;

[0027] (2) Heating to reflux at 75-85°C for 1 hour, rotary steaming until alcohol-free, adding distilled water to dissolve, and obtaining a crude extract;

[0028] (3) After the crude extract is extracted with petroleum ether, it is purified by polyamide resin, concentrated under reduced pressure, and evaporated to dryness to obtain an extract;

[0029] (4) The extract was dissolved in an ethanol solution with a mass concentration of 85%, and the total flavonoids of the leaves of Aina japonica with a concentration of 100 mg / mL and 200 mg / mL were prepared respectively.

[0030] In order to confirm the effect of the total flavonoids of the leaves of Ainaspermia on skin photodamage tre...

Embodiment 2

[0031] Example 2-The effect experiment of the total flavonoids of Ai Naxiang leaves on the photodamage of mouse skin caused by UVB radiation

[0032] 1. Experimental method

[0033] (1) Experimental animals and groups

[0034] A total of 120 SPF-grade healthy female Balb / C mice (4-6 weeks), weighing 18-22 g, were provided by the Hubei Experimental Animal Research Center (production license: SCXK (E) 2015-0018).

[0035]Animals were kept in separate cages according to the standard conditions of clean animals, and adaptively fed for 7 days. Afterwards, they were randomly divided into 6 groups according to body weight, 20 animals in each group, which were blank control group (Normal Control, NC) and UVB radiation model group (UVB-irradiation, UVB), 85% ethanol solvent control group (Vehicle Control, VC), compound menthol ointment positive control group (PositiveControl, PC), the 100mg / mL Ainaxiang leaf total flavonoids treatment group (Total Flavonoids 1 , TFE1), the 200mg / mL t...

Embodiment 3

[0070] Example 3-The effect of total flavonoids from the leaves of Aina japonica on oxidative stress and DNA damage in the skin of mice with UVB radiation-induced photodamage

[0071] 1. Experimental method

[0072] Take 100 mg of tissue pieces from each group (take the skin from the sunburned model on the back of the mice after cervical dislocation) and rinse them in ice-cold saline to remove the blood on the tissue pieces, dry them with filter paper, weigh them, and use ophthalmic scissors as soon as possible. Cut up the tissue block, and then prepare it with an internal cutting tissue homogenizer (homogenization time 10 seconds / time, interval 30s, continuous 3 to 5 times, in ice water; prepare 10% homogenate with low temperature and low speed centrifugation Centrifuge at about 3500r / min for 6-8min, leave the supernatant of the centrifuged homogenate and discard the sediment below, and store at -80°C. The mouse 8-OHdG ELISA kit was purchased from Beijing Xinfang Biotechnolog...

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Abstract

The invention provides an application of blumea balsamifera leaf total flavone, and belongs to the technical field of medicines and cosmetics. The application of the blumea balsamifera leaf total flavone is an application of the blumea balsamifera leaf total flavone in medicines and cosmetics for treating and repairing skin light damage caused by ultraviolet radiation. Experimental results prove that the blumea balsamifera leaf total flavone can obviously relieve skin erythema and edema caused by UVB radiation, reduce epidermal hyperplasia and sunburned cell generation, shorten wound eschar removal time and increase the skin tissue water content; the activity levels of SOD and GSH are improved, the MDA content is reduced, and the 8-OHdG level is reduced to inhibit oxidative damage and DNAdamage; and through down-regulation of an NF-[kappa]B signaling pathway and the TNF-alpha level, severe epidermatitis is inhibited, the expression of p53 and PCNA is reduced, and the obvious treatmentand repairing effects on UVB radiation induced skin photodamage are achieved.

Description

technical field [0001] The invention relates to the technical field of pharmaceuticals and cosmetics, in particular to the use of total flavonoids from the leaves of Aina japonica. Background technique [0002] Human life is inseparable from the ultraviolet radiation (ultraviolet radiation, UVR) in sunlight. Ultraviolet rays (UV) in sunlight are the main environmental factors causing skin sunburn, aging and tumors. Excessive UVR can cause the increase of active oxygen groups in the cells, and the skin will appear erythema, edema, inflammation, abnormal hyperplasia, pigmentation and other skin sunburn manifestations, and in severe cases, it will promote the formation of blood clots in the human body, leading to premature aging, inducing heart disease, heart and brain. Various malignancies such as vascular disease and skin cancer. [0003] According to the wavelength of ultraviolet rays and different biological effects, the skin science community divides them into three band...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P17/16A61K8/9789A61Q19/00A61Q17/04A61K127/00
CPCA61K36/28A61Q17/04A61Q19/004A61K8/9789A61P17/16
Inventor 王丹于福来胡璇李小婷谢小丽王凯庞玉新
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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