Mesoporous silicon nanoparticles capable of responding to X-rays to release drugs as well as preparation method and application of mesoporous silicon nanoparticles

A nanoparticle and mesoporous silicon technology, applied in the field of mesoporous silicon nanoparticles and their preparation, can solve the problems of insufficient controllable drug release and slow degradation of mesoporous silicon, achieve efficient and safe tumor treatment, reduce toxic and side effects, and improve the The effect of antitumor effect

Inactive Publication Date: 2020-12-11
SOUTH CHINA UNIV OF TECH
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of slow degradation of existing mesoporous silicon and uncontrollable drug release, the primary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesoporous silicon nanoparticles capable of responding to X-rays to release drugs as well as preparation method and application of mesoporous silicon nanoparticles
  • Mesoporous silicon nanoparticles capable of responding to X-rays to release drugs as well as preparation method and application of mesoporous silicon nanoparticles
  • Mesoporous silicon nanoparticles capable of responding to X-rays to release drugs as well as preparation method and application of mesoporous silicon nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Preparation of dual-selenium mesoporous silicon (MON) nanoparticles that can respond to X-ray drug release

[0065] A method for preparing mesoporous silicon nanoparticles capable of responding to X-ray drug release, comprising the steps of:

[0066] (1) Preparation of bis[3-(triethoxysilyl)propyl]diselenide

[0067] Add 10 g of γ-chloropropyltrimethoxysilane dropwise to 30 mL of sodium diselenide solution (containing 0.0255 mol of sodium diselenide), stir overnight at room temperature, add ice water to stop the reaction, extract with dichloromethane, and use Dry over anhydrous sodium sulfate, and purify the crude product by silica gel column (300-400 mesh) chromatography (petroleum ether (PE): dichloromethane (DCM) = 10-1:1) to obtain a dark yellow liquid which is bis[3-( Triethoxysilyl)propyl]diselenide;

[0068] (2) Preparation of Diselenium Mesoporous Silicon (MON) Nanoparticles Responsive to X-ray Drug Release

[0069] Take 0.6g of cationic template (...

Embodiment 2

[0071] Example 2: Degradation of double selenium mesoporous silicon nanoparticles

[0072] After 100 μg of double-selenide mesoporous silicon nanoparticles (i.e., the double-selenide mesoporous silicon nanoparticles obtained in Example 1) were irradiated with 1 Gy of X-rays (irradiation time was 1 minute), H was added with a final concentration of 100 μM. 2 o 2 The aqueous solution was incubated at a constant temperature of 37°C at 200rpm for 72 hours, and samples were collected after 0 hour of X-ray irradiation (that is, the dual selenium mesoporous silicon nanoparticles obtained in Example 1), 1Gy of X-ray irradiation, and 72 hours of incubation. TEM detection;

[0073] Through transmission electron microscopy, it was found that the dual-selenium mesoporous silicon nanoparticles can rapidly degrade the dual-selenium mesoporous silicon (MON) nanoparticles after being irradiated with low-dose X-rays. The results are as follows: image 3 as shown in a, and then continue to sl...

Embodiment 3

[0074] Example 3: Mesoporous silicon-loaded doxorubicin and release of DOX

[0075] (1) MON@DOX drug loading and DOX release

[0076] Dissolve 50 mg of doxorubicin (DOX) in 50 mL of water to obtain a stock solution of 1 mg / mL. Suspend 50 mg of mesoporous silicon carrier in the prepared 50 mL DOX solution under ultrasound, shake the resulting mixture at 37 °C and 500 rpm for 12 hours, and centrifuge to obtain a solid, which is the drug-loaded dual selenium mesoporous silicon nanoparticles (MON @DOX), dried under vacuum at 30°C for later use. The drug loading was analyzed by UV-Vis spectroscopy at 480nm, the supernatant was collected, and the drug loading was calculated using the following formula:

[0077]

[0078] In the drug release test, the drug-loaded nano-drug delivery system (MON@DOX) was placed in 0 μM H 2 o 2 , 100 μM H 2 o 2 The PBS buffer solution and MON@DOX were put into 100 μM H 2 o 2 In the PBS buffer solution (0.01M pH7.4), shake at 37°C and 100rpm, t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Specific surface areaaaaaaaaaaa
Apertureaaaaaaaaaa
Login to view more

Abstract

The invention discloses mesoporous silicon nanoparticles capable of responding to X-rays to release drugs as well as a preparation method and application of the mesoporous silicon nanoparticles, and belongs to the technical field of drug carriers. The preparation method of the mesoporous silicon nanoparticles capable of responding to X-rays to release drugs comprises the following steps: (1) preparing bis[3-(triethoxysilyl)propyl]diselenide; and (2) preparing double-selenium mesoporous silicon nanoparticles capable of responding to X-rays to release drugs. The prepared double-selenium mesoporous silicon is the first mesoporous silicon capable of responding to X-rays to generate skeleton degradation. Bis[3-(triethoxysilyl)propyl]diselenide and tetraethoxysilane are used as a mixed silicon source, the mesoporous silicon containing double-selenium bonds is synthesized by a copolycondensation method, and the prepared double-selenium mesoporous silicon can not only respond to X-rays to rapidly degradate and release drugs, but also respond to tumor redox microenvironments to slowly release drugs so as to regulate the drug degradation speed and the drug release speed.

Description

technical field [0001] The invention belongs to the technical field of drug carriers, and in particular relates to a mesoporous silicon nanoparticle capable of responding to X-ray drug release, a preparation method and application thereof. Background technique [0002] Cancer has gradually become a major disease that threatens people's lives. Chemotherapy is currently an effective treatment, but due to its severe side effects and poor prognosis, it reduces the quality of life of patients. Advances in nanotechnology have provided new materials and new strategies for efficient and safe cancer chemotherapy. Nanomaterials have become the mainstay of cancer chemotherapy because of their good tumor targeting and easy combination of multiple treatments, which can improve treatment efficiency and reduce side effects. hotspot. Among them, mesoporous silicon nanoparticles have the advantages of large specific surface area and pore volume, good biocompatibility, easy surface modificat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/51A61K41/00A61K47/04A61K45/00A61P35/00B82Y5/00B82Y25/00B82Y30/00B82Y40/00A61K31/704
CPCA61K9/0009A61K9/5115A61K31/704A61K41/00A61K45/00A61P35/00B82Y5/00B82Y25/00B82Y30/00B82Y40/00
Inventor 邵丹杨超陈方满张帆董文飞王政
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap