50 results about "Chromosome 4" patented technology

The invention belongs to the technical field of molecular marker assisted breeding, particularly relates to a molecular marker related to white spot resistance on a corn chromosome 4 and application, and particularly provides the molecular marker related to the corn white spot resistance, the gene of the molecular marker is a Zm00001eb168510 gene and / or a sequence as shown in SEQ ID NO: 1, the CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO: 2, and the CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO: 1. According to the invention, through many years of multi-site experiments, the white spot resistance QTL qMWS4-4 interval and the resistance SNP: 4-14146725 and the associated gene Zm00001eb168510 thereof are jointly localized on the corn chromosome 4, the SNP locus can be localized in different environments through QTL linkage analysis and GWAS association analysis, and 15.14% of phenotypic variation can be explained; the associated candidate functional gene mainly encodes cysteine protease superfamily protein, and a cysteine protease large family contains deubiquitination enzyme which plays an important role in plant development and stress response.

The invention discloses an application of a TaAP2-4 gene in regulating and controlling starch content of wheat grains, which is used for enhancing the expression quantity of the TaAP2-4 gene to improve the starch content of the wheat grains and weakening the expression quantity of the TaAP2-4 gene to reduce the starch content of the wheat grains. The TaAP2-4 gene is located on a chromosome 4 of a wheat genome, and three copies, namely TaAP2-4-A, TaAP2-4-B and TaAP2-4-D, are formed on the chromosome 4. According to the invention, a Crispr / Cas9 gene editing vector for knocking out the TaAP2-4 gene is constructed by adopting a reverse genetics method, the TaAP2-4 gene is successfully knocked out in a wheat material by adopting an agrobacterium-mediated method, and the starch content of a knocked-out mutant is reduced, so that the condition that the TaAP2-4 gene plays a positive regulation role in a wheat grain starch synthesis process is known. In practical application, directional knockout mutation of the genome level TaAP2-4 gene can be achieved through a gene transformation process, the gene knockout mutation method can be used for verifying functions of the TaAP2-4 gene, and materials are provided for mining other starch synthesis related genes.

The invention belongs to the technical field of molecular marker assisted breeding, and particularly relates to a fresh sweet corn aphid resistance related SNP site combination and application thereof. The SNP (Single Nucleotide Polymorphism) site combination of the aphid-resistant fresh sweet corn comprises an S4174685152 positioned on a chromosome 4, and an S5207733210 and an S5207734430 positioned on a chromosome 5. The developed aphid-resistant functional breeding marker site combination for the fresh-eating sweet corn can assist in breeding of the fresh-eating corn, rapid detection and early detection are achieved, the resistance of the fresh-eating sweet corn to the corn aphid can be predicted only by detecting the genotype of a specific site, and the aphid-resistant functional breeding marker site combination has the advantages of being clear in selection target, easy and convenient to operate and not affected by the environment. The invention provides an effective way for efficient breeding of fresh sweet corn.

The application provides application of a SNP genetic marker affecting chicken body weight at the onset of lay in genetic breeding of laying hens, and belongs to the field of animal genetic breeding and biotechnology.The SNP genetic marker affecting chicken body weight at the onset of lay comprises bw1egg_1 and / or bw1egg_2; the Ensembl number of the bw1egg_1 is rs318020581, corresponding to the 76311052th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 3rd intron of the gene C1QTNF7, and the base at the position is T or G; the Ensembl number of the bw1egg_2 is rs313708699, corresponding to the 74731889th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 8th intron of the gene SLIT2, and the base at the position is T or C.Both the bw1egg_1 and the bw1egg_2 are helpful to genetically improve the body weight at the onset of lay, and when applied to genetic breeding of chickens, are favorable to improving the body weight at the onset of lay of the laying hens and obtaining a laying hen breed with excellent performance in uniformity of the body weight at the onset of lay.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

This invention discloses a SNP molecular marker associated with the growth traits of *Bellamya pear-shaped* and its application, belonging to the field of marker-assisted breeding technology. The SNP molecular marker is located at loci 131,293,660 on chromosome 4 of *Bellamya pear-shaped* (T>C variant), where the T allele (genotypes T / T and T / C) is significantly positively correlated with growth rate and body weight. This invention integrates genome-wide association analysis (GWAS) with an XGBoost machine learning model to screen key loci and designs specific primer pairs to achieve accurate genotyping. Population validation shows that individuals carrying the T allele have significantly higher growth rates and average body weights than individuals with the C / C genotype. This marker can be used for early prediction of growth performance in *Bellamya pear-shaped*, selection of high-yielding varieties, and construction of genetic maps, overcoming the bottlenecks of long breeding cycles and low efficiency in traditional breeding methods.

The application discloses a molecular marker for distinguishing early and late flowering of Brassica oleracea and application thereof, wherein the nucleotide sequence of the molecular marker exists in the non-coding region of chromosome 4 of the genome of early flowering Brassica oleracea, and does not exist in the non-coding region of chromosome 4 of the genome of late flowering Brassica oleracea; based on the molecular marker, a primer pair Bf5 is further designed, DNA of the Brassica oleracea is amplified by using the primer pair, and early flowering varieties or materials of the Brassica oleracea can be screened according to the presence or absence of the target band. The molecular marker and the primer pair provided by the application can be used for rapidly detecting early flowering varieties and materials of the Brassica oleracea, time and resources are greatly saved, selection efficiency is greatly improved, and thus the breeding process is accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a local chicken body type character, the SNP molecular marker is located on a chicken chromosome 4, the number of the SNP molecular marker is Chr475876885, and the genome version is GRCg6a; the nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, the SNP molecular marker is located at the 401bp of the nucleotide sequence of the SEQ ID NO.1 and has C / T polymorphism, and the genotype of the site of the polymorphism is that TT corresponds to a large-size individual, and the genotype of CC corresponds to a small-size individual. According to the SNP molecular marker related to the body type character of the local chicken, individuals are directly bred on the genome level, phenotypic information is not depended on, the selection efficiency can be remarkably improved for characters which are low in heritability and difficult to measure, and the breeding process can be accelerated.

The application provides a SNP marker on a pig chromosome 4 affecting pig body size traits. The sequence of the nucleic acid of the SNP marker is shown as SEQ ID No. 1, and the site of the SNP marker is located at the 301th position from the 5' end on the nucleic acid shown as SEQ ID No. 1, corresponding to the 75643517th position from the 5' end on the chromosome 4 of the international pig genome version 11.1, being T or C.

This invention discloses a SNP molecular marker located on pig chromosome 4 that is associated with waist circumference and its application. The SNP molecular marker is located at position 134 from the 5' end of the nucleotide sequence shown in SEQ ID NO:1, corresponding to position 22721111 bp on chromosome 4 in International Pig Reference Genome Version 11.1, where a C>T mutation exists. The SNP molecular marker provided by this invention is significantly correlated with the waist circumference trait in pigs. By identifying the genotype of this SNP molecular marker, the waist circumference trait in pigs can be identified. Furthermore, by selecting pigs with the CC genotype of the SNP molecular marker, the pig breeding process can be accelerated, achieving genetic improvement of pigs.

The invention relates to the field of molecular biology and genetic breeding, and provides application of an SNP (Single Nucleotide Polymorphism) marker related to a horse body height character. The SNP marker is located at the 16200793bp position of a chromosome 4 of a horse, and is Agt; and C base mutation (rs1138581426) is carried out. Experiments show that the developed SNP marker has significant influence on the horse body height character (Plt; 0.05), and the allele A has more excellent body height character, so that the SNP molecular marker in the IGFBP1 gene can be applied to horse body height character molecular marker breeding. The molecular marker provided by the invention is not limited by the age, sex and the like of the horse, can be used for breeding an excellent and high horse variety, and even can be accurately screened when the horse is newly born, so that the breeding process of the excellent and high horse variety is greatly accelerated.

The application provides a SNP molecular marker related to a body weight trait of a local chicken and an application thereof, and belongs to the technical field of molecular biology and breeding. The SNP molecular marker is located at the 77325222th nucleotide of chromosome 4 in the international chicken reference genome GRCg6a Primary Assembly version, has C / T polymorphism, and the 18-week average body weight of an individual with a CT or TT genotype is significantly higher than that of an individual with a CC genotype. The application further provides a primer combination for detecting the above SNP marker and an application thereof. The SNP molecular marker and the primer combination can be used to establish a high-efficiency and accurate molecular marker-assisted breeding technology for the body weight trait of the local chicken, so that the body weight trait of the local chicken is effectively improved, and the high-quality development of the local chicken industry is promoted.

The invention discloses a molecular marker related to corn row grain number and application thereof, the molecular marker is based on a corn ZmSBP20 gene promoter region, the ZmSBP20 is located on a corn chromosome 4, the physical position of the ZmSBP20 on a corn B73 reference genome Zm-B73-REFERENCE-NAM-5. 0 is Chr4: 246387302-246390553, the corresponding physical position of the molecular marker is Chr4: 246391202-246391344, and the ZmSBP20 is located on the corn ZmSBP20 gene promoter region. The base sequence of the InDel + genotype at the position is as shown in SEQ ID No.1, and the InDel-genotype at the position is deleted by 142 bp. The molecular marker has the characteristics of stable amplification, high detection efficiency and the like, can accurately, efficiently and stably distinguish the InDel + genotype from the InDel-genotype, can be widely applied to molecular marker-assisted selection of favorable variation sites of row grain number, and provides important technical support and theoretical basis for genetic improvement of corn yield.

The invention belongs to agricultural biotechnology engineering, and particularly discloses a rice seedling stage cold tolerance locus qCTS4 and a molecular marker and application thereof. The molecular marker is a locus qCTS4 which is positioned at 32446985bp of a chromosome 4 and is remarkably related to the cold resistance in the seedling stage, and the cold resistance of the germplasm carrying A nucleotide allelic variation at the locus in the seedling stage is extremely remarkably higher than that of the germplasm carrying G nucleotide allelic variation. The method for identifying the cold resistance of the rice in the seedling stage is accurate and reliable through verification.

The invention relates to the technical field of plant biology, and particularly provides an SNP site for detecting the banding character of peas and application of the SNP site, the SNP site is located at the 16601390th nucleotide site of a chromosome 4 of a pea genome (CAASPsatZW61.0), and the basic group of the SNP site is G or T. Based on the SNP site, the invention develops a KASP molecular marker, and a primer group of the KASP molecular marker comprises sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 3. KASP genetic typing is carried out through the primer group, the character of peas to be detected can be judged, and when the genotype is GG, the character is banded; when the genotype is TG or TT, the gene shows a normal phenotype. Through verification, the KASP molecular marker shows a group typing effect with high consistency and clear boundary in group typing. The KASP marker provided by the invention can realize early, rapid and low-cost detection of the banding character of peas, and is suitable for molecular marker-assisted breeding of peas.

The application belongs to the technical field of molecular biology and molecular marker, and particularly relates to a SNP molecular marker related to a pig girth trait on a chromosome 4 of a pig and application thereof. The SNP molecular marker related to the pig girth trait on the chromosome 4, wherein a SNP site corresponds to a T>G mutation at position 122124975 on a chromosome 4 of an international pig reference genome version 11.1, and the polymorphism of the base at the site influences the pig girth trait. The application can increase the frequency of the dominant allele generation by generation by selecting the dominant allele of the SNP, improve the pig girth trait, select the excellent germplasm of the above traits, accelerate the progress of pig genetic improvement, and thus effectively improve the economic benefits of pig breeding.

The present invention provides an application of an SNP genetic marker affecting the shank circumference of chickens in chicken genetic breeding, belonging to the fields of chicken genetic breeding and biotechnology. The SNP genetic marker affecting the shank circumference of chickens includes SC_tag4, and the SC_tag4 corresponds to the physical position of the 75,909,527th site on chromosome 4 of the chicken reference genome bGalGal1.mat.broiler.GRCg7b version sequence information published in NCBI, belonging to the 8th intron sequence of the gene LDB2, where the base is T or C. This SNP genetic marker helps to genetically improve the shank circumference. Applying it to chicken genetic breeding is beneficial to improving the shank circumference of laying hens and reducing the incidence frequency of osteoporosis, thereby obtaining high-quality laying hen breeds.

The invention relates to a molecular marker Marker 2937 related to the fruit hardness of sweet cherries and application of the molecular marker Marker 2937. The amplification result of the three KSASP primers designed by the invention is that the 16080735bp position of the chromosome 4 of the sweet cherry genome is an SNP site, the genotype of the SNP site is defined as T / T in a hard fruit material, and the genotype of the SNP site is defined as A / A in a soft fruit material. The amplification effect can obviously and effectively distinguish the genotype of the SNP site in the sweet cherry fruit, the nucleotide mutation site can be rapidly and accurately detected, and an accurate, rapid and effective method is provided for breeding of a hard sweet cherry variety. The development of the KASP marker can improve the identification and screening of fruit hardness genotypes of filial generations of different sweet cherry varieties, a phenotype identification method is not adopted, the breeding efficiency can be obviously improved, and the breeding cost is saved.

This invention discloses a SNP molecular marker associated with skin color traits in Ma Huang chickens and its application, belonging to the field of genetic breeding technology. This invention identifies an SNP locus at position 49,442,841 in the intron region of the SCARB2 gene on chicken chromosome 4. This locus contains a T>G mutation, with genotypes including TT, TG, and GG. This locus is significantly correlated with the yellowness of the skin on the chest, back, and legs. Individuals with the TT genotype show significantly higher yellowness values ​​on the chest, back, and legs than individuals with the TG genotype. This indicates that this SNP locus can accurately identify skin color traits in chickens and can serve as an important genetic marker for early screening of skin color traits during chicken breeding. Early selection of chickens based on the genotype at this locus can save production costs and accelerate genetic progress.

The invention provides an SNP molecular marker for detecting soybean quality genes, and belongs to the technical field of soybean quality detection. The sequence of the SNP molecular marker is shown as SEQ ID NO.1, the SNP molecular marker is located on the No.4 chromosome of the soybean, a G / A polymorphic site exists at the 80bp in the SEQ ID NO.1, and an A / T polymorphic site exists at the 2648bp in the SEQ ID NO.1; the 80bp site and the 2648bp site are respectively located at the 46951621st site and the 46954189th site of the coding gene on the soybean chromosome 4, the genotype of the 46951621st site comprises the genotypes of GG, AA and GA, and the genotype of the 46954189th site comprises the genotypes of AA, TT and AT. The genotype GG-AA is a haplotype 1 (Hap1), and the genotype AA-TT is a haplotype 2 (Hap2).

The invention relates to a molecular marker primer related to duck black matter characters and application of the molecular marker primer, and belongs to the technical field of biology. The KIT gene SNP molecular marker is located at the 51648174 basic group of chromosome 4 of a duck reference genome GCF047663525.1 version, the basic group is mutated into T or C, and the genotypes are T / T, T / C and C / C. The ducks of the C / C and T / C genotype individuals show a ubiquitous phenotype, and the ducks of the T / T genotype individuals show a non-ubiquitous phenotype. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing and genetic typing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used for efficiently and accurately identifying the duck black matter character genotype, the accuracy and efficiency of duck black matter character identification are improved, and the molecular marker has important value for protection and utilization of excellent germplasm resources of black-bone ducks.

The present application relates to pig breed molecular identification, and discloses a SNP molecular marker combination for Wannan flower pig identification, the combination is composed of the following 11 SNP sites, the physical position and allele information of each SNP site is determined based on pig reference genome Sscrofa11.1: rs342792776, located at 103395244bp of chromosome 4, the allele thereof is A / G; rs343665183, located at 104083270bp of chromosome 4, the allele thereof is G / A; novel_WNS1, located at 5240118bp of chromosome 7, the allele thereof is G / A.The present application is based on whole genome resequencing and population genetics analysis, 11 Wannan flower pig specific SNP sites are screened out, and unified REF / ALT unification, 0 / 1 / 2 numerical coding, standardization processing and threshold determination rules are combined, so that the detection process is clear, the repeatability is strong, and the standardized implementation between different detection platforms and laboratories is facilitated.

The present invention provides a molecular marker located on chromosome 4 and related to drought resistance of alfalfa and its application, belonging to the field of biotechnology. The nucleotide sequence of the inserted or deleted fragment is shown in the sequence table SEQ ID NO.3. The primer pair used to amplify the molecular marker has a nucleotide sequence shown in SEQ ID NO.1-2. The molecular marker and the primer pair described in the present invention can identify or assist in identifying the drought resistance trait of alfalfa. At the same time, the present invention also provides a method for rapidly identifying the drought resistance and drought-resistant genotype of alfalfa using the molecular marker. The method is simple and rapid, the identification results are accurate, and it has good prospects for promotion and application.

A technique for selecting sugar beet plants that are resistant to chlorosis virus disease is provided. [Solution] A method for determining whether or not a sugar beet plant has resistance to chlorosis virus disease includes a step of determining whether or not the sugar beet plant has resistance by identifying at least one of the gene loci involved in resistance to chlorosis virus disease located between 11.0 Mbp and 30.0 Mbp on chromosome 4 and between 8.0 Mbp and 38.0 Mbp on chromosome 7 in the sugar beet plant.

The application discloses a chicken back hair follicle density character detection method and a PCR detection primer pair and detection kit, relates to the field of poultry genetic breeding and biotechnology, and comprises the following steps: (1) detecting the genotype of a to-be-detected chicken SNP-FAT1; the SNP-FAT1 is located at the 61238343th site of a chicken chromosome 4, the base of the site is A or C, and the corresponding genotype comprises three types of CC, CA and AA; (2) judging the back hair follicle character of the to-be-detected chicken according to the detected genotype. The application finds the correlation between a specific gene SNP-FAT1 of a chicken and the back hair follicle density character of the chicken, and according to the detected genotype, the back hair follicle density character of the chicken can be selected in vivo, the accuracy of selection can be obviously improved compared with traditional sibling selection, the hair follicle density of a breed can be stably improved by continuously applying the method, and the breeding benefit of the breed is increased.

The invention provides application of an SNP (Single Nucleotide Polymorphism) genetic marker influencing the laying weight of chickens in genetic breeding of laying hens, and belongs to the technical field of animal genetic breeding and biology. The SNP genetic marker influencing the laying weight of the chickens comprises bw1egg1 and / or bw1egg2; the Ensemb l number of the bw1egg1 is rs318020581, corresponds to the 76311052nd site of a positive-sense strand of a chromosome 4 of a chicken reference genome bGa < 1 > Ga < 1. Mat.broi ler.GRCg7b sequence published in NCBI (National Center of Biotechnology Information) and belongs to the third intron of a gene C1QTNF7, and a basic group in the site is T or G; the Ensemb l of the bw1egg2 is numbered as rs313708699, corresponds to the 74731889 site of a positive-sense strand of a chromosome 4 of a chicken reference genome bGa < 1 > Ga < 1. Mat.broi ler.GRCg7b sequence disclosed in NCBI (National Center of Biotechnology Information), and belongs to the eighth intron of a T2 gene of a gene SLI, and a basic group is T or C at the position. The bw1egg1 and the bw1egg2 are both beneficial to heredity improvement of the first laying weight, the bw1egg1 and the bw1egg2 are applied to genetic breeding of chickens, improvement of the first laying weight of laying hens is facilitated, and a laying hen variety with excellent first laying weight uniformity is obtained.

The application discloses a method for identifying duck palm weight and a related molecular marker application, relates to the technical field of molecular marker assisted selection, and a primer pair is used for amplifying a DNA fragment containing a polymorphic site of a duck reference genome IASCAAS_PekinDuck_T2T chromosome 4 from the 5' end 63823029 base position; the duck reference genome IASCAAS_PekinDuck_T2T is a duck reference genome sequence in a GenBank database; the duck is a Zhongxinbaihu meat duck, and the primer pair consists of a DNA molecule shown in SEQ ID No. 2 and a DNA molecule shown in SEQ ID No. 3. The method for identifying the duck palm weight and the related molecular marker application can determine the genotype of the duck by detecting the genomic DNA of the duck, realize early in-vivo screening of the duck palm weight trait, do not need to wait for post-slaughter determination of a phenotype, significantly shorten a breeding cycle, and reduce breeding cost.

The invention discloses an SNP molecular marker related to carcass skin color traits of spotted-brown chickens and application of the SNP molecular marker, and belongs to the technical field of genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site is found at the 49442841st site of an intron region of an SCARB2 gene of a chicken chromosome 4, and Tgt exists at the site; the SNP locus is a single nucleotide polymorphism (SNP) locus, has G mutation, genotypes including TT, TG and GG genotypes and is remarkably related to skin yellowness values of breasts, backs and legs, and the skin yellowness values of the breasts, backs and legs of individuals with TT genotypes are remarkably higher than those of individuals with TG genotypes, so that the SNP locus can accurately identify skin color traits of chicken carcasses. The genetic marker can be used as an important genetic marker for early screening of carcass skin color traits in a chicken breeding process. According to the genotype of the site, the chicken carcass skin color traits can be subjected to early selection, the production cost is saved, and the genetic progress is accelerated.

This invention discloses a SNP marker and detection primer pair associated with the adventitious root primordium trait of *Senecio scandens* with stem nodules, and its application, belonging to the field of molecular biology technology. Based on SNP marker technology, this invention develops an SNP marker and detection primer pair linked to the adventitious root primordium trait of *Senecio scandens* with stem nodules. This invention involves phenotypic identification of mutants induced by EMS mutagenesis in *Senecio scandens* with stem nodules, followed by isolation, purification, population construction, and genetic analysis combined with Mutmap. + The SNP marker associated with adventitious root primordia is located at 10,433,968 bp on chromosome 4 of *Senecio scandens*, involving a C / T mutation. When the genotype is T, *Senecio scandens* lacks adventitious root primordia. This invention's SNP molecular marker can screen F2 populations at the early cotyledon stage, identifying mutants and wild types without phenotypic differences, thus improving screening efficiency, shortening the breeding cycle, and demonstrating value in marker-assisted breeding.

This invention discloses a molecular marker associated with the growth and development traits of yellow-feathered broiler chickens and its application, belonging to the field of molecular breeding technology. The SNP molecular markers correspond to the sequence information of chromosome 4 in the Gallus_gallus-6.0 version of the chicken reference genome published in NCBI, specifically at 75971034 bp (genotypes GG, GC, CC) and 75971177 bp (genotypes GG, GA, AA). The 75971034 bp genotype is a favorable genotype with the GG genotype at the locus, carrying significantly higher mid-metabolic weight, 87-day weight, and 113-day weight compared to individuals carrying the CC genotype. The 75971177 bp genotype is also a favorable genotype with the GG genotype at the locus, carrying significantly higher mid-metabolic weight compared to individuals carrying the AA genotype. Using these molecular markers for molecular-assisted selection allows for early screening of mid-metabolic weight, 87-day weight, and 113-day weight, effectively reducing production costs, promoting genetic improvement, and demonstrating high economic benefits and application value.