The invention relates to a negative liquid crystal composition with a terminal alkene structure and application of the negative liquid crystal composition. The liquid crystal composition contains liquid crystal compounds shown as general formulas I, II, III, IV and V which are described in the specification. The negative liquid crystal composition has negative dielectric anisotropy, high gradient,especially low viscosity and short response time, has good stability and low-temperature intersolubility, strong UV resistance and high VHR and resistivity, and can be applied to display modes of VA,MVA, PVA, FFS, PSVA, IPS, TFT and the like.