A strain of Bacillus thuringiensis and its application

A technology of Bacillus chrysogenum and strains, applied in the fields of application, bacteria, biocide, etc., can solve the problems of less research reports on edible fungus fungus, safety cannot be guaranteed, residues, etc., and achieve good application and promotion prospects and good economics and ecological benefits, good insecticidal activity

Active Publication Date: 2022-07-29
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus thuringiensis has the advantages of strong specificity, harmlessness to humans and animals, and environmental friendliness. Especially for edible fungi, which have a short time from fruiting body formation to harvesting, the safety requirements are more stringent. The use of chemical pesticides on edible fungi is likely to cause phytotoxicity and residues, and the safety cannot be guaranteed, but not all Bt strains can be used to control the fungus in the cultivation of edible fungi
[0004] At present, there have been many reports on the research on the use of Bacillus thuringiensis to prevent and control Diptera pests. For example, the control effect of Bt on chive eye fungus, culex, Aedes, melon fly, etc. is more than 70%. There are few research reports on Mycophthalmus

Method used

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  • A strain of Bacillus thuringiensis and its application
  • A strain of Bacillus thuringiensis and its application
  • A strain of Bacillus thuringiensis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Strain isolation and purification

[0024] A 10 g soil sample was taken from 2 cm below the topsoil of Zijin Mountain, Nanjing City, Jiangsu Province, and Bacillus thuringiensis (Bt) was isolated by the sodium acetate-antibiotic method. The specific separation method is as follows: Weigh 1g soil sample, pour it into glass beads and 50mL separation medium (yeast powder 5g / L, peptone 10g / L, sodium chloride 10g / L, sodium acetate 34g / L, pH 7.0) 0.1 mL of penicillin sodium salt and gentamicin sulfate with a concentration of 0.2 g / mL were added to the culture medium, respectively, and cultured on a shaking table (190 r / min, 30 °C) for 4 h. After the cultivation, 10 mL of soil suspension was taken and placed in a water bath at 80°C for 20 min, the soil suspension was diluted 10 times and 100 times respectively, and 0.1 mL of each was evenly spread on the sporulation medium plate (sporomorphic medium: beef extract). 5g / L, peptone 10g / L, agar 20g / L, pH 7.0), the plat...

Embodiment 2

[0025] Example 2: Strain identification

[0026] After strain JW-1 was streaked and cultured on solid medium for 24 hours, it was observed that the colony was milky white, flat, and the surface was dry and dull; it could grow on various mediums such as LB and PDA, and it was aerobic bacteria; through cryo-electron microscopy Observed( figure 2 ) JW-1 strain vegetative body is rod-shaped, spores are round, and paraspore crystals are irregular. The results of Gram staining and API 50CH standardization system test are shown in Table 1. The JW-1 strain can utilize mannitol, D-ribose, D-glucose, D-fructose, D-mannitol, N-acetylglucosamine, Esculin-citric acid, ARBULIN, D-cellobiose, D-maltose, D-trehalose, starch, glycogen and other 13 substances, the rest cannot be used. This result is consistent with Bacillus thuringiensis in the comparison library.

[0027] Table 1 Physiological and biochemical characteristics of JW-1 strain

[0028]

[0029] Note: "+" indicates positive...

Embodiment 3

[0031] Example 3: Identification of strain insecticidal protein genotype

[0032] Using the genomic DNA of the JW-1 strain as a template, a variety of insecticidal protein universal primers were used for PCR amplification, and it was determined that the JW-1 strain contained 6 insecticidal proteins including cry4a, cry4b, cry / 10, cry11, cyt1 and cyt2. genotype.

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Abstract

The invention relates to a strain of Bacillus thuringiensis JW-1, which is preserved in the General Microorganism Center of the China Microorganism Culture Collection and Management Committee, and the preservation number is CGMCC NO.17571. The 16S rDNA nucleotide sequence of the Bacillus thuringiensis JW-1 is shown in SEQ ID NO.1. The Bacillus thuringiensis JW-1 has good insecticidal activity against the edible fungus Elephantidae, and the present invention utilizes the advantages of good insecticidal effect and safe mycelial growth to produce safe and efficient edible fungi used in edible fungi. The insecticide can be used to specifically kill the edible fungus eye fungus, replaces the use of some chemical pesticides, reduces environmental pollution, has good economic and ecological benefits, and has a good application and promotion prospect.

Description

technical field [0001] The invention relates to a strain of Bacillus thuringiensis JW-1 and its application in the prevention and control of edible fungi and insect pests, belonging to the field of biotechnology. Background technique [0002] Sciaridae belongs to Arthropoda, Isecta, Diptera, and mainly harms Agaricus bisporus, Oyster mushroom, Xiuzhen mushroom, Tea tree mushroom, Auricularia, Morel, Black There are many varieties of edible and medicinal fungi such as skin chicken fir, straw mushroom, ganoderma lucidum, etc., and they occur in all production areas of the country. There are Bradysia difformis, Lycoriella ingenua and so on. After hatching, the mosquito larvae directly feed on the mycelium and fruiting bodies, and excrete feces to breed bacteria. Adult worms are important vectors for the spread of pathogenic bacteria, nematodes, and mites. At present, the prevention and control methods for ophthalmia ophthalmosus in edible fungus cultivation mainly include ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/23A01P7/04C12R1/07
CPCC12N1/20A01N63/00C12R2001/075C12N1/205
Inventor 马林曲绍轩林金盛侯立娟李辉平蒋宁王帆帆骆昕
Owner JIANGSU ACAD OF AGRI SCI
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