The invention discloses a novel making method of a skinned specimen of fish. The method comprises steps as follows: (1), the fish is anesthetized and killed, and mucus is cleaned; (2), dimensions of the fish are measured and photographed; (3), the posture of a fish body is set, and a skeleton diagram is drawn; (4), cutting as well as peeling and carving of prosthesis is performed; (5), a proper notch is selected and skin is peeled off; (6), the fish skin is subjected to degreasing and preservative treatment; (7), the prosthesis is smeared with dextrin, skin suture is performed, and natural drying is performed after shaping; (8), repair and inlaying of ocular prosthesis are performed; (9), water-based acrylic acid modified polyurethane dispersion is adopted for coloring, and wood lacquer issprayed after coloring. The method has the advantages as follows: preparation is simple, and cost is low; fish postures can be adjusted according to requirements in the preparation process; a morphological structure is measured scientifically and systematically; the prepared fish skinned specimen is environmentally friendly, non-toxic, resistant to high temperature, acid and alkali, anti-aging and easy to store; the structure is complete and attractive, detailed parts are vivid and lifelike, and the specimen is widely applied to teaching, scientific research, collection and ornamentation.