The invention discloses a vehicle lane separation system, which comprises a main road, an auxiliary road and several partition assemblies, the partition assembly is used to separate the main road and the auxiliary road; the main road is used for driving cars and trucks, and the auxiliary road is only used for driving cars ; The separation assembly includes several isolation rods and lifting mechanisms, and a control assembly is provided on the main road. The control assembly includes a gravity sensor, a speedometer, a voice reminder and a controller. The controller is provided with a threshold. When the weight of the vehicle is less than the threshold At this time, the controller triggers the lifting mechanism to work, driving several isolation rods to move downward, and at the same time, the voice reminder reminds the driver of the driving vehicle to divert voice, and the car will drive from the main road to the auxiliary road. Since there are only cars driving on the auxiliary road, so The car can run on the auxiliary road at a higher speed; that is, the driving safety is guaranteed, and traffic jams and car accidents are not easy to occur; at the same time, the driving time will not increase, and the destination can be reached on time.