The invention discloses a vehicle lane separation system. The vehicle lane separation system involves a main lane, an auxiliary lane and a plurality of separation components, wherein the separation components are used for separating the main lane from the auxiliary lane, the main lane is for automobile and truck driving, and the auxiliary lane is only for the automobile driving; each separation component comprises a plurality of isolation rods and a lifting mechanism; a control component is arranged on the main lane, the control component comprises a gravity sensor, a speed measuring instrument, a voice reminder and a controller; the controller is provided with a threshold value, when the weight of a running automobile is smaller than the threshold value, the controller triggers the lifting mechanisms to work to drive the plurality of isolation rods to move downwards, meanwhile, a voice lane-changing reminder is carried out on a driver of the running automobile by the voice reminder, the automobile can run from the main lane to the auxiliary lane, due to the fact that only the automobile runs on the auxiliary lane, the automobile can run on the auxiliary lane at a high speed; the driving safety is ensured, and traffic jams and traffic accidents are not easy to happen; and meanwhile, the driving time is not prolonged, so that the driver can reach a destination on time.