The invention relates to a fluorescent probe for fluorescent quantitative PCR reactions. The fluorescent probe is characterized in that the probe comprises an oligonucleotide sequence, the 5' terminus is a specific oligonucleotide sequence with an amplified target sequence and modifies the fluorescence groups; the 3' terminus is a nucleotide sequence, forms a reversely-complimentary palindrome structure with the 5' terminus, and modifies the fluorescence quenching groups. Under certain conditions, the probe can form an intramolecular secondary composition, and the fluorescent groups get close to the quenching groups so as to quench the fluorescence signals released by the fluorescence groups. Compared to the common fluorescent probe, the provided fluorescent probe has the advantages of high specificity, high sensitivity, and low fluorescent quenching efficiency, thus the signal to noise ratio (SNR) of the reactions is higher, and the provided fluorescent probe can be applied to realtime fluorescence quantitative PCR detection reactions and all end point fluorescence detection reactions.