The invention discloses a charging method of an electric bicycle charging station capable of reserving charging. The electric bicycle charging station includes a cloud server, a user mobile phone, a charging card, charging piles dispersed at every place of a city and electric bicycles to be charged. The charging method includes steps of step 1, reserving a charging pile; step 2, connecting the charging pile; step 3, identifying charging power of the charging pile; step 4, carrying out prepayment by the user; step 5, monitoring the charging of the charging pile in real time; step 6, calculating the charging cost; step 7, returning rest amount; step 8, informing user of completing charging. The charging method can realize the charging reservation, and provide convenience for user payment through card reading, Wechat, Alipay and other payment methods; meanwhile, the method can accurately calculate the power consumption of the user, reasonably charge, and correct the settlement unit from 'Yuan' to 'cent'; thus the reasonability of the electric charge calculation is greatly improved.