Composition containing bifidobacteria for adjusting intestinal flora and enhancing immunity

A technology that regulates intestinal flora and enhances immunity. It is applied in the direction of drug combination, microbial-based methods, and active ingredients of hydroxyl compounds. Achieve the effect of inhibiting the growth of spoilage bacteria, reducing the culture volume, and inhibiting the change of physical and chemical properties

Inactive Publication Date: 2011-04-20
菲伯纳生物医药有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN 1056877C discloses a bifidobacterium microcapsule and its manufacturing method, CN 1063051C discloses a bifidobacterium compound living bacteria preparation, and CN 1149092C discloses an acid-resistant enteric-coated microcapsule of active bifidobacterium strain , the three patents all use microencapsulation technology, which enhances the ability of bifidobacteria to resist the external environment, and can resist the erosion of gastric acid and bile salts. It can still effectively protect its activity when stored in a liquid state or at room temperature for a long time. However, Generally, it takes more than six hours for bifidobacteria embedded in microcapsules to dissolve and release after reaching the intestinal tract, and the effect is limited
Patent CN 1116410C discloses a high-viability live bacteria preparation and its preparation method. In this patent, Bifidobacteria are subjected to liquid deep-layer high-density culture, and the number of viable bacteria in the fermentation liquid is 5×10 8 cfu/mL, the bacteria adopts four protection measures of fre

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  • Composition containing bifidobacteria for adjusting intestinal flora and enhancing immunity
  • Composition containing bifidobacteria for adjusting intestinal flora and enhancing immunity
  • Composition containing bifidobacteria for adjusting intestinal flora and enhancing immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation of Bifidobacterium powder

[0042] 1. High-density cultivation

[0043] (1) Bacteria activation: Under aseptic conditions, pick the bacteria in the ampoule tube and inoculate them on liquid culture medium and agar plates, culture them anaerobicly at 37°C for 48 hours, and activate continuously for 3 generations to obtain activated bacteria. .

[0044] Among them, the preparation method of the bifidobacteria medium used in the present invention is as follows: tryptone 5.0g, yeast extract 10.0g, soy peptone 5.0g, glucose 10.0g, L-cysteine ​​hydrochloride 0.5g, vomiting Warm 801.0mL, salt solution 40mL, add water to make the volume to 1000mL, adjust pH 6.8~7.0, sterilize at 121℃ for 20min. The composition of the salt solution is: calcium chloride 0.2g, potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.48g, sodium bicarbonate 10.0g, dipotassium hydrogen phosphate 1.0g, sodium chloride 2.0g, and the volume is made up to 1000 mL with water.

[0045] (2...

Embodiment 2

[0060] Example 2: Composition formula

[0061] Component

[0062] The components of the composition are mixed according to the above-mentioned dosage to make a capsule, 0.25g / capsule, 4 capsules each time, 2 times a day; to make an granule, 1g / packet, 1 bag each time, 2 times a day.

Embodiment 3

[0063] Example 3: Function test of regulating intestinal flora in normal mice

[0064] Animal grouping and administration: 40 BALB / C inbred mice, male, 6-8 weeks old, 18-22g / mouse, randomly divided into blank control group and composition low, medium and high dose groups, each with 10 only. The low, medium and high dose groups were given the composition 0.17, 0.33, and 1.0 g / kg (equivalent to 5, 10, and 30 times the recommended human dose) by intragastric administration. The blank group was given normal saline once a day for 14 days, and the indicators of body weight, Bifidobacterium, Lactobacillus, Enterococcus, Enterobacter, and Clostridium perfringens were checked regularly.

[0065] Detection method: Before administering the composition, aseptically take 0.1g of mouse feces and dilute it to 10 times serially. -8 , Select the appropriate dilution to inoculate each medium separately. After culturing, the colonies are identified and counted by colony morphology, Gram stain micro...

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PUM

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Abstract

A composition containing bifidobacteria for adjusting intestinal flora and enhancing immunity is characterized by comprising, by weight, 0.01-10% of bifidobacteria, 10-50% of oligosaccharide, 0.01-10% of vitamin, and 0.01-10% of metals, with the balance being auxiliary materials. The composition containing bifidobacteria provided by the invention has functions of adjusting intestinal flora and enhancing immunity. The composition can be made into capsules, soluble granules, granules, tablets, etc., and applied in food, health product and pharmaceutical fields.

Description

Technical field [0001] The invention relates to a bifidobacterium product, in particular to a bifidobacterium-containing composition for regulating intestinal flora and enhancing immunity, belonging to the technical field of bifidobacterium product preparation. Background technique [0002] The intestinal microecosystem is the largest and most complex microecosystem of the human body, and it is also the most important and active part of the human microecosystem. Intestinal flora is an important part of the intestinal defense barrier. A balanced intestinal flora is very important to human health. However, due to the aging of the body, environmental pollution, dietary changes, and the abuse of antibiotics, the human intestinal microecosystem is often destroyed, causing imbalance of the intestinal flora, seriously affecting the body’s immune system, resulting in weakened immune function and leading to bacteria Group translocations induce endogenous infections, cause body diseases, ...

Claims

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Application Information

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IPC IPC(8): A23L1/302A23L1/304A23L1/303A61K35/74A61P1/00A61P37/04C12N11/04A61K31/702A61K31/07A61K31/51A61K31/714A61K31/525A61K31/4415A61K31/375A61K31/59A61K31/355A61K33/30A61K33/26A61K33/06C12R1/01A23L33/16A61K35/745
Inventor 曾星
Owner 菲伯纳生物医药有限责任公司
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