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Grape branch extract and preparation method and application thereof

An extract and branch technology, applied in the field of grape branch extract and its preparation, can solve the problems of unknown composition and content of the extract, skin damage caused by ethanol, and incomplete extraction, so as to inhibit the formation of pigmentation and nourish the skin for a long time , skin-friendly effect

Inactive Publication Date: 2016-04-06
SHANGHAI SHENYIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Grape is one of the plants with the most abundant content of resveratrol or its dimer (viniferine), and PCT patent application WO01 / 03713 discloses a method for extracting resveratrol and / or its dimer (viniferine), and Point out its anti-oxidation properties, however, resveratrol flavonoids are difficult to dissolve in water, and need ethanol or glycerin as a solvent to extract, this extraction method has certain damage to the skin
[0005] Japanese patent JP2014198042A discloses a method for extracting and preserving the liquid in grape vines. It uses 50% ethanol to cook and extract, which breaks the limitations of the seasons. However, this method uses high-temperature cooking for extraction, and the extraction is not thorough. The obtained extract The ingredients and content are unknown, and the large amount of ethanol used in the extract will cause certain damage to the skin

Method used

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  • Grape branch extract and preparation method and application thereof
  • Grape branch extract and preparation method and application thereof
  • Grape branch extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Utilize grape branch to prepare grape branch extract, comprise the following steps:

[0034] 1) Cut 10 grams of grape branches into sections, remove the epidermis, wash them, and disinfect them with 75% alcohol for 5-10 minutes;

[0035] 2) Freeze-dry the sterilized grape branches with liquid nitrogen, grind them into powder, add 40 ml of water, heat to 40-60°C, and ultrasonically extract for 2 hours;

[0036] 3) Centrifuge after the leaching, filter, collect the filtrate, and then filter with a filter membrane with a pore size of 0.22 μm to obtain 25 milliliters of grape branch extract aqueous solution, hereinafter referred to as the extraction stock solution, wherein the mass fraction of grape branch extract is 5 to 10 %.

[0037] 1 milliliter of the extraction stock solution of the present embodiment contains proanthocyanidins (about 71.4mg / L) of vine source, lysine (about 64.2mg / L), proline (about 7.3mg / L), glycine (about 10.3mg / L) / L), iron ion (4.8mg / L), the sto...

Embodiment 2

[0039] Hydroxyproline is an important amino acid with a stable content (accounting for 13.4%) in collagen. It is very small in elastin and does not exist in other proteins. Therefore, the determination of its content is a reliable indicator for detecting collagen synthesis ability. Method, the detection of hydroxyproline comprises the steps:

[0040] 1. Preparation of Human Skin Cells

[0041] Take normal human dermal fibroblasts as primary cells, and place them in a 75cm 2 In a culture flask (2ml of culture medium containing growth factors), 37°C, CO 2 The cells were cultured at a concentration of 5% to a concentration of 80%. Passage, passage cells 175cm 2 Continue to culture the cells in the square flask to 80% concentration.

[0042] 2. Preparation of samples to be tested

[0043] Culture the human dermal fibroblasts obtained in the previous step with DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Cultured for 24 hours, when the cells were close to conf...

Embodiment 3

[0058] Carry out hyaluronic acid content determination to the culture supernatant of A sample, B sample, C sample, D sample and control group sample of step 2 of embodiment 2, comprise the following steps:

[0059] 1. According to the manual of Human Hyaluronic Acid ELISA Kit (Shanghai Enzyme Biotechnology Co., Ltd.), draw a hyaluronic acid standard curve, see image 3 :

[0060] Prepare 6 hyaluronic acid standard solutions, the concentrations are: 0μg / mL, 10μg / mL, 20μg / mL, 40μg / mL, 80μg / mL, 160μg / mL, and use a microplate reader to test its OD value at 450nm, test See Table 3 for the results;

[0061] table 3

[0062] Hyaluronic acid standard (μg / mL)

[0063] According to the test results of hyaluronic acid standard products, the absorbance is taken as the vertical axis, and the hyaluronic acid concentration is taken as the horizontal axis to draw the standard curve of hyaluronic acid, see image 3 ;

[0064] OD=0.0136×c+0.297, R 2 =0.958, p=0.00068, wherein, c ...

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Abstract

The invention provides a grape branch extract and a preparation method and application thereof. Grape branches are extracted by adopting a water extraction mode, multiple active substances from the xylem and the phloem of a grapevine are obtained, and a grape branch extract containing grapevine source plant polyphenol substances, polysaccharide, amino acid and iron ions is obtained. The grape branch extract has the significant effects on promoting proliferation of multiple cells in the dermis and generating beneficial substances such as collagen, can promote generation of water locking substances such as ceramide and hyaluronic acid of the skin, also can reduce pigmentation, inhibit color sport generation and enable the skin to be fair and bright, has the high safety, the high oxidation resistance, the significant acceleration on collagen secretion of a cytoplasmic matrix and the obvious skin moisturizing effect and can be applied to development of anti-aging and skin whitening or / and moisturizing cosmetics and health-care products.

Description

technical field [0001] The invention belongs to the field of plant component extraction, and in particular relates to a grape branch extract and its preparation method and application. Background technique [0002] In today's society, with the rapid development of production and economy, air pollution is also becoming more and more serious, and the destruction of the ozone layer is deepening. Ultraviolet radiation, electromagnetic radiation, automobile exhaust, industrial waste gas and air dust all have a serious impact on human health. PM2.5 has become a hot topic. It contains heavy metals such as nitrogen compounds, sulfide compounds, and lead. These toxic substances can enter the pores of the skin, causing clogged pores and blackheads, and ultimately lead to dull skin. Poor skin metabolism eventually accelerates skin aging. Ultraviolet rays can cause skin cells to wither prematurely or even lesions, accelerate the oxidation process, decompose collagen and other cell sec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/08A61Q19/02
CPCA61K8/19A61K8/347A61K8/44A61K8/73A61K8/97A61K2800/82A61Q19/02A61Q19/08
Inventor 朱剑虹王智富朱侗明
Owner SHANGHAI SHENYIN BIOTECH
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