Biomass carbon dot fluorescent probe for ratiometric quantitative detection of doxorubicin and its preparation method and application
A technology for quantitative detection of biomass carbon, applied in the field of fluorescent probes, can solve the problems of complex preparation process, poor anti-interference ability, high technical requirements, etc., and achieve the effect of simple preparation method, strong anti-interference ability and low preparation cost
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Embodiment 1
[0032] Example 1: Preparation and characterization of biomass carbon dots
[0033] Step 1: Weigh 0.5 g of corncob granular powder, add 10 mL of ultrapure water to it, and heat and carbonize in a high-pressure reactor at 180 °C for 12 h to obtain a light yellow biomass carbon dot solution.
[0034] Step 2: After the reactor is cooled to room temperature, the light yellow solution is centrifuged at 8000 rpm for 10 minutes to obtain a clear solution of biomass carbon dots, which is then freeze-dried to obtain solid biomass carbon dot powder.
[0035] Step 3: Accurately weigh 0.1 g of biomass carbon dot solid powder into a beaker, add 10 mL of ultrapure water to it, stir to dissolve it fully, and obtain a biomass carbon dot stock solution with a concentration of 10 mg / mL.
[0036] Characterization see figure 1 and figure 2 . figure 1 In the ultraviolet-visible absorption spectrum of biomass carbon dots, there are two obvious absorption peaks located at 281 nm and 323 nm, which...
Embodiment 2
[0037] Embodiment 2: Anti-interference experiment of doxorubicin detection
[0038] Step 1: Weigh drugs of different qualities (biotin, amoxicillin, lincomycin, penicillin G sodium, kanamycin, thiamine, ascorbic acid), add 10 mL of ultrapure water, and prepare a concentration of 0.01 mol / L drug stock solution.
[0039] Step 2, weighing amino acids of different masses (methionine, asparagine, glutamic acid, tyrosine, proline, isoleucine, phenylalanine, leucine, threonine, alanine, cysteine, serine, glycine, lysine, arginine, valine, histidine, glutamine, homocysteine), add 10 mL of ultrapure water to make up the concentration 0.01 mol / L amino acid stock solution.
[0040] Step 3, measure the fluorescence intensity of 0.148 mg / mL biomass carbon dot solution (2030 μL), denoted as F 0 ; Add 100 μL drug stock solution to it, the concentration of the drug at this time is 469 μmol / L, measure the fluorescence intensity of the solution at this time, denoted as F 1 ; add 10 µL doxor...
Embodiment 3
[0043] Embodiment 3: the linear equation of doxorubicin titration biomass carbon point
[0044] Step 1, measure the fluorescence intensity of 0.148 mg / mL biomass carbon dots, denoted as I 437 and I 553 .
[0045] Step 2, add doxorubicin stock solution dropwise to the above solution, and record the fluorescence intensity I 437 and I 553 , the fluorescence intensity changes see Figure 8 .
[0046] Step 3, using Origin software, fitting the change of fluorescence intensity (I 555 / I 435 ) and the linear equation between the concentration of doxorubicin, see Figure 9 and Figure 10 .
[0047] Figure 8 It shows that with the addition of doxorubicin stock solution, the fluorescence at 435 nm of the mixed solution of biomass carbon dots and doxorubicin is gradually quenched, and the fluorescence at 553 nm is gradually enhanced. Figure 9 , Figure 10 It is a linear relationship diagram between the change value of fluorescence intensity of biomass carbon dots and doxoru...
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