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A near-infrared fluorescent probe for detecting cyp 1A1 enzyme

A CYP1A1, fluorescent probe technology, applied in the field of near-infrared fluorescent probes, can solve the problems of organism interference and low selectivity, and achieve the effect of high selectivity

Active Publication Date: 2022-06-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Purpose of the invention: The present invention provides a near-infrared fluorescent probe for detecting CYP 1A1 enzymes, aiming at the problems of interference by organisms and low selectivity during detection caused by the green light of the CYP 1A1 enzyme fluorescent probe in the prior art, By modifying the structure of the fluorescent probe, the molecular structure can realize the specific detection of CYP 1A1 enzyme. At the same time, the fluorescence wavelength emitted by the fluorescent group in the fluorescent probe is in the near-infrared region, so it can avoid being affected by the green light of the organism. interference

Method used

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  • A near-infrared fluorescent probe for detecting cyp 1A1 enzyme
  • A near-infrared fluorescent probe for detecting cyp 1A1 enzyme
  • A near-infrared fluorescent probe for detecting cyp 1A1 enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The fluorescent probe of the present invention 2-((6-((4-(2-bromoethoxy)benzyl)oxy)-2,3-dihydro-1H-xanthin-4-yl)methylene) Malononitrile (BrDXMM) is prepared by the following method:

[0031] 1 mmol of HDXMM (HDXMM of the present invention was prepared by the synthetic method disclosed in the following document: Sensors & Actuators: B. Chemical 273 (2018) 167-175), 2 mmol of potassium carbonate and 1.2 mmol of 4-(2-bromoethoxy) ) benzyl bromide was placed in a 25mL two-necked flask, then 8mL of N,N-dimethylformamide was added to the two-necked flask, the reaction mass was heated to 90°C under nitrogen protection, and the reaction was stirred for 1 hour; after the reaction Cooled to room temperature, the reaction solution was added to cold water, stirred vigorously, and a large amount of dark red solids were precipitated, filtered, and the filter cake was separated by column chromatography (developing solvent: ethyl acetate: petroleum ether=5:1, v:v ) to obtain a dark r...

Embodiment 2

[0032] Example 2: Determination of the selectivity of fluorescent probe BrDXMM for each isoform of CYP450

[0033]Preparation of phosphate buffer solution (PBS): Weigh sodium dihydrogen phosphate dihydrate (0.7410g), dodecahydrate disodium hydrogen phosphate (7.2495g) and magnesium chloride hexahydrate (0.2030g) with an analytical balance and place them in a 100mL beaker , add double distilled water (200 mL in total) to the beaker in 4 times to fully dissolve, let stand, transfer to a 250 mL volumetric flask, add double distilled water to make the volume, fully shake evenly, and then use a pH meter to check the pH of the buffer . Prepared to obtain PBS buffer (0.1M, pH 7.4, c(MgCl) 2 ) = 4.0 mM), stored at 4°C.

[0034] Preparation of reaction substrate solution: Weigh BrDXMM and dissolve it in dimethyl sulfoxide to prepare a mother solution with a concentration of 5 mM. Take 40 μL of the stock solution and add it to 4.96 mL of PBS buffer to prepare a 40 μM reaction substra...

Embodiment 3

[0037] Example 3: Chemical inhibition experiment of fluorescent probe BrDXMM

[0038] 100 μL of the above-mentioned glucose-6-phosphate solution, 50 μL of β-nicotinamide adenosine phosphate solution, 5 μL of glucose-6-phosphate dehydrogenase solution, and 5 μL of CYP 1A1 ( Final concentration 50nM), resveratrol (final concentration 25μM, CYP 1A1 specific inhibitor), after mixing, add 10μL, 40μM BrDXMM (final concentration 10μM), and finally diluted with PBS buffer to the corresponding concentration. The above mixture was then mixed and incubated in a constant temperature water bath at 37°C for 60 minutes. At the end of the incubation, dimethyl sulfoxide (200 μL) was added to the reaction system to stop the reaction and mix well. The mixture was cryogenically centrifuged (13,300 × g, 4 °C) for 20 min, and the supernatant was taken for fluorescence analysis, via image 3 It can be seen that the fluorescent probe BrDXMM of the present invention indeed produces a fluorescent res...

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Abstract

The invention discloses a near-infrared fluorescent probe for detecting CYP 1A1 enzyme. The molecular structure of the fluorescent probe can be modified to enable the specific detection of CYP 1A1 enzyme, thereby having high selectivity; The fluorescence wavelength emitted by the fluorophore in the probe is in the near-infrared region, so it can avoid the interference of the green light emitted by the organism when performing fluorescence detection; secondly, the fluorescence performance of BrDXMM is very weak, while HDXMM has good fluorescence performance under the same conditions. Fluorescence emission spectrum characteristic (590-800nm), thereby can distinguish well, therefore fluorescent probe of the present invention all has high signal-to-noise ratio in vivo and in vitro; And fluorescent probe of the present invention also has good biocompatibility, Incubate the cells with a medium containing BrDXMM (100 μM) for 24 hours, and the cell activity is still greater than 85%; the fluorescent probe of the present invention can be used to recombine single enzymes in solution, live cells, isolated tissues, live zebrafish and tumor-bearing nude mice Determination of CYP 1A1 enzyme activity in vivo, with a wide range of applications.

Description

technical field [0001] The present invention relates to a near-infrared fluorescent probe for detecting CYP 1A1 enzyme. Background technique [0002] Cytochrome P450 enzymes (CYP450) are a superfamily of heme-containing monooxygenases and are the most important metabolic enzymes in living organisms. CYP450 enzymes are involved in the biotransformation of carcinogens, chemicals, drugs and many endogenous compounds, and play an important role in maintaining the normal physiological functions of organisms. The phase I reaction catalyzed by CYP450 enzymes is a key step in the metabolism of exogenous and endogenous compounds in the human body. It is usually the rate-limiting step for drug clearance from the body and is critical to the half-life and clearance rate of compounds. However, there are great individual differences in the distribution of CYP 1A1, and the expression of CYP 1A1 enzymes is closely related to factors such as heredity, gender, age, disease, environment, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/82C09K11/06G01N21/359G01N21/64
CPCC07D311/82C09K11/06G01N21/6428G01N21/6486G01N21/359C09K2211/1088G01N2021/6439
Inventor 祁争健代艳鹏薛珂赵鑫鑫
Owner SOUTHEAST UNIV
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