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Silver nanoparticle-collagen gelatin scaffold material and preparation method thereof

A technology of collagen and silver nanoparticles, applied in the field of biomedical materials, can solve the problems of limited repair effect of a single gel material, hinder the normal healing process of tooth extraction wounds, and cannot ensure new bone formation, etc., to achieve good load stability, Excellent mechanical elasticity and toughness, the effect of promoting osteogenesis

Active Publication Date: 2022-03-22
长春市儿童医院(吉林省儿童医疗中心长春市儿科医学研究所)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Clinical research on periodontal tissue regeneration focuses on the use of scaffold materials. The role of this scaffold material is to plant seed cells on the root surface and leave a certain space for periodontal tissue regeneration. However, the existing scaffold materials It does not guarantee that it will promote new bone formation, but the presence of the material will hinder the normal healing process of the extraction wound
[0004] In terms of promoting bone healing, the prior art mostly uses a single natural gel material as a scaffold material to promote osteogenesis, such as chitosan, fibrin, gelatin, hyaluronic acid, etc., which are non-toxic and have excellent biocompatibility. However, the repair effect of a single gel material is relatively limited, and there are also defects such as poor biomechanical strength and insufficient mechanical properties.

Method used

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  • Silver nanoparticle-collagen gelatin scaffold material and preparation method thereof

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Embodiment 1

[0037] This embodiment relates to a silver nanoparticle-collagen gelatin scaffold material, and its preparation method comprises the following steps:

[0038] S1, collagen modification

[0039] (1) Weigh 8 g of type I collagen and dissolve it in 50 mL of dimethylformamide solvent. After fully stirring, add 0.5 mL of anhydrous triethylamine under ice-water bath conditions, keep stirring and ice-water bath conditions, and then Add 80 mL of dichloromethane solution containing 0.32 mL of acryloyl chloride dropwise. After the dropwise addition, continue to stir for 1-2 hours, and then continue to stir for 24 hours at room temperature. After the reaction is completed, place the mixed system in distilled water for dialysis to purify The product was lyophilized after dialysis to obtain product A;

[0040] (2) Weigh 1g of hydroxyethyl isobromobutyrate and 24g of anhydrous ε-caprolactone and fully dissolve them in 100mL of toluene solvent, add 2mL of stannous octoate as a catalyst, clo...

Embodiment 2

[0045] This embodiment relates to a silver nanoparticle-collagen gelatin scaffold material, the preparation method of which is the same as that of Example 1, the difference is that:

[0046] In the mixing system described in step S2, lipopolysaccharide and bacterial cellulose are also added, and polyethylene glycol and sodium phytate are added after fully mixing, and constant temperature incubation is carried out after continuing to stir and disperse;

[0047] Wherein, the mass ratio of the modified collagen to the lipopolysaccharide, the bacterial cellulose, the polyethylene glycol, and the sodium phytate is 100:1:1.8:0.6:0.05.

Embodiment 3

[0049] This embodiment relates to a silver nanoparticle-collagen gelatin scaffold material, the preparation method of which is the same as that of Example 1, the difference is that:

[0050] In the mixed system described in step S2, silver nanoparticles are not added, but slow-release fillers are added, and the preparation method of the slow-release fillers includes the following steps:

[0051] (1) disperse in calcium chloride solution with 5-10wt.% sodium alginate aqueous solution, make calcium alginate gel microsphere; Disperse described calcium alginate gel microsphere in tetraethyl orthosilicate In the ethanol solution, stir the reaction overnight, filter out the microspheres, wash with absolute ethanol, and then immerse in the mixed solution of sodium chloride and hydrochloric acid. The concentration of sodium chloride is 0.1mol / L, and the concentration of hydrochloric acid is 0.02mol / L. Filter out to obtain precursor microspheres;

[0052] (2) Weigh the N-carboxyl amin...

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Abstract

The invention provides a preparation method of a silver nanoparticle-collagen gelatin scaffold material, and belongs to the field of biomedical materials, and the silver nanoparticle-collagen gelatin scaffold material is prepared by taking type I collagen with high biocompatibility and degradability as a main body material through grafting modification of poly (epsilon-caprolactone) on the basis of the type I collagen. The preparation method comprises the following steps: preparing a tough hydrogel with strength and elasticity characteristics by using collagen as a raw material, specifically, carrying out polymerization modification on collagen by using acryloylated collagen and polycaprolactone-2-bromoisobutyric acid-2-hydroxyethyl ester as comonomers, and adding silver nanoparticles to promote formation of new bones, so as to prepare the tough hydrogel with strength and elasticity characteristics. The gelatin scaffold material with biocompatibility and excellent mechanical elasticity and toughness is prepared, osteogenic differentiation of various osteoblasts is promoted, and osteogenesis after alveolar bone regeneration or fracture is promoted.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a silver nanoparticle-collagen gelatin scaffold material and a preparation method thereof. Background technique [0002] Chronic periodontitis can lead to gingival inflammation, periodontal tissue destruction, alveolar bone loss, and even tooth loss. It is one of the most important factors causing tooth loss in healthy people. According to oral epidemiological survey results, the total prevalence of gum and periodontal disease in 35-44 years old is 97.2%, while the total prevalence of gum and periodontal disease in 67-74 years old is as high as 99.4%. Periodontal disease is not only the primary cause of tooth loss in adults, but also an important factor affecting the functions of important organs such as the heart, lungs, and kidneys. According to foreign research reports, the probability of coronary heart disease and stroke in patients with periodontitis is 1.4 times and 2.1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/04A61L27/20A61L27/18A61L27/50A61L27/52A61L27/54
CPCA61L27/24A61L27/047A61L27/20A61L27/18A61L27/50A61L27/52A61L27/54A61L2400/12A61L2300/104A61L2430/12A61L2300/602A61L2300/412C08L67/04C08L71/02C08L1/02C08L5/00C08L5/04
Inventor 崔钊许云鹤
Owner 长春市儿童医院(吉林省儿童医疗中心长春市儿科医学研究所)