The invention discloses a stereotaxic cap for positioning in brain surgery and a manufacturing method thereof. The stereotaxic cap includes a cap body and at least one positioning fragment, wherein the cap body is provided with a support frame, and the cap body is divided into 8 fragments area; positioning fragments are set in each fragment area; the positioning fragments are at least one of left front fragments, right front fragments, left rear fragments, right rear fragments, left rear lower fragments, right rear lower fragments and two side fragments; Metal stickers for marking the inner apical point, posterior coronal point, and superior sagittal point are pasted on the positioning fragment; metal wires for marking AB, CD, EF, GH, KI, MK, MN, and coronal line are embedded in the positioning fragment. The present invention is an assembled structure, and positioning posts, anatomical body surface landmark positioning points, and positioning lines are arranged on the positioning sheet, and the marking is intuitive, easy to use, simple to operate, low in price, high in accuracy, and strong in practicability, and can partially replace the navigation function , easy to expand to the majority of grassroots hospitals.