The invention discloses an experimental beagle feeding management method. The method comprises the following steps that (1) female dogs during a pregnancy period and a suckling period are fed, specifically, sufficient uninterrupted drinking water and softened feed are provided for the female dogs during the pregnancy period and the suckling period, wherein the softened feed is prepared by adding milk powder and boiled water in standard experimental animal feed; and (2) puppies are fed, three days after the newborn puppies are born, the temperature in a delivery box is controlled to be kept within 26-32 DEG C, the softened feed is additionally added when the puppies are four weeks old, delactation is started when the puppies are 8 weeks old, after delactation, male dogs and female dogs areseparated fore feeding, individual marking is carried out, individual files are established, and a scientific immune procedure is started when the puppies are four weeks old. The feeding management method has the advantages of being clear and definite in flow, fine in control, easy to operate, scientific, reasonable, capable of improving the health level of beagles and capable of effectively decreasing various morbidity of the beagles, the experimental beagles obtained by feeding management are healthier, more stable and more uniform, and the method can better serve the bio-pharmaceutical experiments.