Serum-free culture medium for culturing DF-1 cells and preparation method of serum-free culture medium
A serum-free medium, DF-1 technology, applied in the field of serum-free medium for culturing DF-1 cells and its preparation, can solve the problems of low cell concentration, low virus titer, cumbersome culture process, etc. Ease of use, improved product quality, high cell density
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[0048]Example 1
[0049]This specific embodiment provides a serum-free medium for culturing DF-1 cells, which is composed of amino acids, vitamins, salts, lipids, trace elements, buffers, protein hydrolysates, acid-base indicators and other additives. Sub-group, where
[0050]Amino acids are alanine, arginine, asparagine, aspartic acid, cystine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine , Lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine;
[0051]Vitamins are biotin, folic acid, nicotinamide, pyridoxine, thiamine and lipoic acid;
[0052]Salts are magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium pyruvate;
[0053]Lipids are cholesterol, tocopherol acetate, myristic acid, palmitic acid, palmitoleic acid, stearic acid, Tween and block polyether F68;
[0054]Trace elements are copper sulfate, ferric nitrate, ferrou...
Example Embodiment
[0070]Example 2
[0071]This specific embodiment provides a serum-free medium for culturing DF-1 cells, and the specific content of each component in the serum-free medium for culturing DF-1 cells is:
[0072]Amino acid part:
[0073]Alanine 4 mg / L,
[0074]Arginine 100 mg / L,
[0075]Asparagine 6 mg / L,
[0076]Aspartic acid 9 mg / L,
[0077]Cystine 15 mg / L,
[0078]Cysteine 20 mg / L,
[0079]Glutamic acid 7 mg / L,
[0080]Glutamine 200 mg / L,
[0081]Glycine 10 mg / L,
[0082]Histidine 20 mg / L,
[0083]Isoleucine 30 mg / L,
[0084]Leucine 50 mg / L,
[0085]Lysine 100 mg / L,
[0086]Methionine 15 mg / L,
[0087]Phenylalanine 30 mg / L,
[0088]Proline 15 mg / L,
[0089]Serine 20 mg / L,
[0090]Threonine 30 mg / L,
[0091]Tryptophan 15 mg / L,
[0092]Tyrosine 40 mg / L,
[0093]Valine 40 mg / L.
[0094]The vitamin content is:
[0095]Biotin 0.003 mg / L,
[0096]Folic acid 2 mg / L,
[0097]Niacinamide 5 mg / L,
[0098]Pyridoxine 2 mg / L,
[0099]Thiamine 2 mg / L,
[0100]Lipoic acid 0.2 mg / L.
[0101]The content of the salt is:
[0102]Magnesium chloride 30 mg / L,
[0103]Magnesium sulfate 30 mg / L,
[0104]...
Example Embodiment
[0137]Example 3
[0138]This specific embodiment provides another serum-free medium for culturing DF-1 cells, and the specific content of each component in the serum-free medium for culturing DF-1 cells is:
[0139]Amino acid part:
[0140]Alanine 8 mg / L,
[0141]Arginine 200 mg / L,
[0142]Asparagine 20 mg / L,
[0143]Aspartic acid 16 mg / L,
[0144]Cystine 25 mg / L,
[0145]Cysteine 35 mg / L,
[0146]Glutamic acid 15 mg / L,
[0147]Glutamine 800 mg / L,
[0148]Glycine 25 mg / L,
[0149]Histidine 50 mg / L,
[0150]Isoleucine 60 mg / L,
[0151]Leucine 70 mg / L,
[0152]Lysine 150 mg / L,
[0153]Methionine 30 mg / L,
[0154]Phenylalanine 50 mg / L,
[0155]Proline 30 mg / L,
[0156]Serine 40 mg / L,
[0157]Threonine 60 mg / L,
[0158]Tryptophan 30 mg / L,
[0159]Tyrosine 70 mg / L,
[0160]Valine 70 mg / L.
[0161]The vitamin content is:
[0162]Biotin 0.01 mg / L,
[0163]Folic acid 5 mg / L,
[0164]Niacinamide 10 mg / L,
[0165]Pyridoxine 5 mg / L,
[0166]Thiamine 5 mg / L,
[0167]Lipoic acid 0.5 mg / L.
[0168]The content of the salt is:
[0169]Magnesium chloride 100 mg / L,
[0170]Magnesium sulfate 100 ...
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