The invention belongs to the technical field of anesthetic equipment, and particularly relates to an anesthetic bed buckle fixtor and assembling and using methods thereof. The anesthetic bed buckle fixtor comprises an inverted U-shaped fixed base. The fixed base comprises a fixed base body and fixing plates located on the front end face and the rear end face of the fixed base body respectively. Aconnecting clamping groove penetrating through the fixed base front and back is formed in the top of the fixed base, first grooves are formed in the left sides of the front end face and the rear end face of the fixed base body, and second grooves are formed in the portions, in the lower portions of the first grooves, of the fixed base body. A rubber ball is embedded into the inner side face of anarc-shaped clamping plate, a hemispherical groove is formed in the left portion of the end face of the end, away from the fixed base body, of the arc-shaped clamping plate, second threaded holes are formed in the portions, corresponding to the hemispherical groove, of the right portions of the front end faces of the fixing plates, bulb plungers matched with the hemispherical groove are arranged inthe second threaded holes, a triangular plate is arranged on the right end face of the arc-shaped clamping plate, and a round notch is formed in the right lower end of the triangular plate. The anesthetic bed buckle fixtor has the advantages that the problems that fixation is inconvenient, fixation is unstable, and the application range is narrow due to the structure defects of an existing bucklefixator are solved.