The invention relates to the technical field of
gene engineering, in particular to a forest tree exogenous
gene integration method based on
agrobacterium-mediated transient transformation. Connecting an exogenous
gene with a vector to obtain an exogenous
gene vector; transferring the exogenous
gene vector into
escherichia coli, extracting recombinant plasmids, and then transforming the recombinant plasmids into
agrobacterium tumefaciens to obtain exogenous gene overexpressed
agrobacterium tumefaciens; the exogenous gene overexpression agrobacterium infection
plant is subjected to co-culture, and a co-cultured
plant is obtained; treating the co-cultured
plant into an explant, carrying out primary differentiation culture to obtain an adventitious
bud, and carrying out strong
seedling culture on the adventitious
bud; and applying screening pressure to the strain with low overexpression multiple to perform secondary differentiation treatment so as to improve the expression quantity and the like. The invention not only has wide application prospect in genetic improvement of white birch, but also provides a new technical path for
molecular breeding, gene function research and
genome editing of other woody plants, and has remarkable scientific research value and economic application potential.