The invention particularly relates to a tissue multi-
chromophore in-vivo
quantitative imaging method based on a probability generation model. The tissue multi-
chromophore in-vivo
quantitative imaging method comprises the following steps: acquiring a multi-
wavelength photoacoustic initial
sound pressure reconstruction diagram of a target
biological tissue area; blind unmixing
processing is carried out on the multi-
wavelength photoacoustic initial
sound pressure reconstruction diagram, and spectral characteristics and concentration distribution of all chromophores in the target
biological tissue are initialized; converting the multi-
wavelength photoacoustic initial
sound pressure reconstruction diagram into a logarithmic multi-wavelength photoacoustic initial sound pressure reconstruction diagram through an LOG logarithmic decoupling method; constructing a probability generation model based on a variational auto-
encoder, inputting the logarithmic multi-wavelength photoacoustic initial sound pressure reconstruction diagram and the initialized spectral characteristics and concentration distribution into the probability generation model based on the variational auto-
encoder, solving a new
light absorption coefficient matrix, reconstructing a logarithmic multi-wavelength photoacoustic initial sound pressure reconstruction diagram by the
light absorption coefficient matrix, and obtaining a logarithmic multi-wavelength photoacoustic initial sound pressure reconstruction diagram. And thus, the
chromophore in-vivo spectrum and concentration image output by the model are constrained. And the precision and robustness of structure distinguishing in the complex
biological tissue are improved.