The present invention discloses an accumulated liquid sampling auxiliary device and a use method thereof. The accumulated liquid sampling auxiliary device comprises a needle cylinder, a first liquid collecting cavity is arranged in the needle cylinder, one end of the needle cylinder is provided with a connecting pipe, an elastic arc-shaped plate sleeves the connecting pipe positioned in the firstliquid collecting cavity, sliding blocks are arranged on both ends of the arc-shaped plate, limiting grooves are also arranged in an inner wall of an end part of the needle cylinder, when the arc-shaped plate moves towards the end part of the needle cylinder, the two sliding blocks can move towards both sides of the inner wall of the needle cylinder, side walls of the sliding blocks are respectively provided with a first movable rod, the first movable rods are connected with the sliding blocks in a sliding manner, both sides of the inner wall of the needle cylinder are respectively provided with a first cavity body, a first movable plate and a second movable rod are also arranged in each first cavity body, and each second movable rod penetrates into the first liquid collecting cavity; a sampling rod is also arranged in the needle cylinder, the sampling rod is internally provided with a second liquid collecting cavity, the second liquid collecting cavity is internally provided with a second piston and a second piston rod, both sides of an outer wall of the sampling rod are provided with limiting blocks, limiting blind holes are arranged on side walls of the limiting blocks, and an unblocking assembly is also arranged inside the end part of the needle cylinder.