The invention belongs to the field of biotechnology instruments, and particularly relates to a sample grinder for plant or animal tissue. The sample grinder is composed of a hanging mill (1), a linkage line (2), a funnel (3) and a funnel plug (4). The hanging mill (1) is connected with a motor through the linkage line (2). A base plate (13) below the hanging mill (1) is matched with the funnel (3) in a threaded manner. Vertical grain (32) is arranged on the outer side of the upper portion of the funnel (3). An inner spherical surface (33) of the funnel (3) is smooth. A funnel opening (34) is arranged at the outer bottom of the funnel (3), and cross grain is arranged on the outer side of the funnel opening (34). The upper surface of the funnel plug (4) is a spherical surface, and spring pieces (42) are arranged on the side face of the funnel plug (4). The funnel plug (4) is buckled and plugged into the funnel opening (34) at the bottom of the funnel (3), and the upper spherical surface (41) of the funnel plug (4) coincides with the inner spherical surface (33) of the funnel (3). According to the sample grinder, sample grinding is thorough, time is short, samples and liquid nitrogen are saved, the probability of cross contamination is lowered, and the risk that a sample pipe explodes is eliminated.