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Fermentation trace element premix and preparation method thereof as well as fermentation composite premix and preparation method thereof

A composite premix, trace element technology, applied in animal feed, animal feed, applications, etc., can solve problems such as toxicity, impact on human health, environmental pollution, etc.

Active Publication Date: 2015-07-29
王升平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, trace element premixes and compound premixes are not absorbed by animals due to the addition of a large amount of copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, magnesium sulfate, calcium carbonate, calcium hydrogen phosphate, zinc oxide, etc. Copper, iron, zinc, magnesium, etc. are discharged into the environment with feces, causing environmental pollution. At the same time, the raw materials of these substances contain a lot of heavy metal elements (such as cadmium, lead, chromium, etc.), and these heavy metal elements are discharged into the environment with feces ( water or soil), causing an increase in the heavy metal content of crops, therefore, it is necessary to reduce the amount of these raw materials added in the premix to improve the absorption efficiency of these elements by animals; the raw materials for adding trace elements are generally sulfates, such as copper sulfate , ferrous sulfate, zinc sulfate, etc., resulting in excessive sulfate content, causing animal hair growth, and has a toxic effect. The conversion of sulfate radicals into hydrogen sulfide and other content substances through fermentation can solve this problem very well; secondly , the extensive use of antibiotics has led to a rapid increase in drug-resistant bacteria, and antibiotics remain in meat and eggs, affecting human health

Method used

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  • Fermentation trace element premix and preparation method thereof as well as fermentation composite premix and preparation method thereof
  • Fermentation trace element premix and preparation method thereof as well as fermentation composite premix and preparation method thereof
  • Fermentation trace element premix and preparation method thereof as well as fermentation composite premix and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A fermented trace element premix is ​​made from the following raw materials in parts by weight:

[0078]

[0079] A preparation method for fermented trace element premix, the steps are:

[0080] 1. Prepare respectively the fermented liquid of Bacillus subtilis, Lactobacillus plantarum and saccharomyces;

[0081] 2. Add the above-mentioned raw materials to the blender or mixer according to the addition ratio, except for the fermentation liquid of Bacillus subtilis, the fermentation liquid of Lactobacillus plantarum, and the fermentation liquid of Candida utilis, and mix well for about 3-10 minutes;

[0082] 3. Add the prepared Bacillus subtilis fermentation broth, Lactobacillus plantarum fermentation broth, and Candida utilis fermentation broth, mix thoroughly for 3-10 minutes, and adjust the water content to 25-50%;

[0083] 4. After bagging, tie the mouth of the bag tightly, ferment at room temperature or in a greenhouse at 20-40 degrees for 2-7 days, dry or not. ...

Embodiment 11

[0088] A fermented composite premix, which is made of the following raw materials in parts by weight:

[0089]

[0090]

[0091] A preparation method for a fermented trace element premix and a fermented compound premix, the steps of which are:

[0092] 1. Strain selection: select Bacillus subtilis (hereinafter referred to as bacterial strain A), saccharomyces (hereinafter referred to as bacterial strain B), and lactic acid bacteria (hereinafter referred to as bacterial strain C) with good effect of degrading sulfate. Commercialized or publicly reported strains or strains with the functions screened in a conventional manner can accomplish the present invention.

[0093] 2. Liquid fermentation of bacterial strains: carry out conventional liquid fermentation on the selected bacterial strains A, B and C respectively to produce liquid fermented seed liquid.

[0094] The composition of the LB medium used for the strain A is: tryptone 10g, yeast extract 5g, sodium chloride 10g...

Embodiment 21

[0111] Preparation of strain A fermentation broth:

[0112] Get 2ml bacterial strain A (viable bacteria concentration is 10 10 CFU / ml), inoculated in 100ml medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 7.2, the rotation speed is 200-300r / min, and the fermentation time is 24-36h. The shake flask fermentation medium is LB medium, the ingredients are: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000ml, and the pH value is 7.2.

[0113] After the shake flask fermentation is completed, carry out the expansion culture of the fermenter. Take 100ml of the shake flask fermentation seed liquid and inoculate it into a 10L fermenter with a liquid volume of 5L. min, ferment for 24-36 hours. The composition of the medium in the pilot test of the fermenter was the same as that of the shake flask fermentation medium. After the fermentation, the medium was kept at 4°C for later use.

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Abstract

The invention discloses a fermentation trace element premix and a preparation thereof as well as a fermentation composite premix and a preparation method thereof. The fermentation trace element premix comprises raw materials of bacillus subtilis, lactobacillus plantarum, candida utilis, a fermentation carrier, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, sodium selenite, calcium carbonate, calcium hydrophosphate, cobaltous sulfate and potassium iodide. The preparation method of the fermentation trace element premix comprises the following steps of uniformly mixing the raw materials according to a certain proportion; adjusting the water content; packaging the mixture with an airtight packaging bag; tightening a bag mouth and fermenting; drying or not drying a fermented product to obtain the fermentation trace element premix. The preparation method of the fermentation composite premix comprises the following steps of mixing the prepared fermentation trace element premix with vitamin, edible salts, an enzymic preparation, a non-nutritional additive, amino acid and the like according to the certain proportion, and packaging. According to the fermentation trace element premix and the fermentation composite premix, the formula is reasonable, the use is convenient, the animal feed intake is improved, the utilization rate of feed is improved, the animal diarrhea rate is reduced, the immunity and disease resistance of animals are improved, the environmental pollution is reduced, and the sustainable development of animal husbandry is promoted.

Description

technical field [0001] The present invention relates to the technical field of feed additives and additive premixes, more specifically to a fermented trace element premix and a fermented compound premix, and also to the preparation of a fermented trace element premix and fermented compound premix Method and application, it is suitable for the production of trace element premix and compound premix. Background technique [0002] Premixes are the core components in the production of complete compound feeds or feed concentrates. The traditional trace element premix is ​​made by mixing the raw materials of copper, iron, zinc, manganese, magnesium, selenium, cobalt, iodine and other elements with the carrier. The production method of the traditional compound premix is ​​to prepare any two or more types of vitamin premix, trace element premix, various amino acids, salt, calcium, phosphorus, enzymes, antibiotics and other raw materials with the carrier in a certain proportion. mix...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/175
Inventor 王升平
Owner 王升平
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