The invention discloses a glass tube sealing device for a medical glass tube sealing assembly machine in the technical field of medical glass tube production and processing. The glass tube sealing device comprises a base, a first rotating plate, a second rotating plate, a first positioning assembly, a second positioning assembly and a driving assembly. According to the glass tube sealing device, the position of a glass tube is fixed through the first positioning assembly, the position of a sealing piece is adjusted and positioned through the action of the second positioning assembly and a second cam, a first roller moves along the wheel surface of a first cam, the first cam enables a rack to move, the first positioning assembly and the glass tube are driven to rotate, a tube opening rotates downwards, a pressing plate drives a sliding plate to move downwards, the tube opening of the glass tube is tightly pressed on the sealing piece, so that the sealing piece is tightly clamped on theglass tube, and sealing is completed; and one side, in contact with the glass tube opening, of the sealing piece is placed upwards and is not in contact with other structures, so that the sealing quality is prevented from being influenced by dust or other sundries and the like adhered to the sealing piece.