The invention belongs to the wireless communication Internet of vehicles technology field and particularly relates to a demodulation reference signal mode setting method for an LTE-V2X system. According to a frame structure set based on the method, the DMRS interval in the frequency domain direction is 6, and the DMRS interval in the time direction is 1; or the DMRS interval in the frequency domain direction is 6 or 3, the SC-FDM symbol position in the time direction is divided into two portions, including the front portion and the back portion, DMRS are distributed from the initial portions of the front portion and the back portion, and the DMRS interval of each portion is 2. The method is advantaged in that three types of LTE-V2X system demodulation reference signal design modes are proposed, density of demodulation reference signals in the time domain direction is enhanced, relatively high Doppler of a V2X system can be better responded, moreover, the intervals of the demodulation reference signals in the frequency domain direction are enhanced, confrontation to the relatively large time delay is further guaranteed, cost of the DMRS can be reduced to the maximum degree, and thefrequency spectrum utilization rate is improved.