The invention relates to the technical field of industrial conveyance, and discloses a rail trolley for industrial tool conveyance and a rail. Guide rail plate frames are connected through locking mechanisms, I-shaped guide rails are symmetrically fixed to the upper sides of the guide rail plate frames, a rail trolley mechanism is clamped on the upper sides of the I-shaped guide rails, a supporting seat is fixed to the upper side of the rail trolley mechanism, the supporting seat is combined with a rotating disc in a clamped manner through a limiting clamping groove, and a supporting and adjusting mechanism is fixed to the upper side of the rotating disc. Through matching and combination between packaging clamping blocks and packaging clamping grooves, under locking connection of the locking mechanisms, the work personnel can conveniently assemble the guide rails for use, and meanwhile, after use, the work personnel can conveniently disassemble and recycle the guide rails; and in the use process of the rail trolley, a rotating motor drives the rotating disc to rotate, under the adjusting and supporting of the supporting and adjusting mechanism, the work personnel can conveniently adjust the direction and the spacing distance of roller supports.